Granular cell stripping solution and preparation method thereof

Abstract

The invention discloses a granular cell stripping solution and a preparation method thereof. The granular cell stripping solutionis prepared fromabase solutioncontaining an antibacterial agent, a phenol red indicator, 20 to 22mmol / Lof hyaluronidase, andaprotein additive, namely human serum albumin, 33 to 36mmol / L ofHEPES, 10.5 to 12.0 mmol / L ofTES, 10.5 to 12.0 mmol / L of DIPSO , and 3.5 to 5.0 mmol / L of arginine. The compositions can provide effective functions for stripping of cumulus cells of oocytes and surrounding granular cell clusters, provide sufficient energy substances during in vitrostripping operation, and provide a more stable buffer system, and increase the fertilization rate, special nutrients needed during the periodoflater stage of fertilizationare also provided forthe oocytes at the later stage of fertilization, an ideal environment is provided for in vitro treatmentand pre-cultivation of ova, and further development of a reproduction technology and increasing of thesuccess rate are assisted advantageously.

Description

technical field [0001] The invention belongs to the field of human assisted reproduction, and in particular relates to a granule cell stripping liquid applied to human assisted reproductive medicine technology and a preparation method. Background technique [0002] Granulosa cell stripping solution is a widely used product in assisted reproductive technology. The granulosa cell stripping solution is to separate the cumulus cells and cumulus complexes around the egg, and hyaluronidase is added to the basic culture solution components. Hyaluronidase An enzyme that attacks the hexosamine bonds of hyaluronic acid, a glycosaminoglycan that makes up the extracellular matrix that holds cumulus cells together, in the ICSI (Intracytoplasmic Sperm Injection) technique Before the application, it is necessary to ensure the quality of oocytes and avoid loss. [0003] Now, in China's assisted reproductive technology market, the use of granulosa cell stripping solution relies on imported...

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Problems solved by technology

[0003] Now, in China's assisted reproductive technology market, the use of granule cell stripping solution depends on imported finished products, which not only costs high, but more importantly, the effectiveness of the solution cannot be well guaranteed

Continuing to use the granulosa cell stripping solution after losing its potency will affect the quality of oocyte processing and cause adverse consequences on the quality of oocyte processing, the formation and development of fertilized eggs

[0004] At present, domestic assisted reproductive fluids rely on imports, but there is no domestic independent production of such products, and there is no room for bargaining. The high price leads to high medical costs for patients and increases the economic burden.

Imported products often need to be transported over long distances. During the long-distance transportation, the storage conditions cannot be guaranteed, and the transportation time is long, which makes the use of the culture medium less effective. It cannot provide the best effect on the clinical egg and embryo culture, which directly affects the quality of the embryo. development, adverse consequences

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