Dibromofluorescein derivative as well as synthetic method and application thereof

The invention discloses a dibromofluorescein derivative as well as a synthetic method of the derivative and an application of the derivative in detecting Al<3+>, and belongs to the technical field ofdetection of fluorescein derivatives and aluminum ions. The synthetic method of the dibromofluorescein derivative comprises the following steps: 1) dissolving hydrazine hydrate and dibromofluoresceininto methanol, and performing heating reflux to obtain a crude product; and performing rotary evaporation on the crude product, performing vacuum drying, and performing recrystallization by using methanol to obtain dibromofluorescein hydrazide; and 2) dissolving the dibromofluorescein hydrazide and 2-hydroxy-5-nitrobenzaldehyde into an absolute ethanol solution, performing heating reflux to obtaina crude product, performing rotary evaporation on the crude product, performing vacuum drying, performing recrystallization by using methanol, and performing drying to obtain the off-white solid powder 5-nitrosalicylaldehyde dibromofluorescein hydrazone (NDH). The invention also provides a method for detecting the aluminum ions. The method comprises the following step of quantitatively detectingthe content of the Al<3+> in a THF:H2O (9:1, v/v) solution by using the dibromofluorescein derivative as a probe. The detection method provided by the invention can detect the aluminum ions with highselectivity and high sensitivity, is simple and convenient to operate, and can be applied to cell imaging.