Method for preparing medicine albumin nanoparticles

An albumin nanoparticle and a technology for preparing medicine, which are applied in the field of preparing medicine albumin nanoparticle, can solve the problems of low solubility, difficult vanillin, long time consumption and the like, and achieve the effects of fewer process steps, simple operation and environmental friendliness.

Inactive Publication Date: 2019-09-17
QIQIHAR MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this preparation method, the dialysis time is generally 24 hours, which takes a long time, and the protein particles may be degraded
In addition, vanillin is insoluble in cold water and has low solubility in hot water, which will affect its cross-linking efficiency

Method used

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  • Method for preparing medicine albumin nanoparticles
  • Method for preparing medicine albumin nanoparticles
  • Method for preparing medicine albumin nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Paclitaxel 0.4g

[0050] Bovine Serum Albumin 8g

[0051] Calcium chloride 0.08g

[0052] Preparation Process:

[0053] (1) Add paclitaxel 0.4g into 10ml of absolute ethanol to dissolve.

[0054] (2) Dissolve 8g of bovine serum albumin and 0.08g of calcium chloride in 400ml of water for injection.

[0055] (3) At room temperature, add the solution in step 2 into the plunger pump high-pressure homogenizer cup, and homogenize at 15MPa. At the same time, the ethanol solution of the drug in step 1 was quickly dripped into the homogenizer material cup by using a peristaltic pump, and the dripping speed was 10ml / min. After all the drug solution is dropped in, increase the homogenization pressure to 50MPa, and at the same time, increase the homogenization temperature to 65°C (heated by hot water circulation). After 3 cycles of homogenization at 50MPa, increase the pressure to 80MPa and perform 5 cycles of homogenization. The suspension was cooled to room temperature, pla...

Embodiment 2

[0058] Paclitaxel 0.15g

[0059] Human Serum Albumin 1.5g

[0060] Calcium acetate 0.15g

[0061] Tannic acid 0.3g

[0062] Preparation Process:

[0063] (1) Add 0.15g of paclitaxel into 3ml of absolute ethanol to dissolve.

[0064] (2) Dissolve 1.5g of human serum albumin and 0.15g of calcium acetate in 30ml of water for injection.

[0065] (3) At room temperature, put the solution in step 2 into the cup of a high-pressure micro-fluidic homogenizer, and homogenize at 15MPa. At the same time, the drug ethanol solution in step 1 was quickly dripped into the material cup by using a peristaltic pump, and the dripping speed was 10ml / min. After all the drug solution is dripped in, increase the homogenization pressure to 50MPa, and use the temperature control system to increase the temperature of the high-pressure homogenization to 70°C. After 3 cycles of 50MPa homogenization, increase the pressure to 100MPa, and homogenize for 3 times. cycle. Naturally cool the suspension to...

Embodiment 3

[0068] Doxorubicin 0.5g

[0069] Bovine Serum Albumin 10g

[0070] Calcium Lactate 0.5g

[0071] Preparation Process:

[0072] (1) Add 0.5 g of doxorubicin to 20 ml of dimethyl sulfoxide to dissolve.

[0073] (2) Add 10g of bovine serum albumin and 0.5g of calcium lactate into 200ml of water for injection to dissolve.

[0074] (3) At room temperature, add the solution in step 2 into the plunger pump high-pressure homogenizer cup, and homogenize at 15MPa. At the same time, the doxorubicin dimethyl sulfoxide solution in step 1 was quickly dropped into the homogenizer material cup by using a peristaltic pump, and the dropping speed was 10ml / min. After all the drug solution is dropped in, increase the homogenization pressure to 120MPa, and at the same time, increase the homogenization temperature to 70°C (heated by hot water circulation). Homogenize for 3 cycles. The suspension was cooled to room temperature, placed in a high-speed centrifuge, and centrifuged at 10,000 r / min...

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Abstract

The invention discloses a method for preparing medicine albumin nanoparticles, and belongs to the technical field of medicine preparations. According to the method disclosed by the invention, metal salt without biologic toxicity or a non-salt compound is used as a coagulator, a natural compound without biologic toxicity is used as a cross-linking agent, and a few of solvents which are nearly nontoxic, such as ethanol, are used as a medicine solvent. Nanoparticles are formed through energy instantaneously released through cavitation. The preparation method of the medicine albumin nanoparticles disclosed by the invention has the advantages that the preparation method is environmentally-friendly, organic solvents having medium and high toxins are not used, an emulsifying and volatilizing process is not included, besides, the solidification effect and the dispersion effects of the nanoparticles are completed at the same time, a few technology steps are used, and the time consumption is short. The particle diameter of the medicine albumin nanoparticles prepared by the method is generally smaller than 800nm.

Description

technical field [0001] The invention relates to a method for preparing pharmaceutical albumin nanoparticles, in particular to a method for preparing pharmaceutical albumin nanoparticles composed of albumin, a coagulant and a hydrophobic antitumor drug, and belongs to the technical field of pharmaceutical preparations. Background technique [0002] Cancer is a disease with a high mortality rate, which seriously threatens human health. Traditional anticancer drugs (such as taxanes, doxorubicin, camptothecin, and curcumin) have poor water solubility and membrane permeability, low bioavailability, and strong toxic and side effects. In order to improve the effectiveness of drugs and reduce side effects, nano-drug delivery systems have emerged. [0003] The nano drug delivery system is an important branch of the drug delivery system. The drug is loaded on the carrier, and the drug release in the body can be specifically changed by using its nano characteristics (small size, surfa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K31/704A61K45/00A61K47/42A61P35/00
CPCA61K9/5169A61K9/5192A61K31/704A61K45/00A61P35/00
Inventor 隋小宇韩翠艳刘婷婷钱佳怡潘虹
Owner QIQIHAR MEDICAL UNIVERSITY
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