A kind of determination method of biotin content in eggs

A determination method and technology of biotin, which is applied in the field of determination of biotin content in eggs, can solve the problems of complex matrix, difficulty in accurate determination, low biotin content in eggs, etc., and achieve the effects of reducing pollution, high accuracy and high sensitivity

Active Publication Date: 2022-02-18
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a method for determining the content of biotin in eggs to solve the problems that the content of biotin in eggs is low, the matrix is ​​complicated, and it is difficult to measure accurately

Method used

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  • A kind of determination method of biotin content in eggs
  • A kind of determination method of biotin content in eggs
  • A kind of determination method of biotin content in eggs

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Experimental program
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Embodiment

[0032] 1 Instruments, reagents and materials

[0033] ACQUITYTM ultra-high performance liquid chromatography and Xevo TQ-S mass spectrometer equipped with electrospray (ESI) ionization source and MasslynxTM chromatographic workstation (Waters, USA); ultrasonic cleaner (Ningbo Xinzhi Biotechnology Co., Ltd.); MS3 vortex mixing N-EVAP-45 position nitrogen blowing instrument (Organomation, USA); SQP-electronic balance (Sartorius Scientific Instrument Co., Ltd.); Milli-Q ultrapure water system (Millipore, USA).

[0034] D-biotin standard (Dr.Ehrenstorfer, Germany); biotin-d2 is deuterated biotin isotope (ISOREAG); egg samples were purchased from local supermarkets. Methanol, acetonitrile (chromatographically pure, Fisher Company of the United States); formic acid (chromatographically pure, Sigma-Aldrich Company of the United States); QASIS PRiME HLB solid-phase extraction column (60mg, 3mL, Waters Company of the United States); sulfuric acid, acetic acid (excellent grade, Sinophar...

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Abstract

The invention discloses a method for determining the content of biotin in eggs, establishes an isotope dilution ultra-high liquid chromatography-tandem mass spectrometry method for determining the content of biotin in eggs, and uses a solid-phase extraction column to effectively remove impurities such as fat and phospholipids in eggs , effectively reducing the matrix effect, prolonging the service life of the chromatographic column, and reducing the pollution of the mass spectrometer. The addition of the isotope internal standard effectively corrects the influence of the matrix effect during the experiment and the deviation caused by the loss of biotin during the pretreatment process, and improves the accuracy and stability of the analytical method. The method has the advantages of short inspection cycle, high sensitivity, high accuracy and good precision. This method provides technical support for the accurate assessment of biotin content in eggs.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for determining biotin content in eggs. Background technique [0002] Biotin, also known as vitamin B7 and vitamin H, is a water-soluble vitamin that is commonly found in animals and plants, especially in animal liver tissue and egg yolk. As a cofactor of carboxylation, decarboxylation and dehydrogenation enzyme systems, biotin participates in the metabolism of proteins, fats and sugars, and is one of the vitamins necessary for animals to maintain normal physiological functions. Biotin often exists in a free state or in a state bound to proteins in the cells of animals. It is a ureido ring containing a sulfur atom and a side chain of valeric acid. There are currently 8 known isomers. The only naturally occurring There is D-biotin active. [0003] Eggs have comprehensive nutritional components, and the content of various nutritional components such as protein, fa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 卢兰香刘艳明薛霞魏莉莉公丕学武传香丁一王骏祝建华
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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