Strawberry preservation method utilizing linalool fumigation to inhibit botrytis cinerea
A fresh-keeping method and technology of Botrytis cinerea, which are applied in the fresh-keeping, application and food preservation of fruits and vegetables, etc., can solve the problems of membrane potential damage, antibacterial activity and unclear mechanism, so as to reduce the incidence rate, and have a broad commercial application prospect and market. Value, effect of safe operation
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Embodiment 1
[0041] (1) Select local strawberries with a maturity of 70-80%, soak them in 1% sodium hypochlorite aqueous solution for 5 minutes, and then air dry them naturally for 2 hours;
[0042] (2) Make a 1mm hole with a sterile pillow at the equator of the strawberry fruit, and then inoculate 5μl 1×10 4 mL –1 Botrytis cinerea spore suspension;
[0043] (3) 30 strawberry fruits are horizontally placed in a fresh-keeping box with filter paper attached to the inner wall;
[0044] (4) 1.5mL of 125.7mM linalool solution (dissolved in 0.05% Tween 80) is dispersed and dropped on the filter paper in the fresh-keeping box, and immediately sealed so that the final linalool concentration in the fresh-keeping box is 20.95 μ M ;
[0045] (5) Fumigate the sealed strawberries at room temperature (23°C-25°C) for 24 hours, open the cover, and store them in a 90% humidity environment for 4 days to observe the incidence of gray mold in each group.
[0046] The incidence and spot size of gray mold o...
Embodiment 2
[0048] (1) Select local strawberries with a maturity of 70-80%, soak them in 1% sodium hypochlorite aqueous solution for 5 minutes, and then air dry them naturally for 2 hours;
[0049] (2) Make a 1mm hole with a sterile pillow at the equator of the strawberry fruit, and then inoculate 5μl 1×10 4 mL –1 Botrytis cinerea spore suspension;
[0050] (3) 30 strawberry fruits are horizontally placed in a fresh-keeping box with filter paper attached to the inner wall;
[0051] (4) 1.5mL of 1257mM linalool solution (dissolved in 0.05% Tween 80) was dispersed and dropped onto the filter paper in the fresh-keeping box, and immediately sealed so that the final linalool concentration in the fresh-keeping box was 209.5 μM;
[0052] (5) Fumigate the sealed strawberries at room temperature (23°C-25°C) for 24 hours, open the cover, and store them in a 90% humidity environment for 4 days to observe the incidence of gray mold in each group.
[0053] The incidence and spot size of gray mold o...
Embodiment 3
[0055] (1) Select local strawberries with a maturity of 70-80%, soak them in 1% sodium hypochlorite aqueous solution for 5 minutes, and then air dry them naturally for 2 hours;
[0056] (2) Make a 1mm hole with a sterile pillow at the equator of the strawberry fruit, and then inoculate 5μl 1×10 4 mL –1 Botrytis cinerea spore suspension;
[0057] (3) 30 strawberry fruits are horizontally placed in a fresh-keeping box with filter paper attached to the inner wall;
[0058] (4) 1.5ml of 0.05% Tween 80 was used as a contrast, dispersed and dripped onto the filter paper in the fresh-keeping box, and sealed immediately;
[0059] (5) Place the sealed strawberries at room temperature (23°C-25°C) for fumigation for 24 hours, open the cover, and continue to store them in a 90% humidity environment for 4 days, and observe the incidence of botrytis cinerea in each group;
[0060] The incidence of gray mold of each strawberry fruit and the size of the lesion were measured every day, and ...
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