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Technology for producing and extracting tryptophan

A tryptophan and process technology, applied in the biological field, can solve the problems of high extraction process cost, difficult recovery, and destruction of active ingredients, and achieve the effects of smooth energy circulation and transmission, improved fermentation acid production performance, and maintenance of growth vitality

Active Publication Date: 2019-12-20
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The traditional extraction process of tryptophan is complicated, and its process is: fermentation broth, plate and frame filtration, vacuum concentration, coarse crystallization, centrifugation, crude product, activated carbon decolorization, recrystallization, centrifugation, drying, packaging and crushing
In this process, Diatomaceous earth filler is required for plate and frame filtration, the decolorization rate is 80%, and the recovery value of filter residue is low The mother liquor of secondary crystallization is turbid and difficult to recover Vacuum concentration is easy to destroy the active ingredients and increase the color of the product due to excessive local temperature, resulting in high energy consumption
[0006] Due to the above aspects, the overall yield of the tryptophan extraction process is below 75%, the cost of the extraction process is high, and the process needs to be improved urgently

Method used

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  • Technology for producing and extracting tryptophan
  • Technology for producing and extracting tryptophan
  • Technology for producing and extracting tryptophan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A process for producing and extracting tryptophan, comprising the steps of:

[0041] 1. Fermentation process:

[0042] Step 1): L-tryptophan engineering bacterium escherichia coli ( E. coli ) TRTH, cultured to a certain concentration of bacterial liquid;

[0043] Step 2): Inoculate the bacterial solution obtained in step 1) with an inoculum amount of 0.5% in a shake flask equipped with a primary seed medium, and culture it at 100-200 rpm for 10-20 hours at 36° C.;

[0044] Step 3): Inoculate the first-level shake flask seeds obtained in step 2) in the seed tank of the second-level seed medium with an inoculation amount of 5%, control the air volume at 5-10L / min, the rotating speed at 200-400rpm, and the temperature at 35-37°C ℃, the tank pressure is 0.05-0.08MPa, the pH value is controlled at 6.5-6.8 by ammonia water, and cultured for 10-20h;

[0045] Step 4): the secondary fermenter seed (OD) that step 3) obtains 600 The value is 11) inoculated with 7% inoculum in a...

Embodiment 2

[0061] A process for producing and extracting tryptophan, comprising the steps of:

[0062] 1. Fermentation process:

[0063] Step 1): L-tryptophan engineering bacterium escherichia coli ( E. coli ) TRTH, cultured to a certain concentration of bacterial liquid;

[0064] Step 2): inoculate the bacterial liquid obtained in step 1) into a shake flask containing a primary seed medium at an inoculum amount of 0.1%-1%, and culture at 36°C at 100-200rpm for 10-20h;

[0065] Step 3): Inoculate the first-level shake flask seeds obtained in step 2) in the seed tank of the second-level seed medium with an inoculation amount of 1%-10%, control the air volume 5-10L / min, the rotating speed 200-400rpm, and the temperature 35-37°C, tank pressure 0.05-0.08MPa, pH value controlled at 6.5-6.8 by ammonia water, culture for 10-20h;

[0066] Step 4): the secondary fermenter seed (OD) that step 3) obtains 600 The value is 12) inoculated with 6% inoculum in a fermenter with fermentation medium, ...

Embodiment 3

[0082] 1. The effect of the amount of dipotassium hydrogen phosphate added on the production of L-tryptophan in the fermentation broth.

[0083] The fermentation process is the same as in Example 1, without using the step of culture medium. Firstly, the effect of feeding dipotassium hydrogen phosphate solution on fermentation was verified by experiments. like figure 1Described, along with the increase of dipotassium hydrogen phosphate flow rate, in the fermented liquid, the content of L-tryptophan increases accordingly, and when flow velocity is 2ml / h, reaches peak substantially, continues to increase flow velocity, has no effect on tryptophan output. significantly increased, and the influence trend of dipotassium hydrogen phosphate on the cell biomass was similar to that of tryptophan (not shown in the figure), the main reason being that adding dipotassium hydrogen phosphate solution could increase the cell biomass, thereby increasing the tryptophan yield.

[0084] 2. The ...

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Abstract

The invention belongs to the technical field of biology, and discloses a technology for producing and extracting tryptophan. The technology comprises a fermentation working procedure and an extractionworking procedure. The production technology is further improved, so that the yield of the tryptophan is increased, and besides, the fed-batch effects are guaranteed; a traditional tryptophan fermentation liquid extracting technology is improved through a membrane technique, thalli protein in the fermentation liquid is removed through a decolorization membrane, and the fermentation liquid is clarified; and macromolecule protein and pigment are subjected to classified removal through the decolorization membrane, and finally concentration is performed under the condition of normal temperature and zero phase changes through a concentration membrane.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a process for producing and extracting tryptophan. Background technique [0002] The molecular formula for L-tryptophan is C 11 h 12 o 2 N 2 , with a molecular weight of 204.21 and a nitrogen content of 13.72%. L-Tryptophan is a neutral aromatic amino acid containing indole groups. It is silky luster, hexagonal flaky self-colored crystals, odorless and sweet. Solubility in water is 1.14g / L (25°C), soluble in dilute acid or alkali, relatively stable in lye, decomposed in strong acid, slightly soluble in ethanol, insoluble in chloroform and ether. [0003] L-Tryptophan is one of the eight essential amino acids in human and animal life activities, and it plays an important role in the growth, development and metabolism of humans and animals. It is called the second essential amino acid, following methionine and lysine Amino acid is the third largest feed additive after...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/22C12N1/20C07D209/20C12R1/19
CPCC12P13/227C12N1/20C07D209/20
Inventor 张宗华边恩来赵仁楷王飞韩富江
Owner XINJIANG FUFENG BIOTECH
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