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Preparation method of small-molecule acanthopanax trifoliatus extract and application thereof

An extract and small molecule technology, applied in the field of plant application research, can solve problems such as poor absorption and loss of active ingredients, and achieve the effect of improving absorption rate and reducing loss

Inactive Publication Date: 2019-12-13
ENPING JIAXIN DAILY NECESSITIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, bougainvillea extract has been applied to fields such as cosmetics, health products and food, but directly adding bougainvillea extract, because it contains more macromolecular substances (proteins, polypeptides), this will lead to poor absorption. Good, a lot of active ingredients are lost, how to transform it with small molecules is a topic worth studying

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A preparation method of small molecular bok choy extract, which comprises the following steps:

[0051] S1 rough extraction of bok choy extract, extracting active ingredients from fresh bok choy to obtain crude bok choy extract;

[0052] S2 Extremely quick freezing and thawing, quickly cool the crude bougainvillea extract to below zero, and freeze and thaw for 15 minutes;

[0053] S3 enzymatic hydrolysis, thawing the crude bok choy extract after freezing and thawing and raising the temperature to 15°C, adding salicylic acid and citric acid with a mass ratio of 1:2, the concentration of salicylic acid was 5%, and the concentration of citric acid was 8%, adjust it to an acidic environment, the pH is 4.5-5.5, add a compound enzyme composed of protease, peptidase and maltase with a mass ratio of 3:2:2 for enzymolysis, stir slowly during the enzymolysis process, stir The speed is 50rpm, and the time is 15 hours, and the bok choy enzymatic solution is obtained;

[0054] S4 ...

Embodiment 2

[0057] A preparation method of small molecular bok choy extract, which comprises the following steps:

[0058] S1 rough extraction of bok choy extract, extracting active ingredients from fresh bok choy to obtain crude bok choy extract;

[0059] S2 Extremely quick freezing and thawing, quickly cool the crude bougainvillea extract to below zero, freeze and thaw for 22Min;

[0060] S3 enzymatic hydrolysis, thawing the crude bok choy extract after freezing and thawing and raising the temperature to 18°C, adding salicylic acid and citric acid with a mass ratio of 1:3, the concentration of salicylic acid was 5%, and the concentration of citric acid was 8%, adjust it to an acidic environment, the pH is 4.5-5.5, add a compound enzyme composed of protease, peptidase and maltase with a mass ratio of 4:3:2 for enzymolysis, stir slowly during the enzymolysis process, stir The speed is 80rpm, and the time is 12 hours to obtain the bok choy enzymatic solution;

[0061] S4 acid-base neutra...

Embodiment 3

[0064] A preparation method of small molecular bok choy extract, which comprises the following steps:

[0065] S1 rough extraction of bok choy extract, extracting active ingredients from fresh bok choy to obtain crude bok choy extract;

[0066] S2 Extremely quick freezing and thawing, quickly cool the crude bougainvillea extract to below zero, and freeze and thaw for 30 minutes;

[0067] S3 enzymatic hydrolysis, thawing the crude bok choy extract after freezing and thawing and raising the temperature to 20°C, adding salicylic acid and citric acid with a mass ratio of 1:4, the concentration of salicylic acid was 5%, and the concentration of citric acid was 8%, adjust it to an acidic environment, the pH is 4.5-5.5, add a compound enzyme composed of protease, peptidase and maltase with a mass ratio of 5:4:2 for enzymolysis, stir slowly during the enzymolysis process, stir The speed is 100rpm, and the time is 10 hours, and the bok choy enzymatic solution is obtained;

[0068] S4...

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PUM

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Abstract

The invention relates to the field of plant application and research, in particular to a preparation method of a small-molecule acanthopanax trifoliatus extract and application thereof. The method comprises the following steps: firstly, extracting an acanthopanax trifoliatus extract; performing small molecule transformation on the acanthopanax trifoliatus extract by utilizing enzymatic hydrolysisreaction to ensure that small molecule substances which are difficult to absorb are transformed into small molecule substances which are easy to absorb; and finally, performing filtration, sterilization and filling, wherein active ingredients of the acanthopanax trifoliatus have the activities such as oxidation resistance, bacteria resistance, aging resistance and tumor resistance; performing enzymatic hydrolysis on proteins, polypeptide and saccharide through modification of small molecules to form the small molecule substances, so that the small molecule substances are absorbed by skin moreeasily, absorption ratios of the active ingredients of the acanthopanax trifoliatus are obvously improved, and loss of the active ingredients is reduced; performing enzymatic hydrolysis at a low temperature, and controlling enzymatic hydrolysis speed to ensure that the other active ingredients are not destroyed while the enzymatic hydrolysis is carried out at the same time; performing neutralization reaction to obtain soldium salicylate and sodium citrate, wherein the soldium salicylate and the sodium citrate can also be substances effective to a human body, and the phenomenon that the soldiumsalicylate and the sodium citrate are absorbed and utilized by the human body is not affected; and finally, filtering off added compound enzyme and not remaining the compound enzyme in a finished product.

Description

technical field [0001] The invention relates to the field of plant application research, in particular to a preparation method and application of a small molecular bok choy extract. Background technique [0002] In recent years, researchers have carried out in-depth research on the chemical constituents of bougainvillea. At present, many domestic and foreign scholars have isolated more than 30 kinds of monomeric compounds from the stems and leaves of bougainvillea, including polysaccharides, volatile oils, acids, flavonoids, saponins, and proteins, vitamins, iron, zinc, etc. Nutrients. [0003] A bougainvillea extract and its preparation method and application are disclosed in the Chinese patent library (CN201210274788.6). First, the whole bougainvillea plant is weighed, soaked in ethanol, and concentrated by rotary evaporation to obtain an aqueous solution, and then respectively use oily ether, Ethyl acetate and n-butanol extract the aqueous solution in sequence; the extr...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61Q19/02A61Q19/08A61K36/254A61P39/06A61P31/04A61P35/00A23L33/105
CPCA61K8/9789A61Q19/02A61Q19/08A61K36/254A61P39/06A61P31/04A61P35/00A23L33/105A61K2800/805A61K2800/82A61K2236/19A23V2002/00A23V2200/308A23V2200/318A23V2200/30A23V2200/302
Inventor 黄进富吴锦灏
Owner ENPING JIAXIN DAILY NECESSITIES CO LTD
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