Application of pleurotus eryngii mycelium polysaccharide from solid-state fermentation

A technology of solid fermentation and mycelium, applied in medical preparations containing active ingredients, medical raw materials derived from fungi, metabolic diseases, etc., can solve the problem of less research on the mechanism of lipid-lowering activity, lack of active polysaccharides of edible fungi to lower lipids Mechanism and other issues, to achieve the effect of restoring the function of intestinal flora, reducing blood lipids and lipid accumulation in the liver

Pending Publication Date: 2019-09-13
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] A variety of edible fungus polysaccharides have been found to have lipid-lowering activity in vivo and in vitro. Among them, there are many reports on the isolation and identification of their active ingredients, but there are few studies on the mechanism of their lipid-lowering activity, especially the lack of active polysaccharides from edible fungi in animals. Lipid-lowering mechanism research

Method used

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  • Application of pleurotus eryngii mycelium polysaccharide from solid-state fermentation
  • Application of pleurotus eryngii mycelium polysaccharide from solid-state fermentation
  • Application of pleurotus eryngii mycelium polysaccharide from solid-state fermentation

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Experimental program
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Effect test

Embodiment 1

[0045] Embodiment 1: A kind of preparation method of Pleurotus eryngii mycelium solid fermented polysaccharide (PESF)

[0046] The method for producing the lipid-lowering active polysaccharide (PESF) by the solid fermentation method of Pleurotus eryngii mycelia is as follows:

[0047] 1.1 Mycelium solid fermentation production:

[0048] Transfer Pleurotus eryngii slant strains to PDA medium plates and culture them in dark at 25°C for 7 days for activation, then use a 1cm diameter puncher to punch holes on the plate covered with mycelium, and take 10 pieces of mycelium blocks to inoculate Put it into a sterilized and cooled glass bottle filled with solid fermentation medium, then tighten the vent cap, and carry out mycelia growth culture in a constant temperature and humidity incubator, dark culture, humidity 60%, temperature 25 ° C, culture 14 to 20 days , until the mycelium is covered with the medium, the solid fermentation of the Pleurotus eryngii mycelium is completed, and...

Embodiment 2

[0056] Example 2: Daily oral administration of PESF while providing mice with a high-fat diet can significantly reduce blood lipids

[0057] 2.1 Breeding of test mice:

[0058] The experimental mice were bred for one week to adapt to the conditions of the laboratory, and were reared in separate cages in the mouse breeding cage system, with 7 mice per cage. Keep the room clean, change the litter every two days, control the laboratory light for 12 hours and 12 hours for dark, the indoor temperature is 24±1℃, and the humidity is 55±10%.

[0059] 42 male ICR mice were weighed after adaptive feeding for one week, and randomly divided into 6 groups, with 7 mice in each group placed in a cage. Each group was numbered: normal group (N), high-fat group (F), High fat + PESF low concentration group (FT1), high fat + PESF high concentration group (FT2), high fat + simvastatin (Simvastatin) group (FS), PESF group (T). Except for the normal group and the PESF group fed with normal feed, t...

Embodiment 3

[0065] Example 3: PESF can regulate the content and function of some intestinal dysbiosis bacteria caused by high fat and return to normal

[0066] 3.1 Both high-fat diet and PESF significantly affected the composition of intestinal flora after intervention of high-fat diet:

[0067] The results of community composition analysis at the phylum level are shown in Table 2. The dominant flora in the community composition of each test group are mainly four phyla, which are Firmicutes, Bacteroidetes, and Bacteroides in order of proportion. ), Actinobacteria, Proteobacteria. In addition, there are a small number of other phyla (others in Table 2), including Verrucomicrobia, Tenericutes, Saccharibacteria, and Deferribacteres.

[0068] Table 2 Composition ratios of intestinal flora at the phylum level of mice in each test group on day 31 of PESF intervention with high-fat diet

[0069]

[0070] The results of the analysis of the community composition ratio at the genus level are s...

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Abstract

The present invention discloses an application of a pleurotus eryngii mycelium polysaccharide from solid-state fermentation (PESF). The active polysaccharide (PESF) with a hypolipidemic function is produced by a solid-state fermentation method of pleurotus eryngii mycelia. In a mouse model in vivo hypolipidemic mechanism, an oral administration of a certain dose of the PESF can regulate intestinalmicroflora, significantly promotes growth of Lactobacillus and inhibits major micro-organism genus of staphylococcus, corynebacterium, aerococcus, etc., thus restores intestinal flora imbalance caused by a high-fat diet to a certain degree, finally inhibits absorption of exogenous lipids in intestinal tract, and thus reduces accumulation of blood lipids and liver lipids in body. The pleurotus eryngii polysaccharide is analyzed and confirmed to have biofunctional activity of regulating structure and functions of the intestinal microflora, and a significant correlation is found between polysaccharide hypolipidemic activity and polysaccharide intestinal microflora-regulating function.

Description

technical field [0001] The invention belongs to the technical field of edible fungus deep processing, and in particular relates to the application of a solid fermented polysaccharide of Pleurotus eryngii mycelium. Background technique [0002] Hyperlipidemia is a disease characterized by elevated plasma total cholesterol (TC), total triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) and decreased high-density lipoprotein cholesterol (HDL-C). It is an important factor leading to atherosclerosis, which increases the risk of cardiovascular disease, central nervous system and other diseases, and has various complications. Current clinical lipid-lowering drugs can be divided into five categories according to their structural properties: statins, fibrates, niacin, resins, and cholesterol absorption inhibitors. Among them, statins were discovered in the 1970s. It is a major discovery in the treatment of dyslipidemia. It is an inhibitor of the rate-limiting enzyme of e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61K36/07A61P3/06
CPCA61K31/715A61K36/07A61P3/06
Inventor 魏华陆玲
Owner NANJING NORMAL UNIVERSITY
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