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Bradyrhizobium sp. suitable for drug resistance, stress resistance and nitrogen fixation in Huanghuai region and application of Bradyrhizobium sp.

A technology of slow-growing rhizobia and microbial strains, applied in the field of microorganisms, can solve the problems of lack of rhizobia formulation and application technology, lack of application effect of rhizobia strains, restricting the application of rhizobia, etc., so as to increase the number of needles and reduce nitrogen fertilizer. the use of and the effect of reducing pollution

Active Publication Date: 2019-09-20
安徽微壤生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems are: the lack of adaptable and efficient nitrogen-fixing rhizobia strains, the lack of research on the application effect of rhizobia strains in different ecological environments, the lack of rhizobia formulations and application technologies suitable for the current planting methods, etc. These problems are serious Restricts the application of rhizobia

Method used

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  • Bradyrhizobium sp. suitable for drug resistance, stress resistance and nitrogen fixation in Huanghuai region and application of Bradyrhizobium sp.
  • Bradyrhizobium sp. suitable for drug resistance, stress resistance and nitrogen fixation in Huanghuai region and application of Bradyrhizobium sp.
  • Bradyrhizobium sp. suitable for drug resistance, stress resistance and nitrogen fixation in Huanghuai region and application of Bradyrhizobium sp.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1. Isolation and functional identification of Bradyrhizobium HHPB1

[0067] The present invention uses the peanut rhizobium isolated and identified in Tangshan, Hebei as materials, measures the nitrogen fixation ability and adaptability (drug resistance, drought resistance, salt-alkali resistance, etc.) Efficient nitrogen-fixing peanut rhizobia.

[0068] 1. Materials and methods

[0069] 1. Experimental materials

[0070] (1) strain

[0071] The bradyrhizobium was collected, classified and identified from Tangshan, Hebei in the early stage of our laboratory.

[0072] (2) Media and reagents used

[0073] ①YMA medium

[0074] Mannitol 10.0g, K 2 HPO 4 0.25g, KH 2 PO 4 0.25g, MgSO 4 0.1g, NaCl 0.1g, yeast extract 3.0g, agar 15g (plus solid medium), deionized water 1L, pH 6.8-7.0, sterilized at 121°C for 20min.

[0075] ②TY medium

[0076] Tryptone 5.0g, yeast extract 3.0g, CaCl 2 0.6g, 15g of agar (plus solid medium) 1L of deionized water, pH 6.8-7...

Embodiment 2

[0129] Embodiment 2, field application research of bradyrhizobium HHPB1

[0130] 1. Field application method of Bradyrhizobium HHPB1

[0131] 1. Strain activation and expansion culture: About 15 days before sowing, the Bradyrhizobium HHPB1 strain stored in the refrigerator at -80°C was activated on the YMA solid medium by three-section line method, and cultivated in a constant temperature incubator at 28°C for 7 days. Isolated single colonies. Use a sterile bamboo stick to pick a single colony into TY liquid medium for expansion culture, place it at 28°C, and shake it at 180 rpm until the bacterial liquid OD 600 =1.2, the seed solution was obtained. Then seed liquid is inoculated in the new TY liquid culture medium according to the ratio of 5% (volume fraction), cultivates 3-5 days at 28 ℃ of 180rpm incubators, obtains bacterial liquid (with the non-inoculated culture medium as blank control, bacterium Liquid concentration is about 1 x 10 10-11 cfu / ml).

[0132] 2. Before...

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Abstract

The invention discloses a Bradyrhizobium sp. suitable for drug resistance, stress resistance and nitrogen fixation in Huanghuai region and an application of the Bradyrhizobium sp.. The invention provides Bradyrhizobium sp. HHPB1, with the preservation number of CGMCC No.17501 in China General Microbiological Culture Collection Center. The Bradyrhizobium sp. HHPB1 has wide adaptability, such as drought resistance, pesticide resistance, acid resistance and the like, and is a biosafety strain. In Huanghuai region, fresh weight, nodule number and needle number of peanut in different development stages can be significantly increased, and the fruit saturation rate can be increased to a certain extent, with the highest peanut yield increased by 27.6%. The Bradyrhizobium sp. HHPB1 is a stable and efficient microbial fertilizer strain resource for reducing weight and increasing efficiency.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a drug-resistant, stress-resistant and nitrogen-fixing bradyrhizobium strain suitable for the Huanghuai region and its application. Background technique [0002] Nitrogen fertilizer is one of the essential fertilizers in crop production, especially legumes have a greater demand for nitrogen fertilizer. Peanut is an important grain and oil crop in my country's agricultural production, and the increase in its output is material input-dependent. my country's peanut production has always relied on a large amount of chemical fertilizer input, and excessive use of chemical fertilizers will not only cause a large amount of waste of resources but also cause a series of environmental problems, such as: damage to the soil and soil microbial community structure, groundwater pollution and greenhouse gas emissions. In order to develop green agriculture, reducing the use of chemical fertilizers ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C05F11/08A01C1/06C12R1/01
CPCA01C1/06A01N63/00C05F11/08C12N1/20C12N1/205C12R2001/01
Inventor 隋新华尚佼颖吴月霍斌李永华陈腊马钧
Owner 安徽微壤生物科技有限公司
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