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A kind of method for improving the yield of Inonotus obliquus in liquid fermentation of Inonotus obliquus

A technology of Inonotus obliquus and Inonotus obliquus is applied in the field of increasing the yield of Inonotus obliquus in liquid fermentation of Inonotus obliquus, and can solve the problems of inability to meet the production requirements, high cost of methyl jasmonate and fatty acids, and achieve low cost. , the effect of simple post-processing and high resource utilization

Active Publication Date: 2021-01-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the SCI paper "Stimulated production of triterpenoids of Inonotus obliquus using methyl jasmonate and fatty acids" published by Xiangquan Xu et al., the fermentation medium and fermentation conditions were optimized based on the total triterpenoid production of Inonotus obliquus fermentation, and exogenous Factors methyl jasmonate and fatty acids, using Inonotus obliquus (CBS314.39) to ferment and produce Inonotus obliquus alcohol, the highest unit yield is only 2.5mg / g, the highest yield is only 23.26mg / L, and the added jasmonic acid The cost of methyl esters and fatty acids is high and cannot meet the needs of industrial production

Method used

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  • A kind of method for improving the yield of Inonotus obliquus in liquid fermentation of Inonotus obliquus
  • A kind of method for improving the yield of Inonotus obliquus in liquid fermentation of Inonotus obliquus
  • A kind of method for improving the yield of Inonotus obliquus in liquid fermentation of Inonotus obliquus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Fermentative production of Inonotiol by different nitrogen sources

[0044] Seed liquid medium: glucose 30g / L, soybean peptone 2g / L, yeast extract powder 2g / L, magnesium sulfate heptahydrate 0.5g / L, potassium dihydrogen phosphate 1g / L, calcium chloride 0.1g / L, pH natural .

[0045] Fermentation medium: Nitrogen source (yeast extract powder, soybean peptone, corn steep liquor powder, ammonium citrate, diammonium hydrogen phosphate, ammonium sulfate, sodium nitrate) 6g / L, glucose 30g / L, potassium dihydrogen phosphate 1.5g / L, magnesium sulfate 0.5g / L, calcium chloride 0.1g / L.

[0046] (1) Preparation of seed solution

[0047] After activation, Inonotus obliquus (CFCC 83414) was inoculated into the seed liquid medium, and the shake flask was fermented and cultured for 5 days under the condition of rotating speed of 160r / min and temperature of 27°C.

[0048] (2) Fermentation culture

[0049] The liquid seed liquid of Inonotus obliquus in the logarithmic growth...

Embodiment 2

[0052] Example 2: Fermentative production of inonotuja alcohol with different compound nitrogen source additions

[0053] Fermentation medium: compound nitrogen source (mass ratio of corn steep liquor powder and sodium nitrate is 1:1) (3, 6, 9, 12, 15 g / L respectively), glucose 30 g / L, potassium dihydrogen phosphate 1.5 g / L L, magnesium sulfate 0.5g / L, calcium chloride 0.1g / L.

[0054] The Inonotus obliquus (CFCC 83414) seed liquid that is in the logarithmic growth phase that obtains in embodiment 1 inserts the compound nitrogen source (corn steep liquor powder: sodium nitrate=1 1) In the fermentation medium, under the condition of a temperature of 27° C. and a rotation speed of 160 rpm, the fermentation culture was carried out for 10 days. The addition amount of nitrogen source was 3, 6, 9, 12, 15g / L respectively. After the fermentation, the content of inotolinol was measured. The optimal amount of compound nitrogen source added was 3g / L, and the yield of inotolinol was 14...

Embodiment 3

[0057] Example 3: Fermentative production of inotolin alcohol by different carbon sources

[0058] Fermentation medium: carbon source (glucose, rice flour, maltose, corn flour, potato starch, sucrose, dextrin) 30g / L, corn steep liquor powder 1.5g / L, sodium nitrate 1.5g / L, dihydrogen phosphate Potassium 1.5g / L, magnesium sulfate 0.5g / L, calcium chloride 0.1g / L.

[0059] The Inonotus obliquus (CFCC 83414) seed solution in the logarithmic growth phase obtained in Example 1 was inserted into a 30 g / L different carbon source type fermentation medium with a volume ratio of 15%, at a temperature of 27° C. Under the condition of rotating speed of 160rpm, the fermentation culture was carried out for 10 days. The carbon sources were glucose, rice flour, maltose, corn flour, potato starch, sucrose, and dextrin. After the fermentation, the content of inotolinol was measured. Glucose was the best carbon source, and the yield of inotolin was 10.98mg / L.

[0060] Table 3 Fermentation resu...

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Abstract

The invention discloses a method for increasing the alcohol production of Inonotus obliquus in liquid state fermentation, and belongs to the technical field of microbial fermentation. The invention aims at optimizing the culture medium and culture conditions for liquid fermentation of inotolin, a substance with anticancer activity. Based on this, exogenous factors were added as a means of regulating the production of inonotofaciol. After screening the exogenous factors, our laboratory finally selected geraniol as the best exogenous factor. Geraniol is a relatively cheap common industrial raw material and the added concentration of geraniol is very low. Finally, the highest unit yield of inotolin was 9.39mg / g, and the highest yield was 42.02mg / L. The invention has the advantages of low equipment investment, simple aftertreatment, basically no pollution, low cost, high resource utilization rate, and can obviously increase the yield of inonotus obliquus alcohol in liquid state fermentation by Inonotus obliquus.

Description

technical field [0001] The invention relates to a method for increasing the alcohol production of Inonotus obliquus in liquid state fermentation, and belongs to the technical field of microbial fermentation. Background technique [0002] Inonotus obliqus (Fr.) Pilat, also known as Chaga (Inonotus obliqus (Fr.) Pilat), is a precious medicinal and edible fungus, and its host is birch. It is used as an anticancer medicine in Russian folk. Inonotus obliquus contains a variety of pharmacologically active chemical components such as triterpenes, polysaccharides, and polyphenols, which have anti-cancer, anti-oxidation, hypoglycemic, and immune regulation functions. Inonotus obliquus alcohol is a lanostane-type triterpenoid compound unique to Inonotus obliquus, which has anti-cancer activities such as anti-blood cancer, human lung adenocarcinoma and cervical cancer. Therefore, the development of new drugs using inotolin as a raw material will show great development potential and e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/02C12R1/645
CPCC12P7/02
Inventor 张薄博管玉艳陈磊
Owner JIANGNAN UNIV