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Streptococcus thermophilus 210202 capable of high yielding of gamma-aminobutyric acids and application thereof

A technology of Streptococcus thermophilus and aminobutyric acid, applied in Streptococcus thermophilus 210202 and its application field, can solve problems such as low GABA content

Inactive Publication Date: 2019-09-10
GREENS BIOENG SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Relevant studies have confirmed that Streptococcus thermophilus has the ability to produce GABA, but the content of GABA is low

Method used

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  • Streptococcus thermophilus 210202 capable of high yielding of gamma-aminobutyric acids and application thereof
  • Streptococcus thermophilus 210202 capable of high yielding of gamma-aminobutyric acids and application thereof
  • Streptococcus thermophilus 210202 capable of high yielding of gamma-aminobutyric acids and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Screening and Identification of Streptococcus thermophilus

[0043] It was screened and obtained from the yogurt obtained from the yurts of the Kazak residents by the Sailimu Lake in Xinjiang, and 1g of the fresh milk sample was taken, and the obtained fresh milk was diluted with sterile normal saline at 1:10, and then extracted from -5, -6, -7 Take 100 μL samples from the three dilution gradient samples and spread them on the MRS plate medium, place them in an anaerobic environment, and culture them at 37°C for 48 hours. Afterwards, single colonies were picked on a plate with a -5 dilution gradient to be streaked and purified, and then cultured anaerobically at 37°C for 48 hours, and repeated three times to obtain strain 210202.

[0044] Using API50 reagent strips and 16S rRNA and other technologies to carry out taxonomic identification of the strain, it was identified as Streptococcus thermophilus species (such as figure 1 shown). The strain was then prese...

Embodiment 2

[0052] The screening method of the streptococcus thermophilus strain of embodiment 2 high yield GABA

[0053] The GABA production of Streptococcus thermophilus 210202 obtained in Example 1 was compared with other 74 strains of Streptococcus thermophilus in its own strain bank under the same culture conditions. Specific steps are as follows:

[0054] 1. Seed production

[0055] The 75 frozen Streptococcus thermophilus strains were activated by streaking on the MRS plate, and then single colonies were picked and cultured in M17 broth medium at 37°C for 24 hours. After two repetitions, the seed solution was prepared.

[0056] 2. Primary screening

[0057] The seed solution was added in an amount of 3% (v / v), and the activated seed solution was drawn and inoculated in the M17 synthetic medium, and after being placed at 37° C. for static cultivation for 72 hours, the GABA content in its fermentation liquid was determined. Completed the primary screening of high-yield GABA bacter...

Embodiment 3

[0060] Example 3 GABA content in skim milk detected by high performance liquid chromatography

[0061] In this example, high-performance liquid chromatography is used to detect commercially available GABA pure products, establish a standard curve of peak area and content, and determine the retention time; then detect the skim milk sample fermented by Streptococcus thermophilus for 48 hours, and calculate the peak area at a specific time , calculate the GABA content in the sample, and compare the GABA production of different Streptococcus thermophilus.

[0062] 1. Preparation of detection reagents

[0063] 1. 5% (v / v) acetic acid solution, 2mol / L sodium hydroxide solution, 5% (w / v) trichloroacetic acid solution.

[0064] 2. Borate buffer

[0065] Weigh 2.47g of boric acid, dissolve it in 80mL of distilled water, and then adjust the pH to 10.2 with 2mol / L sodium hydroxide solution.

[0066] 3. Preparation of derivatives

[0067] Weigh 0.1g of o-phthalaldehyde (OPA) into a 10...

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Abstract

The invention discloses streptococcus thermophilus 210202 capable of high yielding of gamma-aminobutyric acids and application thereof. The streptococcus thermophilus 210202 is preserved in the ChinaGeneral Microbiological Culture Collection Center (CGMCC) on October 15, 2018, and the preservation number is CGMCC No.16585. The streptococcus thermophilus strain 210202 capable of metabolism in cowmilk and high yielding of GABAs can be screened out from natural fermented food, and the streptococcus thermophilus strain 210202 is identified as a streptococcus thermophilus strain through the identification in morphology, metabolomics and genetics. The bacterial strain is capable of high yielding of the GABAs in a fermenting system containing sodium glutamate and dried skim milk reconstituted milk, and the content of the GABAs is 0.3273 g / L. The streptococcus thermophilus 210202 provides a new way for preparing a yoghurt starter or milk products rich in the gamma-aminobutyric acids, and hasgreat significance for industrial production of yogurt products with high-content GABAs.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a strain of Streptococcus thermophilus 210202 with high yield of γ-aminobutyric acid and its application. Background technique [0002] γ-aminobutyric acid (γ-aminobutyric acid, GABA) is the most important inhibitory neurotransmitter in the mammalian central nervous system, and GABA has an inhibitory effect on almost all neurons in the brain. The lack of GABA in the human body is the main cause of physical and mental discomfort. The importance of supplementing GABA is that it can relieve and inhibit excessive excitement and intense nerve information transmission, so as to calm people down and effectively relieve mental stress. [0003] Lactic acid bacteria, as a food safety grade (GRAS) microorganism, are widely used in the production of yogurt, cheese, pickles and other foods. Many studies have shown that lactic acid bacteria have glutamic acid decarboxylase acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/00A23C9/123C12R1/46
CPCC12P13/005A23C9/1238C12R2001/46C12N1/205A23V2400/249
Inventor 骆鹏飞俞兰秀
Owner GREENS BIOENG SHENZHEN
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