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Preparation and application of an ultra-bright fluorescent carbon dot

A fluorescent carbon dot and ultra-bright technology, applied in the field of nanomaterials and biology, can solve the problems of low fluorescence quantum yield, high synthesis cost, and short imaging time, and achieve the effect of simple operation method, low cost, and simple operation

Active Publication Date: 2022-06-24
SHANDONG NORMAL UNIV
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Problems solved by technology

[0005] In order to overcome the above problems, the present invention provides an ultra-bright fluorescent carbon dot (P-R CDs) to achieve long-term stable targeted imaging of lysosomes, which solves the problems of low fluorescence quantum yield and synthesis cost in the prior art. High, complex procedures, short imaging time, etc.

Method used

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  • Preparation and application of an ultra-bright fluorescent carbon dot
  • Preparation and application of an ultra-bright fluorescent carbon dot
  • Preparation and application of an ultra-bright fluorescent carbon dot

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preparation example Construction

[0047] The preparation method of the above-mentioned ultra-bright fluorescent carbon dots comprises the following steps:

[0048] Dissolve the formula amount of RB in ultrapure water, add the formula amount of bPEI to react; after the reaction is completed, the reaction solution cooled to room temperature is dialyzed to obtain high-purity fluorescent carbon dots.

[0049] in,

[0050] The reaction temperature is 25-200°C, preferably 160°C;

[0051] The reaction time is 2~24h, preferably 6h;

[0052] Raw material concentration ratio (bPEI:RB) 0.5:1~5:1, preferably 3:1

[0053] The reaction device is preferably a hydrothermal reactor.

[0054] Wherein the molecular weight cut-off of the dialysis bag used for dialysis is 500-1000.

[0055] The application of the above-mentioned ultra-bright fluorescent carbon dots as lysosome-targeted fluorescent probes also falls within the protection scope of the present invention.

Embodiment 1

[0058] Synthesis of P-R CDs:

[0059] 1 mL of bPEI diluent was mixed with 4 mL of 15 mg / mL RB solution, and the mixture was transferred to a 10 mL autoclave, hydrothermally reacted at 160 °C for 6 h, cooled to room temperature, and the crude P-RCDs solution was obtained by intercepting Dialysis with a dialysis belt with a molecular weight of 500-1000 for 12 hours.

Embodiment 2

[0061] Colocalization analysis of P-R CDs for lysosome-targeted imaging:

[0062] In an incubator, HL-7702 cells were incubated with 150 μL, 1 mg / mL of P-R CDs for 10, 20, 30, and 60 minutes, respectively. When the incubation was completed, the old medium was discarded, washed three times with PBS, and fresh medium was added for imaging. . In particular, before imaging, commercial Lyso Tracker Red (Lyso Red) dye needs to be added half an hour in advance to visualize lysosomes, and SP8 confocal microscope is used for fluorescence imaging analysis.

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Abstract

The invention relates to the preparation and application of a super-bright fluorescent carbon dot, which is made of the following raw materials in parts by weight: 10-12 parts of polyethyleneimine, 1-10 parts of Bengal rose red, and realizes long-term stable lysosomes Targeted imaging solves the problems of low fluorescence quantum yield, high synthesis cost, complicated procedure, and short imaging time in the prior art.

Description

technical field [0001] The invention belongs to the field of nanomaterials and biotechnology, and particularly relates to the preparation and application of an ultra-bright fluorescent carbon dot. Background technique [0002] The disclosure of information in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] There are many kinds of fluorescent materials and are widely used. Traditional fluorescent organic small molecules are complex in synthesis and unstable in fluorescence; traditional semiconductor quantum dots are also limited in application due to their potential toxicity; fluorescent carbon materials, as an emerging fluorescent material, are widely used in biology due to their excellent properties. , medicine, electronics an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00A61K49/00
CPCC09K11/65B82Y20/00B82Y40/00A61K49/0019
Inventor 唐波佟丽丽王秀秀
Owner SHANDONG NORMAL UNIV
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