Preparation method of small-molecule acanthopanax trifoliatus extract and application thereof
An extract and small molecule technology, applied in the field of plant application research, can solve problems such as poor absorption and loss of active ingredients, and achieve the effect of improving absorption rate and reducing loss
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Embodiment 1
[0050] A preparation method of small molecular bok choy extract, which comprises the following steps:
[0051] S1 rough extraction of bok choy extract, extracting active ingredients from fresh bok choy to obtain crude bok choy extract;
[0052] S2 Extremely quick freezing and thawing, quickly cool the crude bougainvillea extract to below zero, and freeze and thaw for 15 minutes;
[0053] S3 enzymatic hydrolysis, thawing the crude bok choy extract after freezing and thawing and raising the temperature to 15°C, adding salicylic acid and citric acid with a mass ratio of 1:2, the concentration of salicylic acid was 5%, and the concentration of citric acid was 8%, adjust it to an acidic environment, the pH is 4.5-5.5, add a compound enzyme composed of protease, peptidase and maltase with a mass ratio of 3:2:2 for enzymolysis, stir slowly during the enzymolysis process, stir The speed is 50rpm, and the time is 15 hours, and the bok choy enzymatic solution is obtained;
[0054] S4 ...
Embodiment 2
[0057] A preparation method of small molecular bok choy extract, which comprises the following steps:
[0058] S1 rough extraction of bok choy extract, extracting active ingredients from fresh bok choy to obtain crude bok choy extract;
[0059] S2 Extremely quick freezing and thawing, quickly cool the crude bougainvillea extract to below zero, freeze and thaw for 22Min;
[0060] S3 enzymatic hydrolysis, thawing the crude bok choy extract after freezing and thawing and raising the temperature to 18°C, adding salicylic acid and citric acid with a mass ratio of 1:3, the concentration of salicylic acid was 5%, and the concentration of citric acid was 8%, adjust it to an acidic environment, the pH is 4.5-5.5, add a compound enzyme composed of protease, peptidase and maltase with a mass ratio of 4:3:2 for enzymolysis, stir slowly during the enzymolysis process, stir The speed is 80rpm, and the time is 12 hours to obtain the bok choy enzymatic solution;
[0061] S4 acid-base neutra...
Embodiment 3
[0064] A preparation method of small molecular bok choy extract, which comprises the following steps:
[0065] S1 rough extraction of bok choy extract, extracting active ingredients from fresh bok choy to obtain crude bok choy extract;
[0066] S2 Extremely quick freezing and thawing, quickly cool the crude bougainvillea extract to below zero, and freeze and thaw for 30 minutes;
[0067] S3 enzymatic hydrolysis, thawing the crude bok choy extract after freezing and thawing and raising the temperature to 20°C, adding salicylic acid and citric acid with a mass ratio of 1:4, the concentration of salicylic acid was 5%, and the concentration of citric acid was 8%, adjust it to an acidic environment, the pH is 4.5-5.5, add a compound enzyme composed of protease, peptidase and maltase with a mass ratio of 5:4:2 for enzymolysis, stir slowly during the enzymolysis process, stir The speed is 100rpm, and the time is 10 hours, and the bok choy enzymatic solution is obtained;
[0068] S4...
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