Application of galactose oligosaccharide and derivatives thereof as drugs for preventing and treating non-alcoholic fatty acid disease (NAFLD)
A galactooligosaccharide and non-alcoholic technology, which is applied in the direction of drug combinations, medical preparations containing active ingredients, antibacterial drugs, etc., can solve problems such as potential safety hazards, and achieve high safety, significant liver fat accumulation, and products good stability effect
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Embodiment 1
[0038] Example 1: Containing 6-O-sulfate-β-1,3-D-galactose (Gal6S) and α-1,4-L-3,6-inner ether galactose (AnG) thiogaran oligosaccharide ( Preparation of SAOs), oligosaccharide alcohols (SAOs-OH) and oligosaccharide acids (SAOs-OOH).
[0039] Prepare 10mg / mL aqueous solution with dilute sulfuric acid with a molar concentration of 0.1M to 1g of sulfur agar polysaccharide, heat it to 60°C, stir and degrade it for 1.5 hours, neutralize it with a NaOH aqueous solution with a molar concentration of 2M after cooling, collect the supernatant by centrifugation, and then Add 3 times the volume of 95% medical ethanol (volume concentration) at 4°C overnight, centrifuge to collect the precipitate, dissolve it with a small amount of water, and then use a 200Da dialysis bag to desalt it. Take 100 mg of SAOs and dissolve it in 10 mL of 100 mM NaBH 4 The aqueous solution (containing NaOH with a molar concentration of 100 mM) was reacted overnight at 4°C, and the pH was adjusted to 7.0 by add...
Embodiment 2
[0044] Example 2: Laver gum oligosaccharides (PYOs), oligosaccharide alcohols (PYOs) containing β-1,3-D-galactose (Gal) and 6-O-sulfate-α-1,4-galactose (Gal6S) -OH) and oligosaccharide acids (PYOs-OOH).
[0045] Prepare laver gum with dilute sulfuric acid with a molar concentration of 0.1M to form a 10mg / mL aqueous solution, heat it to 80°C, stir and degrade it for 2 hours, neutralize it with an aqueous NaOH solution with a molar concentration of 2M after cooling, collect the supernatant by centrifugation, and add 4 times the volume 95% medical ethanol was overnight at 4°C, the precipitate was collected by centrifugation and dissolved with a small amount of water, desalted with a 200Da dialysis bag, concentrated by rotary evaporation, and freeze-dried to obtain PYOs (such as figure 1 shown in A-D). Take 150 mg of PYOs oligosaccharides and dissolve it in 15 mL of NaBH with a molar concentration of 150 mM 4 The aqueous solution (containing NaOH with a molar concentration of 15...
Embodiment 3
[0050] Example 3: Agar oligosaccharides containing β-1,3-D-galactose (Gal) and α-1,4-L-3,6-galactose (AnG) and their oligosaccharide alcohols and oligosaccharides acid preparation.
[0051] Dissolve the agarose in hot water, use dilute hydrochloric acid with a molar concentration of 0.1M to prepare a 10mg / mL solution, stir and degrade it at 80°C for 0.5 hours, neutralize it with an aqueous NaOH solution with a molar concentration of 2M after cooling, collect the supernatant by centrifugation, and then Add 3.5 times the volume of 95% medical ethanol at 4°C overnight, collect the precipitate by centrifugation, dissolve it in water, use a 200Da dialysis bag to dialyze and desalt, concentrate by rotary evaporation and freeze-dry to obtain agar oligosaccharide AOs, and further reduce it with sodium borohydride to obtain agar oligosaccharide Alcohol AOs-OH, or agarooligosaccharide acid AOs-OOH obtained by oxidation with Benedict's reagent. The chemical structural formulas of agar o...
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