Preparation method and culture medium of dibasic acid diaminopentane salt

The technology of dibasic acid pentamethylene diamine salt and dibasic acid ammonium salt is applied in the field of environment-friendly medium, which can solve problems such as pressure, and achieve the advantages of avoiding treatment process, reducing subsequent treatment work and cost, and reducing environmental damage. Effect

Inactive Publication Date: 2019-12-06
CATHAY R&D CENT CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that a large amount of inorganic salts will be produced in the process of preparing lysine by biological fermentation and then obtaining pentamethylenediamine, which will put a lot of pressure on the subsequent treatment, the invention provides a variety of environmentally friendly Culture medium and method for preparing dibasic acid pentamethylenediamine salt through fermentation and enzyme conversion process using the medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0085] Mainly use three kinds of medium in the specific embodiment:

[0086] 1. Seed medium

[0087] Contains the following ingredients: KH 2 PO 4 0.2-0.5%, MgSO 4 ·7H 2 O 0.15-0.45%, MnSO 4 ·H 2 O 10-20ppm, dibasic acid ammonium salt 0.1%-2.5%, glucose 5%-15%, corn steep liquor 0.05%-0.30%, threonine 0.015-0.045%, leucine 0.010-0.025%;

[0088] 2. Fermentation medium

[0089] Contains the following ingredients: KH 2 PO 4 0.02%-0.06%, MgSO 4 ·7H 2 O 0.10%-0.50%, MnSO 4 ·H 2 O0.010%-0.050%, dibasic acid ammonium salt 0.1%-1.0%, glucose 1.0%-6.0%, corn steep liquor 0.10%-0.50%, threonine 0.010%-0.030%;

[0090] 3. Feeding medium:

[0091] The following ingredients are included: glucose 10%-80%, dibasic acid ammonium salt 5%-60%.

[0092] Fermentation - enzymatic conversion method:

[0093] (1) Seed tank cultivation: 10L fermenter (working volume 4-7L), inoculum ratio 0.5-2%, aeration ratio 0.4-0.8vvm, temperature 35-39°C, speed 300-800rpm, tank pressure 0.05-0....

preparation Embodiment 1

[0100] Preparation of ammonium salt of dodecanedibasic acid

[0101] 1. Strain activation:

[0102] Take the glycerol tube strain of Candida and inoculate it into a seed bottle equipped with YPD culture, at natural pH, at 29°C, 220rpm, amplitude 26-50mm shaker culture for 1 day; YPD medium includes: 20g / L glucose, 10g / L yeast extract powder, 20g / l peptone; pH7.0-7.5;

[0103] 2. Cultivate in seed tanks and prepare seed solution:

[0104] Take the seeds from the seed bottle and put them into the seed tank with the seed medium. The initial pH value of the fermentation system after inoculation is 6.0. At 29°C, the ventilation ratio is 0.5vvm, and the tank pressure is 0.1MPa. The dissolved oxygen in the cultivation process is greater than or equal to 10%, cultivated for 18 hours, and the standard for cultivating mature seeds is OD after 30 times dilution 620 is 15;

[0105] Seed medium includes: 20g / L sucrose, 8g / L corn syrup, 5g / L yeast extract, 8g / L KH 2 PO 4 , 3g / L urea,...

preparation Embodiment 2

[0111] Preparation of ammonium sebacate

[0112] 1. Strain activation:

[0113] Take the glycerol tube strain of Candida and inoculate it into the seed bottle with YPD culture, the pH is natural, at 29°C, 220rpm shaker culture for 1 day; YPD medium includes: 20g / L glucose, 10g / L yeast extract powder , 20g / l peptone; pH7.0;

[0114] 2. Cultivate in seed tanks and prepare seed solution:

[0115] Take the seeds from the seed bottle and put them into the seed tank with the seed medium. The initial pH value of the fermentation system after inoculation is 6.0. At 29°C, the ventilation ratio is 0.5vvm, and the tank pressure is 0.1MPa. The dissolved oxygen in the cultivation process is greater than or equal to 10%, cultivated for 18-20h, and the standard for cultivating mature seeds is OD after 30 times dilution 620 is 15;

[0116] Seed medium includes: 20g / L sucrose, 8g / L corn syrup, 5g / L yeast extract, 8g / L KH2 PO 4 , 3g / L urea, prepared with water, and sterilized at 121°C for ...

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PUM

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Abstract

The invention discloses a preparation method of dibasic acid diaminopentane salt. The method comprises the following steps of: performing fermentation to generate lysine; mixing fermentation liquor ofthe lysine with lysine decarboxylase; carrying out decarboxylation reaction to generate diaminopentane; and adding a fermentation culture medium in the process of fermenting the lysine. The culture medium takes dibasic acid ammonium salt as a nitrogen source and also takes the dibasic acid ammonium salt as a substrate, and completes a lysine fermentation system in a mode of a supplemented culturemedium. The method for preparing the dibasic acid diaminopentane salt avoids the problem that a large amount of inorganic ions are generated in the process of preparing diaminopentane by prior biological fermentation methods, thereby avoiding the subsequent process for treating the inorganic ions, greatly reducing the subsequent treatment work and cost and reducing the damage of the inorganic ions to the environment.

Description

technical field [0001] The invention relates to an environment-friendly culture medium and a method for preparing dibasic acid pentamethylenediamine salt by fermentation of the culture medium. Background technique [0002] Diaminopentane (DN5), also known as cadaverine, is widely used in agriculture, medicine and industry. In agriculture, exogenous application of pentamethylenediamine can improve fruit setting and promote fruit ripening; in medicine, pentamethylenediamine can be used as an effective drug for treating dysentery; in industry, pentamethylenediamine prepared by biological fermentation can replace Diamines from petrochemical products undergo polymerization reactions to produce various polymers with different properties, thereby avoiding the disadvantages of high cost of petrochemical products and adverse effects on the environment, and can be widely used in aerospace, automotive parts, machinery Parts, electronic appliances and packaging materials and other fiel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12R1/72
CPCC12P13/001
Inventor 路飞徐敏于丽珺刘修才
Owner CATHAY R&D CENT CO LTD
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