A kind of Streptomyces albicans x-18 and the method for producing ε-polylysine by using the bacterium

A technology of polylysine and X-18, which is applied in the field of bioengineering, can solve the problem of low independent intellectual property rights, low production of ε-polylysine, and late. Jiangnan University, Tianjin University of Science and Technology, Nanjing University of Technology, and South China University of Technology University and Nankai University and other issues, to achieve the effect of increasing production

Active Publication Date: 2021-05-04
SHENZHEN INNOVATION CENT OF SMALL MOLECULE DRUG DISCOVERY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The research on ε-polylysine was carried out earlier and relatively mature in Japan, and many strains with independent intellectual property rights have been obtained; related research in my country started relatively late, Jiangnan University, Tianjin University of Science and Technology, Nanjing University of Technology, South China University of Technology Universities and Nankai University and other units have done a lot of research on the screening, mutagenesis, molecular improvement breeding and fermentation production of ε-polylysine-producing strains, but there are few strains with independent intellectual property rights; There are few reports on the increase of ε-polylysine production stimulated by drugs

Method used

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  • A kind of Streptomyces albicans x-18 and the method for producing ε-polylysine by using the bacterium
  • A kind of Streptomyces albicans x-18 and the method for producing ε-polylysine by using the bacterium
  • A kind of Streptomyces albicans x-18 and the method for producing ε-polylysine by using the bacterium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Describe how to screen and obtain the bacterial strain Streptomyces albicans ( Streptomyces albulus ) X-18

[0050] (1) Soil sample collection: Soil (soil samples were collected from Henan, Hebei, Jiangsu and other places) was sampled, and the soil at 5 cm below the surface of relatively dry and fertile soil was taken.

[0051] (2) Enrichment culture: 30 soil samples were collected, 2.5g of the samples were weighed and added to 25mL sterile enrichment medium, and incubated at 28°C and 150rpm for 2 days.

[0052] The composition of enrichment medium is (g / L): glycerol 10, agar 15, yeast extract 0.1, NaH 2 PO 4 0.68, MgSO 4 ·7H 2 O 0.25, (NH 4 ) 2 SO 4 0.66, 140mL trace mineral solution, pH 7.0, balance water.

[0053] The composition of trace mineral solution is (g / L): NaCl 1, FeSO 4 ·7H 2 O 0.1, ZnSO 4 ·7H 2 O 0.1, CaCl 2 2H 2 O 0.1, Alum 0.01, CuSO 4 ·5H 2 O 0.01, CoCl 2 ·6H 2 O 0.1, H 3 BO 3 0.01, NH 4 MO 2 4H 2 O 0.01, the balance is water. ...

Embodiment 2

[0057] Describe how to realize the Streptomyces albus ( Streptomyces albulus ) X-18

[0058] (1) Separation and purification of a single colony slant was submitted to Shanghai Bioengineering Co., Ltd. for sequencing, and the sequencing results were submitted to the NCBI database for comparison. The results showed that the 16S rDNA sequence homology between strain X-18 and Streptomyces albicans was as high as 98%. The phylogenetic tree of strain X-18 was constructed using MEGA 6.0 software image 3 , From the evolutionary distance analysis, it was found that X-18 was close to the standard type strain of Streptomyces albicans.

[0059] (2) According to the morphological characteristics, physiological and biochemical characteristics (see Table 1 below) and 16SrDNA characteristics of the strain X-18, the strain X-18 was determined to be Streptomyces albicans ( Streptomyces albulus ).

[0060]

[0061] Note: "+" indicates positive reaction; "-" indicates negative reaction. ...

Embodiment approach

[0065] Describe how to realize the raising of product epsilon-polylysine output, implementation method is as follows:

[0066] (1) Inclined solid culture

[0067] Inoculate the Streptomyces albicans CGMCC No. 17536 strain on a solid slant medium, and culture it at 26°C for 5 days to obtain the strains cultured on the slant solid, and set aside;

[0068] The formula of the solid slant medium is: for every 1000mL medium, add 2.0g of peptone, 7g of glucose, 1g of yeast powder and 15g of agar, pH 7.5, and the balance is water. ;

[0069] (2) Shake flask seed culture

[0070] Take the bacteria cultured on the slant solid prepared in the above step (1), add sterile water to make a bacterial suspension, and add 2 mL of sterile water according to one ring of bacteria; inoculate the bacterial suspension into the liquid seed medium , the inoculum volume was 5% of the volume of the liquid seed medium, the culture temperature was 26°C, the shaking table speed was 200 r / min, the pH was ...

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Abstract

The invention relates to a streptomyces albicans X-18 and a method for producing ε-polylysine by using the bacteria, belonging to the technical field of bioengineering. The Streptomyces albicans X‑18 is isolated and screened from soil, and has been preserved with the preservation number CGMCC No.17536. Utilize described Streptomyces albicans X-18 to ferment and produce ε-polylysine, and by improving the fermentation medium and fermentation conditions, in combination with the method of intermediate feeding and adding inducers, the fermentation of ε-polylysine The output can reach 3.5‑4.2g / L. The invention mainly uses strains with independent intellectual property rights, and uses cheap cornstarch as the main component of the fermentation medium, thereby reducing the production cost and increasing the fermentation output.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a streptococcus albicans X-18 and a method for producing ε-polylysine by using the bacteria. Background technique [0002] Food is extremely vulnerable to microbial attack during storage, and the mildew and spoilage caused by it have a great impact on human life. According to incomplete statistics, about 20% of the food in the world is wasted due to mildew and corruption, and it also endangers people's health. Traditional anti-corrosion methods such as drying and salting will greatly change the flavor of the processed food. Therefore, adding food preservatives is still one of the effective means of food processing and preservation. During the use of synthetic food preservatives, people have gradually discovered that they have a great impact on human health. In recent years, the research and use of natural preservatives has gradually attracted people's attent...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P13/02C12R1/47
CPCC12N1/20C12P13/02C12N1/205C12R2001/47
Inventor 王大红徐鹏符丹丹郑迎莹沈文浩王梦洋孙建瑞原江锋古绍彬
Owner SHENZHEN INNOVATION CENT OF SMALL MOLECULE DRUG DISCOVERY CO LTD
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