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Multifunctional lactobacillus fermentum CCFM1051 for relieving PFOA toxicity and fermented foods and application thereof

A technology of CCFM1051 and Lactobacillus fermentum, which is applied in the field of microorganisms, can solve the problems of expensive and difficult to obtain, and achieve the effect of inhibiting oxidative stress, wide application prospects, and alleviating the toxicity of PFOA

Active Publication Date: 2019-09-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these natural substances are expensive and difficult to obtain. In addition, due to the tolerance of the human body, it is not yet known whether a large amount of intake will cause potential harm to the human body.

Method used

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  • Multifunctional lactobacillus fermentum CCFM1051 for relieving PFOA toxicity and fermented foods and application thereof
  • Multifunctional lactobacillus fermentum CCFM1051 for relieving PFOA toxicity and fermented foods and application thereof
  • Multifunctional lactobacillus fermentum CCFM1051 for relieving PFOA toxicity and fermented foods and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1: Good PFOA adsorption capacity in vitro;

[0073] Cell adsorption Lactobacillus fermentum CCFM1051 was purified and activated, inoculated in MRS liquid medium at 1% (v / v) inoculum, and cultured at 37°C for 18 hours. Then centrifuge at 8000r / min for 5min to collect the bacteria, take the precipitate and clean it with normal saline, then continue to centrifuge at 8000r / min for 5min to remove the precipitate to obtain live bacteria cells, that is, wet bacteria. The wet bacteria were resuspended in 10mg / LPFOA solution, and the final bacterial concentration reached 1g dry bacteria / L (the wet bacteria were resuspended in PFOA-free ultrapure water as a blank control). Use 0.1M NaOH or HCl solution to quickly adjust the pH of the PFOA solution containing bacteria to 3.0, add a small amount of NaOH or HCl (less than 0.5ml), and its ionic strength has negligible influence on PFOA adsorption. Subsequently, the 250ml Erlenmeyer flask containing 100ml of the sample sol...

Embodiment 2

[0078] Embodiment 2: It has good ability to scavenge diphenyltrinitrophenylhydrazine free radical (DPPH) in vitro, scavenge hydroxyl free radical ability and reducing ability;

[0079] After fully mixing 1 mL of lactic acid bacteria complete cell suspension and 1 mL of freshly prepared DPPH absolute ethanol solution (0.2 mmol / L), react in the dark at 37°C for 30 min. Mix DPPH and PBS (pH7.2) as a control sample, and culture under the same conditions. After centrifuging at 7000×g for 10 min, measure the absorbance at 517 nm, and calculate the ability of lactic acid bacteria to scavenge DPPH free radicals according to the following formula:

[0080] DPPH radical scavenging rate (%)=[1-A517 (sample) / A517 (control)]×100%.

[0081] Mix 1 mL of 1,10-phenanthroline, 1 mL of LPBS (pH 7.2), 1 mL of lactic acid bacteria complete cell suspension or 1 mL of FeSO4 (referred to as "mixture 1"). Add 1mL of H2O2 to "Mixture 1", bathe in water at 37°C for 1.5h, measure the absorbance at 536n...

Embodiment 3

[0084] Example 3: Lactobacillus fermentum CCFM1051 can significantly improve spleen atrophy in mice exposed to PFOA;

[0085] Fifty 6-week-old male C57BL / 6J mice were randomly divided into five groups according to body weight after acclimatization for one week: control group, model group, quercetin intervention group, Lactobacillus fermentum CCFM1051 intervention group, LGG intervention group, each group Including 10 mice, animal grouping and treatment methods are shown in Table 2

[0086] Table 2 Grouping and processing methods of animal experiments

[0087]

[0088]

[0089] The mice in Example 3 were weighed on the 13th day, then euthanized, the spleen was taken out and weighed wet to calculate the organ coefficient, and the organ coefficient of the mouse spleen was calculated according to the following formula:

[0090] Spleen organ coefficient = wet weight of spleen / weight of mouse before euthanasia

[0091] Experimental results such as image 3 As shown, the res...

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Abstract

The invention discloses a multifunctional lactobacillus fermentum CCFM1051 for relieving PFOA toxicity and fermented foods and application thereof. The invention screens out probiotics which have highadsorption capacity on PFOA, do not colonize in human bodies and have high oxidation resistance; and the probiotics not only can inhibit oxidative stress caused by the PFOA, but also can fundamentally eliminate the PFOA in human bodies. The lactobacillus fermentum CCFM1051 can be used for preparing foods, health products and medicines with PFOA toxicity relieving effect, and has wide applicationprospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a multifunctional fermenting lactobacillus CCFM1051 for alleviating the toxic effect of PFOA, fermented food and applications thereof. Background technique [0002] Perfluorinated compounds have been widely used in various industries due to their hydrophobic and oleophobic properties, as well as good thermal stability, chemical stability and biological stability. Used in ready-to-wear (such as waterproof, stain-resistant outdoor clothing) and home textiles (such as carpets, furniture fabrics, etc.), takeaway food containers, personal care products (such as dental floss), and fire fighting foam. Among them, PFOA (PFOA), as one of the final conversion products of various fluorine compounds, can be enriched along the food chain. PFOA has been detected in various environmental media around the world, such as water, soil, atmosphere, dust, etc., as well as in animals and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L19/20A23C13/16A23C19/00A23C9/123A61K35/747A61P39/06A61P1/10A61P1/16A61P9/12A61P3/04C12R1/225
CPCA23L19/20A23C13/165A23C19/00A23C9/1234A61K35/747A61P39/06A61P1/10A61P1/16A61P9/12A61P3/04A23V2002/00C12R2001/225C12N1/205A23V2400/143A23V2200/30A23V2200/326A23V2200/332
Inventor 王刚陈卫梁席赵建新张灏
Owner JIANGNAN UNIV
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