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A kind of bio-enzyme composite preparation for oil recovery and its preparation method and application

A compound preparation and biological enzyme technology, applied in chemical instruments and methods, production fluids, earth-moving drilling, etc., can solve the problems of low activation efficiency of anaerobic microorganisms, unstable oil well huff and puff effect, poor adaptability of bacterial strains to oil reservoirs, etc. Achieve the effect of good on-site test effect, wide application range and lower process cost

Active Publication Date: 2022-01-28
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this invention are: the exogenous fermentation bacteria are affected by the reservoir temperature, oxygen, salinity and pressure, resulting in poor reservoir adaptability of the bacteria and unstable oil well huff and puff effect; The range of interaction with oil reservoirs is limited, which is not conducive to the full contact of microorganisms with crude oil to play a role
The disadvantage of this method is that it is suitable for oil wells with surface crude oil viscosity less than 3000mPa.s, and not suitable for oil wells with higher viscosity; the injection of air only activates some aerobic microorganisms in the near-wellbore area, and the range of action is small, while the area rich in remaining oil is often in the In the anaerobic area deep in the reservoir, the activation efficiency of anaerobic microorganisms is low

Method used

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  • A kind of bio-enzyme composite preparation for oil recovery and its preparation method and application
  • A kind of bio-enzyme composite preparation for oil recovery and its preparation method and application
  • A kind of bio-enzyme composite preparation for oil recovery and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0042] The preparation of the biological enzyme composite preparation A of embodiment 1 oil recovery

[0043] (1) Dissolving the functional microbial fermentation liquid in water, using a magnetic stirrer to stir at room temperature for 20 minutes at a speed of 175 rpm, to obtain a functional microbial fermentation liquid solution;

[0044] (2) Add nutrients to the functional microbial fermentation broth solution, and stir for 40 minutes at a rotation speed of 300 rpm and a temperature of 30° C. to obtain a mixed solution;

[0045] (3) The biological enzyme was added to the above mixed solution at a constant speed under the condition of heating while stirring. The stirring speed was 400 rpm, the stirring time was 75 min, and the heating temperature was 40 ° C. After the stirring was completed, the compound preparation A was obtained.

[0046] The composition and components (mass percentage) of the biological enzyme compound preparation A for oil recovery are as follows:

[0047...

Embodiment 2

[0052] The preparation of the biological enzyme compound preparation B of embodiment 2 oil recovery

[0053] (1) Dissolving the functional microbial fermentation liquid in water, using a magnetic stirrer to stir at room temperature for 30 minutes at a speed of 150 rpm, to obtain a functional microbial fermentation liquid solution;

[0054] (2) Add nutrients to the functional microbial fermentation broth solution, and stir for 30 minutes at a rotation speed of 250 rpm and a temperature of 35° C. to obtain a mixed solution;

[0055] (3) The biological enzyme was added to the above mixed solution at a constant speed under the condition of heating while stirring. The stirring speed was 350 rpm, the stirring time was 60 min, and the heating temperature was 35 ° C. After the stirring was completed, the compound preparation B was obtained.

[0056] The composition and components (mass percentage) of the biological enzyme compound preparation B for oil recovery are as follows:

[005...

Embodiment 3

[0061] The preparation of the biological enzyme compound preparation C of embodiment 3 oil recovery

[0062] (1) Dissolving the functional microbial fermentation liquid in water, using a magnetic stirrer to stir at room temperature for 25 minutes at a speed of 200 rpm, to obtain a functional microbial fermentation liquid solution;

[0063] (2) Add nutrients to the functional microbial fermentation broth solution, and stir for 50 minutes at a rotation speed of 200 rpm and a temperature of 32° C. to obtain a mixed solution;

[0064] (3) The biological enzyme was added to the above mixed solution at a constant speed under the condition of heating while stirring. The stirring speed was 375rpm, the stirring time was 90min, and the heating temperature was 37.5°C. Composite preparation C was obtained after the stirring was completed.

[0065] The composition and components (mass percentage) of the biological enzyme compound preparation C for oil recovery are as follows:

[0066] ...

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Abstract

The invention belongs to the technical field of petroleum exploitation, and in particular relates to a biological enzyme compound preparation for oil exploitation, its preparation method and application. The composition and components (mass percentage) of the compound preparation are as follows: 1-2% of functional microbial fermentation liquid; 5-10% of nutrient; 0.5-1.0% of biological enzyme; the rest is water. The functional microorganism fermentation liquid is the fermentation liquid of thermotoga or thermodenitrophilic bacillus. The nutrient is composed of a nitrogen source and a phosphorus source, the nitrogen source is one of ammonium chloride, urea and sodium nitrate, and the phosphorus source is one of dipotassium hydrogen phosphate, disodium hydrogen phosphate and diammonium hydrogen phosphate. Biological enzymes consist of acetyl xylan esterase and alkyl succinate synthase. The invention has the characteristics of simple construction technology, strong operability and good field test effect, the effective period of the oil well is more than 50 months, the average daily oil increase of a single well is more than 10 tons, and the input-output ratio is more than 1:10.

Description

technical field [0001] The invention belongs to the technical field of petroleum exploitation, and in particular relates to a biological enzyme compound preparation for oil exploitation, its preparation method and application. Background technique [0002] The oil issue has become the main energy issue in today's world, and the whole world is facing the problem of increasing shortage of oil resources. With the rapid development of my country's economy, the demand for crude oil continues to expand, and the contradiction between production capacity and demand is increasingly intensified. At present, the conventional oil recovery technology can only recover 30% to 40% of the crude oil in the underground oil reservoir, and the total amount of crude oil remaining in the formation far exceeds the new reserves that can be discovered. Therefore, how to extract more crude oil from underground and effectively enhance oil recovery has become an urgent problem. Microbial enhanced oil ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K8/582E21B43/22
CPCC09K8/582E21B43/16
Inventor 汪卫东冯云林军章吴晓玲郭辽原谭晓明陈琼瑶王新耿雪丽张吉星
Owner CHINA PETROLEUM & CHEM CORP
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