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Glucose electrochemical microelectrode sensor based on nano porous metal film

A technology of glucose sensor and porous metal, which is applied in electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problems of low sensitivity and narrow linear range of the sensor, and achieve the effect of wide response range, easy adjustment of pore size, and high sensitivity

Pending Publication Date: 2019-12-27
ZHENGREN BEIJING MEDICAL INSTR CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems of low sensitivity and narrow linear range of the microelectrode glucose oxidase sensor due to size limitation and small amount of loaded enzyme in the prior art, the present invention provides a method for preparing a microelectrode based on a three-dimensional porous metal membrane and glucose oxidase sensor. Sensor preparation method

Method used

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  • Glucose electrochemical microelectrode sensor based on nano porous metal film
  • Glucose electrochemical microelectrode sensor based on nano porous metal film
  • Glucose electrochemical microelectrode sensor based on nano porous metal film

Examples

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Embodiment 1

[0063] A method for preparing a porous gold film microelectrode, using polyimide as a substrate, magnetron co-sputtering gold and magnesium oxide, washing with acetic acid aqueous solution to remove MgO, and obtaining a porous gold film surface, the specific operation steps are as follows:

[0064] (1) Take a commercial polyimide substrate whose length, width, and thickness are 8 mm, 2 mm, and 0.5 mm, respectively, and treat it with oxygen plasma for 30 minutes, then clean it with water, ethanol, and isopropanol, and dry it with nitrogen. use;

[0065] (2) Magnetron co-sputtering of gold and MgO, the purity of gold and magnesium oxide targets is 99.999%, and the size is 2 inches. During magnetron sputtering, the pressure is 5 millitorr, the substrate speed is 20 rpm, and the substrate is heated to 200 ℃.

[0066] (3) Magnesium oxide uses 150W, and gold uses different powers to obtain different proportions of co-sputtering films:

[0067]

[0068] (4) The obtained coating ...

Embodiment 2

[0074] The second embodiment of the present invention provides a glucose electrochemical biosensor based on the preparation of the porous gold film microelectrode in the first embodiment, and the specific operation steps are as follows:

[0075] (1) choose sputtering 70% magnesia ratio, the sample that total thickness is 100nm carries out this embodiment, under this condition, pore aperture is in the scope of 20-100nm;

[0076] (2) modify a layer of Prussian blue in the pores of the porous gold film, the method is:

[0077] Dip the microelectrodes into concentrated HNO with a volume ratio of 1:3:4 3 :HCl:H 2 O solution for 3 minutes, thoroughly wash the surface of the electrode with deionized water, wash it repeatedly with ethanol and deionized water, and dry it with nitrogen gas;

[0078]One side of the porous gold film is used as the working electrode, the Ag / AgCl side is used as the reference electrode, and the Pt wire is used as the counter electrode. 3 [Fe(CN) 6 ], 2....

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Abstract

The invention discloses a preparation method of a nano porous metal film and a microelectrode sensor loaded with glucose oxidase (GOD) on the porous film for blood glucose monitoring, and relates to aprocessing technology of the porous metal film and a sensor technology. Metal and metal oxides of different proportions and thicknesses are subjected to magnetron co-sputtering on a polyimide substrate, treatment is conducted through deionized water or a weak acid solution to remove the metal oxides, metal layers which are each of a three-dimensional porous network structure and are interconnected are left to serves as a support layer and a conductive layer which are loaded with the GOD, the other side of the insulated substrate is coated with Ag / AgCl to serve as a reference-counter electrode, thus an electrochemical two-electrode system is constituted, then the substrate is coated with a high-molecular polymer to serve as a diffusion-limiting layer and an anti-interference hydrophilic layer, and then a glucose sensing microelectrode is constituted. The nano porous metal film is large in specific surface area, the active area of the electrode and the load amount of the GOD are effectively increased, permeability is good, conductivity is high, and physical damage to a microelectrode enzyme film sensing layer can be further prevented to a certain extent. The microelectrode sensor iswide in linear range and good in long-term stability.

Description

technical field [0001] The invention belongs to the field of electrochemical biosensors, and relates to a processing technology of a metal film with a porous structure, a microelectrode and an electrochemical glucose sensor based on the porous metal film, and their preparation methods. Background technique [0002] Diabetes is one of the common chronic diseases that seriously threaten human health. Real-time monitoring of blood glucose is helpful for patients and doctors to evaluate the condition, formulate reasonable and scientific treatment plans or adjust existing plans. Therefore, glucose sensors are also the most researched and commercial products in the field of biosensors. the earliest biosensors. The core component of the glucose sensor is the enzyme electrode composed of glucose oxidase (GOD) effectively immobilized on the bioactive interface. In practical applications or existing marketed blood glucose monitoring sensors generally use needle-type microelectrodes, ...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3272G01N27/48
Inventor 不公告发明人
Owner ZHENGREN BEIJING MEDICAL INSTR CO LTD
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