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Preparation method of collagen peptide

A collagen peptide and substance technology, applied in the field of collagen peptide preparation, can solve the problems such as the degreasing rate and protein utilization rate need to be improved, solvent residue, low molecular weight of collagen peptide, etc., achieve controllable molecular weight distribution range, improve saltiness Taste and umami, high protein utilization effect

Active Publication Date: 2019-10-11
安徽盛美诺生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using acid or alkali treatment alone can easily lead to chemical denaturation of collagen; using enzyme treatment alone, although the preparation conditions are mild, but the degreasing rate is low; using solvent extraction alone can remove grease but it is difficult to remove impurity proteins, and the product There may be residual solvents in the
[0009] Chinese patent application CN 104561206A discloses an easily absorbable collagen peptide. In the preparation process, tilapia bone is used as raw material, degreased by chloroform and ethanol, and pepsin, papain and neutral protease are selected under acidic conditions. Through three-step enzymatic hydrolysis, the obtained collagen peptide has a low molecular weight and is easy to absorb, but the fat-removing rate and protein utilization rate need to be improved

Method used

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  • Preparation method of collagen peptide
  • Preparation method of collagen peptide
  • Preparation method of collagen peptide

Examples

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Effect test

Embodiment 1

[0062] A preparation method of collagen peptides, comprising the steps of:

[0063] (1) Cut the chicken feet with fingertips removed into 3-5cm sections, wash them with clean water, grind them into minced form with a grinder, and obtain substance A;

[0064] (2) Mix the mixed solvent of petroleum ether / absolute ethanol (volume ratio 1:0.5) and substance A according to the mass ratio of 1:1, mix at 10°C, and extract with ultrasonic assistance. The ultrasonic power is 100w, and the ultrasonic time is 5min. , filter, remove solvent, obtain substance B;

[0065] (3) Mix the alkaline lipase solution and substance B according to a mass ratio of 1:1, the mass fraction of the lipase in the alkaline lipase solution is 1%, and the concentration of the alkali is 0.01mol / L in terms of hydroxide ions , ultrasonic-assisted enzymatic hydrolysis, the ultrasonic power is 100w, the ultrasonic time is 20min, filtered, and the filtrate is washed with water until neutral to obtain substance C;

...

Embodiment 2

[0068] A preparation method of collagen peptides, comprising the steps of:

[0069] (1) Cut the chicken feet with fingertips removed into 3-5cm sections, wash them with clean water, grind them into minced form with a grinder, and obtain substance A;

[0070] (2) Mix the mixed solvent of petroleum ether / absolute ethanol (volume ratio 1:2) and substance A according to the mass ratio of 10:1, mix at 50°C, and perform ultrasonic-assisted extraction. The ultrasonic power is 500w, and the ultrasonic time is 60min. , filtered, the solvent was removed, and the filtrate was repeatedly treated once according to the above steps to obtain substance B;

[0071] (3) The alkaline lipase solution is mixed with substance B according to a mass ratio of 10:1, the mass fraction of the lipase in the alkaline lipase solution is 8%, and the concentration of the alkali is 1mol / L in terms of hydroxide ions, Ultrasound-assisted enzymolysis, the ultrasonic power is 500w, the ultrasonic time is 60min, f...

Embodiment 3

[0074] A preparation method of collagen peptides, comprising the steps of:

[0075] (1) Cut the chicken feet with fingertips removed into 3-5cm sections, wash them with clean water, grind them into minced form with a grinder, and obtain substance A;

[0076] (2) Mix the mixed solvent of petroleum ether / absolute ethanol (volume ratio 1:1) and substance A according to the mass ratio of 5:1, mix at 30°C, and perform ultrasonic-assisted extraction. The ultrasonic power is 300w, and the ultrasonic time is 30min. , filtered, the solvent was removed, and the filtrate was repeatedly processed 5 times according to the above steps to obtain substance B;

[0077] (3) Mix the alkaline lipase solution with substance B according to a mass ratio of 5:1, the mass fraction of the lipase in the alkaline lipase solution is 4%, and the concentration of the alkali is 0.5mol / L in terms of hydroxide ions , ultrasonic assisted enzymatic hydrolysis, the ultrasonic power is 300w, the ultrasonic time i...

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Abstract

The invention relates to the technical field of foods, in particular to a preparation method of a collagen peptide. The preparation method comprises the steps of ultrasonic assisted solvent extraction, ultrasonic assisted alkaline lipase enzymolysis and multi-step enzymolysis. The method has the advantages that the degreasing and hybrid protein removal efficiency is high, and the protein utilization rate exceeds 82%, the product is nourishing, and the saline taste and umami of food can be improved.

Description

technical field [0001] The invention relates to the technical field of food, in particular to a preparation method of collagen peptides. Background technique [0002] Peptide is a substance in which amino acids are connected by peptide bonds. It is a type of compound between amino acids and proteins, and it is the basis for proteins to have tens of millions of functions. Proteins are hydrolyzed by a variety of proteases in the human digestive system to form polypeptides, which are not only absorbed by the body in the form of amino acids, but more in the form of oligopeptides. [0003] Collagen contains a large amount of glycine, accounting for about 27% of the total amino acids, and the content of proline and hydroxyproline is also particularly high, accounting for 14%. These two are unique amino acids of collagen, while tryptophan, tyrosine The content of essential amino acids such as amino acid and methionine is low, so collagen is an incomplete protein. Collagen is a wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/14A23L33/18A61K8/65A61Q19/00
CPCA23L33/18A61K8/65A61K2800/10A61K2800/805A61K2800/82A61Q19/00C07K14/78C12P21/06
Inventor 谢建春谭佳王雅欣杜文斌
Owner 安徽盛美诺生物技术有限公司
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