Fresh-keeping agent for bacillus subtilis and preparation method thereof

A Bacillus subtilis and preservative technology is applied in the field of Bacillus subtilis preservative and its preparation, and achieves the effects of achieving freshness, reducing the risk of mildew, and prolonging the storage period

Inactive Publication Date: 2019-11-05
昆山兴嘉辰食品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on Bacillus subtilis as a fresh-keeping agent for fruits and vegetables

Method used

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  • Fresh-keeping agent for bacillus subtilis and preparation method thereof

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Embodiment 1

[0031] Prepare solid medium for Bacillus subtilis: beef extract 1.0-5.0g, peptone 5g, NACl4g, agar 10g, water 450ml, liquid medium: peptone 10g, potassium hydrogen phosphate 5g, magnesium sulfate 10g, glycerol 8g, water 1000ml, The prepared Bacillus subtilis strains were inoculated in solid medium. After overnight, the Bacillus subtilis on the solid medium was extracted and inoculated into the liquid medium. At 25°C and 165 r / min, keep the liquid medium inoculated with Bacillus subtilis in a shaking state for 12 hours. The liquid culture medium and the Bacillus subtilis in it are filtered, the bacterial cells are removed through the bacterial filter, and the filtrate is collected. The precipitated protein in the filtrate was extracted by gradient concentrations of ammonium sulfate, and the concentration difference of each gradient of ammonium sulfate was 10%. Centrifuge at 7500r / min and 5°C for 15min to further collect the precipitate, dissolve it in sodium phosphate saline ...

Embodiment 2

[0033] Prepare solid medium for Bacillus subtilis: beef extract 1.0-5.0g, peptone 10g, NACl7g, agar 15g, water 800ml, liquid medium: peptone 15g, potassium hydrogen phosphate 8g, magnesium sulfate 20g, glycerol 5g, water 1200ml, The prepared Bacillus subtilis strains were inoculated in solid medium. After overnight, the Bacillus subtilis on the solid medium was extracted and inoculated into the liquid medium. At 30°C and 170 r / min, the liquid medium inoculated with Bacillus subtilis was kept in a shaking state for 16 hours. The liquid culture medium and the Bacillus subtilis in it are filtered, the bacterial cells are removed through the bacterial filter, and the filtrate is collected. The precipitated protein in the filtrate was extracted by gradient concentrations of ammonium sulfate, and the concentration difference of each gradient of ammonium sulfate was 10%. The precipitate was further collected by centrifugation at 8000r / min and 6°C for 20min, and dissolved in a sodiu...

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Abstract

The invention provides a fresh-keeping agent for bacillus subtilis and a preparation method thereof, wherein the preparation method comprises the following steps: S1, preparing a culture medium, and inoculating a solid culture medium with a prepared bacillus subtilis strain; S2, extracting bacillus subtilis on the solid culture medium after overnight, and inoculating into a liquid culture medium;S3, keeping the liquid culture medium inoculated with bacillus subtilis in a state of oscillation; S4, after overnight, filtering the liquid culture medium and bacillus subtilis in the liquid culturemedium, removing the bacteria through a bacterial filter, and collecting a filtrate; S5, extracting a precipitated protein in the filtrate by a fractional precipitation method; and S6, furthermore collecting a precipitate under a centrifugal condition, dissolving in a buffer solution, carrying out dialysis treatment on the buffer solution, and desalting to obtain an antibacterial protein. The fresh-keeping agent for bacillus subtilis can realize bacteriostasis of fruit and vegetable pathogenic bacteria, thereby realizing fresh keeping of fruits and vegetables, prolonging the storage period ofthe fruits and vegetables, and facilitating the transportation and storage of the fruits and vegetables.

Description

technical field [0001] The invention relates to the technical field of application of bacillus subtilis, in particular to a preservative for bacillus subtilis and a preparation method thereof. Background technique [0002] Bacillus subtilis (Bacillus subtilis) is a kind of Gram-positive bacteria with aerobic endophytic spores. It is a non-toxic and harmless probiotic to humans and animals. Significant antibacterial activity and stress resistance, has been applied in agriculture. Bacillus subtilis grows fast, has simple nutrition, and can produce heat-resistant and stress-resistant spores. A lot of research has been done on the prevention and control of plant diseases. Some subtilis spores can show obvious effects on disease prevention and control of field corn, wheat, pepper, cucumber, etc. before harvest. However, there are few reports on the use of Bacillus subtilis as a fresh-keeping agent for fruits and vegetables. Contents of the invention [0003] The purpose of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30A23B7/155C12R1/125
CPCC07K1/36C07K1/34C07K1/30A23B7/155
Inventor 范俊峰
Owner 昆山兴嘉辰食品科技有限公司
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