A method for preparing drug-loaded ethosome microspheres capable of targeting dendritic cells by electrostatic spraying
A technology of dendritic cells and electrostatic spraying, which is applied in the direction of pharmaceutical formulations, liposome delivery, medical preparations of non-active ingredients, etc., can solve problems such as poor effect and harm to human health, and achieve short preparation time and stable Good performance, good for mass production
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Embodiment 1
[0030] (1) lecithin, cholesterol, octadecylamine are mixed in 30% (v / v) ethanol aqueous solution with the ratio of mass ratio 25:2.5:1, rotary evaporation obtains one deck liposome film; TRP 2 Peptide was added at 0.5 mg / ml to 30% (v / v) aqueous ethanol to obtain TRP 2 Peptide solution; add liposome membrane to TRP 2 Peptide solution, stirred to obtain loaded TRP 2 Ethosomes of peptides; will load TRP 2 Peptide ethosomes were added dropwise to the mannosylated polyethyleneimine solution, dispersed ultrasonically while stirring, and then immunoadjuvant drugs were added to obtain the drug-loaded ethosomes modified by mannosylated polyethyleneimine;
[0031] The preparation method of mannosylated polyethyleneimine is as follows: mixing 4-isosulfuric acid phenyl-A-D-mannoside and polyethyleneimine, adding methanol to initiate the reaction for 3 hours, then rotary evaporation, and freeze-drying for 3 days to obtain Mannosylated polyethyleneimine; wherein the mass ratio of 4-isosu...
Embodiment 2
[0038] (1) Lecithin, cholesterol, octadecylamine are mixed in 30% (v / v) ethanol aqueous solution with the ratio of mass ratio 25:2.5:1, rotary evaporation obtains one deck liposome film; Adriamycin hydrochloride (Dox) add 30% (v / v) ethanol aqueous solution with 0.5mg / ml, obtain Dox solution; Liposome membrane is joined in the Dox solution, stirs to obtain the ethosome of loading Dox; The ethosome of loading Dox Add it dropwise into the mannosylated polyethyleneimine solution, disperse it ultrasonically while stirring, and then add the EGFP gene expression plasmid (pEGFP) to obtain the drug-loaded ethosome modified by mannosylated polyethyleneimine;
[0039] The preparation method of mannosylated polyethyleneimine is as follows: mixing 4-isosulfuric acid phenyl-A-D-mannoside and polyethyleneimine, adding methanol to initiate the reaction for 3 hours, then rotary evaporation, and freeze-drying for 3 days to obtain Mannosylated polyethyleneimine; wherein the mass ratio of 4-isosu...
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