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The New Application of Patchouli Essential Oil

A technology of patchouli, volatile oil, applied in the direction of applications, chemicals for biological control, fungicides, etc.

Active Publication Date: 2021-09-10
SHENZHEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no document disclosure related to the technical solution of the present invention

Method used

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  • The New Application of Patchouli Essential Oil
  • The New Application of Patchouli Essential Oil
  • The New Application of Patchouli Essential Oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the preparation of patchouli volatile oil

[0020] (1) Add 8 times the mass of water to 1000g of chopped patchouli, soak for 2 hours, and use steam to extract the volatile oil in patchouli by heating and distillation. ;

[0021] (2) The extracted fluid was dried with anhydrous sodium sulfate to remove the contained water to obtain the volatile oil of patchouli, which was put into a brown sample bottle, sealed, and stored at -20°C until use.

Embodiment 2

[0022] Embodiment 2: the activation of pathogenic bacteria

[0023] (1) Potato Dextrose Agar (PDA): 200g potato, 20g glucose, 20g agar, 1000mL distilled water;

[0024] (2) Purify and identify the pathogenic bacteria isolated from the roots and shoots of Panax notoginseng, and identify them as Fusarium solani, Pythium aphanidermatum, Cylindrocarpondestructans, Botrytis cinerea cinerea), Colletotrichum gloeosporioides and Rhizoctonia solani, the above strains were inoculated on PDA medium for activation, and after inoculation for 3 to 4 times, vigorously growing strains were taken for later use.

Embodiment 3

[0025] Embodiment 3: GC-MS measures the chemical composition of patchouli volatile oil

[0026] (1) GC conditions: Instrument model: Agilengt Technologies 7890B-5977B; Chromatographic column: HP-5MS30m×250μm×0.25μm;

[0027] (2) EI source ionization source temperature: 230°C; quadrupole temperature: 150°C; scanning range: 30-500m / z; inlet temperature: 285°C; split ratio 10:1; sample volume: 1μl; Energy: 70eV; temperature program of column oven: 50°C for 4min, 5°C / min to 120°C, 1°C / min to 180°C, 10°C / min to 280°C and hold for 16min; The retention time of the alkane continuous carbon (C9-C25) is calculated, and the retention time and mass spectrum of the compound are compared with the NIST17.L database to determine the final compound. The test results are shown in the table below;

[0028]

[0029]

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PUM

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Abstract

The invention discloses a new application of patchouli volatile oil in agriculture; that is, its application in the prevention and treatment of root rot of Chinese medicinal materials or crops, or its application in the prevention and treatment of pathogenic bacteria that cause diseases in the aboveground parts of Chinese medicinal materials or crops ; The experimental results showed that the volatile oil of Patchouli was effective against the 3 main pathogenic bacteria Fusarium solani, Pythium and Cylindrosporium destructor that caused the root rot of Panax notoginseng and the 3 pathogenic bacteria that caused the above-ground diseases of Panax notoginseng Botrytis cinerea, glenospora anthracnose, and rhizoctonia solani have a strong inhibitory effect on mycelium growth. When the concentration is 50mg / mL, the inhibition rate of three kinds of aboveground fungus patchouli oil reaches more than 80%. For some underground fungi, the volatile oil of Patchouli has the same inhibitory effect as hymexazol; due to the volatile nature of the volatile oil and its aromatic and anti-fouling characteristics, the chemical fertilizers or pesticides developed with it will have the advantages of safety, effectiveness, and low residue.

Description

technical field [0001] The invention relates to the application of the volatile oil of patchouli, in particular to the control effect of the volatile oil of patchouli on root rot of Chinese medicinal materials or crops and various diseases of the aboveground parts of Chinese medicinal materials or crops, and belongs to the technical field of biopesticides. Background technique [0002] Sanqi (Panax notoginseng (Burk.) F.H.Chen) is a perennial herbaceous plant of the Araliaceae ginseng genus. Its leaves, leaves and flowers can be used as medicine, and the range of medicine is very wide. The social demand is constantly increasing. With the rapid increase of the planting area of ​​Panax notoginseng, its disease problem is becoming more and more serious, which has become the main obstacle to the production of Panax notoginseng. At present, the prevention and treatment of Panax notoginseng disease mainly relies on various chemical agents. Although chemical pesticides are effectiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/22A01P3/00
CPCA01N65/00A01N65/22
Inventor 程永现董鲜
Owner SHENZHEN UNIV