Poly-valerolactone type amphiphilic polymer based on tetraphenyl ethylene as well as preparation method and application of poly-valerolactone type amphiphilic polymer

A technology of polyvalerolactone and tetraphenylethylene, which is applied in the field of polymer design and preparation, and can solve the problems of great difference in transfection efficiency and single function
CN110527072AActive Publication Date: 2019-12-03BEIJING NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING NORMAL UNIVERSITY
Publication Date
2019-12-03

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Abstract

The invention discloses a poly-valerolactone type amphiphilic polymer based on tetraphenyl ethylene as well as a preparation method and an application of the poly-valerolactone type amphiphilic polymer. The disclosed compound is mainly prepared through a Michael reaction, an ROP reaction and a thio-ene click reaction, and the structure of the compound is confirmed by nuclear magnetism and mass spectrum. The poly-valerolactone type amphiphilic polymer has an aggregation-induced emission (AIE) effect, gel electrophoresis, dynamic light scattering, SEM and other test means prove that the polymercan aggregate DNA, and the diameter of formed nanoparticles is smaller than 200 nm. In-vitro transfection experiments prove that liposome formed by the poly-valerolactone type amphiphilic polymer anddioleoyl phosphatidyl ethanolamine (DOPE) can be used as a non-virogene vector.
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Description

technical field

[0001] The invention relates to the design and preparation of polymers, in particular to a tetraphenylethylene-based polyvalerolactone-type amphiphilic polymer and its preparation method and application. Background technique

[0002] Gene therapy has received a lot of attention in diseases such as genetic defects and cancer. Because both DNA and cell membrane are negatively charged, there is electrostatic repulsion, and there are nucleolytic enzymes in the cytoplasm, it is very difficult for naked DNA to be taken up by cells. Therefore, it is particularly important to develop a safe and effective gene transport tool, that is, a gene carrier. At present, non-viral gene vectors have received more attention than viral vectors. Non-viral vectors include liposomes, polymers, nanoparticles, and the like. Cationic polymers have the advantages of good biocompatibility and safety, easy modification and large-scale production. However, it is limited by low transfec...

Claims

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