Method for stable and quick production of epsilon-polylysine

Abstract

The invention relates to a method for stable and quick production of epsilon-polylysine, and belongs to the technical field of fermentation engineering. The method for stable and quick production of the epsilon-polylysine comprises the steps that strain amylase is used for producing streptomycesdiastatochromogenes, and fermentation culture is carried out in a culture medium containing a suitable carbon source and nitrogen source. The method for stable and quick production of the epsilon-polylysine has the advantages that stable and quick fermentation production of the epsilon-polylysine is carried out by directly inoculating a spore suspension, taking a pH end point as a subcultivation index and adopting a one-step pH reduction strategy, so that the yield is increased by 80-130%, and the cycle is shortened by 28-45% compared with a conventional process; the steps of an existing production process are changed, thereby simplifying an existing fermentation production method, significantlyimproving the production intensity of the epsilon-polylysine, not only shortening the fermentation cycle and reducing the cost, but also reducing the discharge of fermentation waste liquid and exhaust gas, and reducing the environmental pollution, in addition, the process is simple and easy to enlarge, and industrialized and large-scale production is easy to achieve.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, in particular to a method for stably and rapidly producing ε-polylysine. Background technique [0002] ε-polylysine (also known as ε-poly-L-lysine, ε-Poly-L-lysine, ε-PL) is one of the two amino acid homopolymers found in nature. Metabolic synthesis (mainly actinomycetes, such as Streptomyces albicans, Streptomyces amylase chromogenes, Lispora, Streptomyces virginia, Streptomyces griseus, Streptomyces nonhygroscopic, in addition, Arthrobacter globosa and some Bacillus can also Synthetic) natural product, which consists of 25-35 α-carboxyl and ε-amino linked L-amino acid residues (L-Lys). [0003] ε-PL is a polycationic polypeptide with broad-spectrum antibacterial activity against microorganisms, including bacteria such as Gram-positive and Gram-negative bacteria, fungi, and certain viruses. The mouse feeding experiment showed that this polymer had almost no acute toxicity, sub...

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Problems solved by technology

(the literature source of above-mentioned fermentation method: P Kahar, T Iwata, J Hiraki, E YPark and M Okabe.Enhancement ofε-polylysine production by Streptomycesalbulus strain 410using pH control[J].Journal of Bioscience and Bioengineering, 2001,91 (2): 190-194. At present, most of them adopt the two-stage pH control in this document), but for this kind of fermentation method, the fermentation culture cycle is virtually increased, which is likely to cause low production efficiency of ε-PL, increase energy consumption, and the initial pH If it is too high, it will also increase the possibility of bacterial contamination. Therefore, finding a stable seed cultivation method and a rapid fermentation method is of great significance for breaking the existing technical barriers and improving the fermentation process.