Preparation method of amnion tissue engineering de-immunized skin scaffold

A tissue engineering and immunogenic technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of inconvenient storage, inability to use as needed, the introduction of immunogenicity of allogeneic cells, and difficulty in overcoming the amniotic membrane, etc. Large-scale preparation, wide-ranging effects
CN110404119AActive Publication Date: 2019-11-05上海越增生物科技有限公司
6 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
上海越增生物科技有限公司
Publication Date
2019-11-05

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a preparation method of an amnion tissue engineering de-immunized skin scaffold, comprising the following steps: rinsing and disinfecting amnion, then immersing in a commercialamnion cell culture medium, culturing in a CO2 incubator at 37 DEG C for 72 h, taking the cultured amnion out of the culture medium, centrifuging the cultured amnion and filtering through a 0.22 micron filter membrane and then sub-packaging, and cryopreserving in a refrigerator at 80 DEG C; carrying out continuous gradient dehydration on the amnion with ethanol for 3 days, aseptically air-drying,cutting into different specifications of amnion patches according to clinical requirements, and carrying out aseptic vacuum package for later use. The method adopted by the invention can realize thepurpose of simple, rapid, high-efficiency and large-scale preparation of medical artificial skin; the material used is human or pig amnion, has a wide source, is mostly waste after production and is low-cost, and there is no ethical problem. The invention has been verified on skin regeneration after burns in rats and pigs (large animal models), and the effect is remarkable and no adverse immune reaction is observed.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a preparation method of amnion tissue engineering deimmunized skin scaffold. Background technique

[0002] Amnion tissue discarded during childbirth is one of the important sources of seed cells for regenerative medicine. It has a wide range of sources, is easy to obtain, and has no ethical and moral disputes. It has become a research hotspot in recent years. Amnion, as an immune-exempt biological graft, has biological properties such as anti-inflammation, anti-adhesion, pain relief, and promotion of epithelialization. It has been applied to burn patients and other surgical operations as early as 1910 to promote epidermalization, Reduce pain and prevent infection. In recent years, with the further understanding of amniotic membrane and the development of amniotic membrane preservation and preparation technology, amniotic membrane has once again become a research and application hotspot, and is widely used in corneal reconstruc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More