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A kind of double network silk fibroin hydrogel and preparation method thereof

A technology of silk fibroin and double network, which is applied in the field of double network silk fibroin hydrogel and its preparation, can solve problems such as poor tensile properties, achieve toughness and tensile properties, improve elastic modulus, and not Easily damaged broken effect

Active Publication Date: 2021-11-19
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The object of the present invention is to provide a kind of double network silk fibroin hydrogel and preparation method thereof, to overcome the defect of poor stretchability existing in existing silk fibroin hydrogel

Method used

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  • A kind of double network silk fibroin hydrogel and preparation method thereof
  • A kind of double network silk fibroin hydrogel and preparation method thereof

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preparation example Construction

[0029] The preparation method of double network silk fibroin hydrogel of the present invention comprises:

[0030] (1) Prepare a carboxylated silk fibroin solution; degumming, dissolving, and dialysis the silk to obtain a silk fibroin solution; chemically modifying the silk fibroin solution with borax, diazonium salt, and chloroacetic acid to obtain a concentration of 0.1%-50% carboxylated silk fibroin solution; dialyzing the carboxylated silk fibroin solution and freeze-drying to obtain carboxylated silk fibroin powder. The concentration of the carboxylated silk fibroin solution is 0.1%-50%. Within this concentration range, the silk fibroin can graft the host cyclodextrin and the guest adamantane to the greatest extent.

[0031] (2) Prepare silk fibroin-main solution; dissolve the aminated main body in dimethyl sulfoxide solution to prepare an aminated main solution with a concentration of 0.1-1000 mg / mL; dissolve EDC·HCl in two In methyl sulfoxide solution, make EDC·HCl sol...

Embodiment 1

[0037] (1) preparing carboxylated silk fibroin solution;

[0038] the silk through Na 2 CO 3 The solution was degummed, LiBr solution was dissolved, and deionized water was dialyzed to obtain a silk fibroin solution with a concentration of 1%. Mix and stir 5 mL silk fibroin solution with 2 mL, 10 M NaOH solution for 5 min, and then mix 4 mL, 1 M chloroacetic acid was added to the reaction mixture, mixed at room temperature for 50 min, and then 500 μL of 7 mg / mL NaH 2 PO 4 solution, reacted in an ice bath for 30 min to obtain a carboxylated silk fibroin solution; after dialysis and freeze-drying, a carboxylated silk fibroin powder was obtained.

[0039] (2) Prepare silk fibroin-cyclodextrin solution;

[0040] Dissolve aminated cyclodextrin in dimethyl sulfoxide solution to prepare an aminated cyclodextrin solution with a concentration of 1 mg / mL; dissolve EDC·HCl in dimethyl sulfoxide solution to prepare EDC•HCl solution with a concentration of 1 mg / mL; dissolve 1 mg of ca...

Embodiment 2

[0047] (1) preparing carboxylated silk fibroin solution;

[0048] the silk through Na 2 CO 3 The solution was degummed, LiBr solution was dissolved, and deionized water was dialyzed to obtain a silk fibroin solution with a concentration of 4%. Mix and stir 5 mL silk fibroin solution with 2 mL, 10 M NaOH solution for 5 min, and then mix 4 mL, 1 M chloroacetic acid was added to the reaction mixture, mixed at room temperature for 50 min, and then 500 μL of 7 mg / mL NaH 2 PO 4 solution, reacted in an ice bath for 30 min to obtain a carboxylated silk fibroin solution; after dialysis and freeze-drying, a carboxylated silk fibroin powder was obtained.

[0049] (2) Prepare silk fibroin-cyclodextrin solution;

[0050] Dissolve the aminated cyclodextrin in the dimethyl sulfoxide solution to prepare an aminated cyclodextrin solution with a concentration of 10 mg / mL; dissolve EDC·HCl in the dimethyl sulfoxide solution respectively to prepare into an EDC•HCl solution with a concentrati...

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Abstract

The invention discloses a double-network silk fibroin hydrogel and a preparation method thereof. The double-network silk fibroin hydrogel uses silk fibroin molecules as a matrix, and introduces a subject, a guest, and horseradish peroxidation into its amino acid side chain. A double-network silk fibroin hydrogel constructed with biomolecules. The invention uses the silk fibroin polymer with good biocompatibility as the matrix, introduces the host and the guest through chemical modification, and then introduces horseradish peroxidase and hydrogen peroxide into the system to prepare a double network silk fibroin hydrogel glue. The reversible binding between host and guest units and the crosslinking catalyzed by horseradish peroxidase endow the stability of the structure and properties of silk fibroin hydrogels. At the same time, the existence of the double network structure increases the cross-linking sites of the gel network, which greatly increases the elastic modulus of the silk fibroin hydrogel, thereby improving its tensile properties.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a double network silk fibroin hydrogel and a preparation method thereof. Background technique [0002] As a kind of soft material, hydrogel is formed by the cross-linking of hydrophilic polymer chains in water, which has a high water content and can transport oxygen and nutrients, similar to natural extracellular matrix, It has broad application prospects in tissue engineering, drug delivery, regenerative medicine and other fields. Silk fibroin is a natural high-molecular fiber protein extracted from silk, which has been certified by FDA and can be used in clinical medicine. Silk fibroin-based hydrogel has excellent biocompatibility and biodegradability. It can not only provide a suitable microenvironment for cell proliferation and differentiation, but also realize tissue repair and regeneration. It has important applications in the field of biomedicine value. Silk fibroi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L89/00C08L5/16C08K5/01C08J3/075
CPCC08J3/075C08J2389/00C08J2405/16C08K5/01
Inventor 黄晓卫
Owner QINGDAO UNIV
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