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Polygonum viviparum extract and preparation method and application thereof

The technology of Polygonum vulgaris and its extract is applied in the field of preparation of Polygonum vulgaris extract, can solve the problems of residual toxic organic reagents, large dosage of traditional Chinese medicines, and few plant species, and achieves good inhibitory effect, obvious inhibitory effect, and good inhibitory effect. Effect

Active Publication Date: 2019-11-08
DONGGUAN HEC CORDYCEPS R&D CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although it is found in the prior art that Polygonum viburnum has natural anti-tumor substances and antioxidant substances such as flavonoids, phenols, and terpenes, it is well known that many types of medicinal plants contain these natural products, which can really eventually become antioxidant, There are very few types of plants for anti-tumor or tumor-treating drugs, and because the dosage of traditional Chinese medicine is too large compared with chemotherapy drugs, there are also problems of toxic organic reagents remaining, so it is generally used as an adjuvant treatment for cancer treatment
Therefore, it is necessary to find Chinese medicines and drugs that can be truly superior to cisplatin in efficacy and safety as first-line drugs for cancers such as small cell and non-small cell lung cancer, head and neck tumors, bladder cancer, testicular cancer, and ovarian cancer. Its extraction is a very great challenge

Method used

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  • Polygonum viviparum extract and preparation method and application thereof
  • Polygonum viviparum extract and preparation method and application thereof
  • Polygonum viviparum extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Example 1: Preparation of lyophilized product of Polygonum viviparum fruit extract eluate

[0093] The dried Polygonum viviparum fruit medicinal material is crushed, passed through a No. 3 sieve, and 20 g is taken, and a 70% ethanol solution is used as the extraction solvent. The ratio of the volume of the extraction solvent to the mass of the raw material of the Polygonum viviparum fruit is 20 mL / g. The temperature was raised to the reflux of the reaction solution for extraction, and the extraction time was 4 hours to obtain the extract. Use a rotary evaporator to concentrate the extract at 60°C and transfer it to an evaporating dish in a 100°C water bath to evaporate to dryness to obtain an extract. Take the extract and dissolve it in 50% ethanol solution to obtain a solution with a concentration of 80 mg / mL. The column is packed with D101 macroporous adsorption resin wet method, and the sample is loaded at a flow rate of 3.6 BV / h and adsorbed for 2 hours. Then, at a fl...

Embodiment 2

[0094] Example 2: Anti-tumor activity test of lyophilized product of Polygonum viviparum fruit extract eluate

[0095] Instrument: Ultra-micro spectrophotometer (BMG LABTECH), CO 2 Constant temperature incubator (Thermo Fisher Scientific), 96-well plate (Guangzhou Jiete Biological Filtration Co., Ltd.).

[0096] Reagents: 0.25% pancreatin-EDTA (1X) (gibco), penicillin streptomycin mixture (hyclone), ethanol (Xilong Science Co., Ltd.), RPMI1640 medium (hyclone), DMEM medium (hyclone), CCK -8 kit (Dongren Chemical Technology Co., Ltd.), PBS phosphate buffer (Beijing Dingguo Changsheng Biotechnology Co., Ltd.), EDTA (sigma), fetal bovine serum (gibco), cell lines were purchased from ATCC.

[0097] Cell culture: use RPMI1640 or DMEM complete medium (1640 / DMEM+10% fetal bovine serum+1% penicillin streptomycin mixture) culture, place at 37℃, 5% CO 2 Cultivate in an incubator. Observe the cells with an inverted microscope once a day, and change the culture medium every 2 days. In the cult...

Embodiment 3

[0111] Example 3: Antioxidant activity test of the lyophilized product of Polygonum viviparum fruit extract eluate

[0112] Preparation of DPPH solution: accurately weigh 24 mg of DPPH, dissolve it with 95% ethanol and prepare a 0.24 mg / mL solution, and store it at 0-4°C in the dark.

[0113] Preparation of DPPH ethanol solution: 0.1mL 95% ethanol and 3mL 0.24mg / mL DPPH solution were added to the same test tube, shaken, placed in the dark for 30 minutes, with absolute ethanol as a blank, the absorbance Ac was measured at 517nm wavelength.

[0114] Preparation of sample solution: Prepare the concentration according to the following table, the sample is prepared with 95% ethanol, and the concentration is shown in Table 3.

[0115] Table 3: Sample solution concentration information table

[0116]

[0117]

[0118] Shake the sample solutions in Table 3, ultrapure water, DPPH ethanol solution, and 95% ethanol, respectively, and let stand for 45 minutes in the dark at room temperature.

[0119...

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Abstract

The invention belongs to the field of Chinese herbal medicine extraction, provides a polygonum viviparum extract and a preparation method and application thereof, in particular, provides the preparation method of the polygonum viviparum extract and the polygonum viviparum exact with high anti-tumor activity prepared by the preparation method; and the invention further provides the application of the polygonum viviparum extract in the preparation of anti-tumor drugs.

Description

Technical field [0001] The invention belongs to the field of Chinese medicinal material extraction, and specifically relates to the preparation and application of Polygonum vulgare extract. Background technique [0002] Cancer, that is, malignant tumor, has many types. Among them, the incidence of breast cancer, lung cancer, and blood cancer is extremely high, which seriously affects human health. Breast cancer is the number one killer of female cancer deaths. Lung cancer is the first malignant tumor in both male morbidity and mortality, female morbidity second, and mortality second. Melanoma is a pigmented nevus with malignant changes. Although the incidence is low, the degree of malignancy is high, metastasis occurs early, and the mortality rate is high. It needs timely treatment. Lymphoma is a systemic disease due to the distribution characteristics of the lymphatic system, which can invade almost any tissue and organ in the body, and its mortality rate accounts for 11-13th...

Claims

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Application Information

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IPC IPC(8): A61K36/704A61P35/00A61P39/06
CPCA61K36/704A61K2236/333A61K2236/51A61K2236/55A61P35/00A61P39/06
Inventor 钱正明甄达明何焯斌李春红李文佳谢俊杰章云飞许明龙
Owner DONGGUAN HEC CORDYCEPS R&D CO LTD
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