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Leersia hexandra Swartz endophytic bacterium capable of reducing hexavalent chromium and preparation method and application thereof

A technology of endogenous bacteria and hexavalent chromium, applied in biochemical equipment and methods, microorganism-based methods, bacteria, etc.

Active Publication Date: 2020-01-10
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the use of endophytic bacteria of Lishihe graminearum to the heavy metal Cr( ) reduction research has not been reported yet

Method used

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  • Leersia hexandra Swartz endophytic bacterium capable of reducing hexavalent chromium and preparation method and application thereof
  • Leersia hexandra Swartz endophytic bacterium capable of reducing hexavalent chromium and preparation method and application thereof
  • Leersia hexandra Swartz endophytic bacterium capable of reducing hexavalent chromium and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Isolation and Screening of Chromium Reducing Endophytic Bacteria

[0022] First, the Lishihe plant was collected from the Phytoremediation Laboratory of the School of Environmental Science and Engineering, Guilin University of Technology, and an appropriate amount of healthy Lishihe stem was washed with water, soaked in 70% alcohol for 30 s under sterile conditions, and then soaked with 2.5 % sodium hypochlorite for 1 min, and finally rinsed with sterile water 6 times to remove the disinfectant attached to the surface of the material. Under aseptic conditions, use the sterile water rinsed for the last time to spread on the beef extract peptone solid plate medium. After cultivation, no microorganisms grow out, indicating that the surface is thoroughly disinfected. Under sterile conditions, take an appropriate amount of surface-sterilized root tissue, add 1 mL of 0.9% sodium chloride solution to grind thoroughly, take 1 mL of the grinding liquid and inoculate it in 100 mL...

Embodiment 2

[0026] Example 2 The initial pH of the J01 strain reduced Cr ( )Impact

[0027] The strain J01 stored on the slant of the test tube was activated by beef extract-peptone solid medium plate, cultured in a constant temperature incubator at 37 °C for 24 hours, and then 2 rings were picked and inoculated into a 250 mL Erlenmeyer flask containing 100 mL beef extract-peptone liquid medium. After 24 hours of shaking culture at 37 °C and 120 r / min, it was used as the seed solution.

[0028] The seed liquid was inoculated into the beef extract peptone liquid medium (filling volume 100 mL / 250 mL Erlenmeyer flask) containing Cr(VI) concentration of 120 mg / L according to 10% inoculum amount, with 2 M sodium hydroxide and After adjusting the pH with hydrochloric acid solution, eight layers of gauze were sealed, and placed on a constant temperature horizontal shaker at 35 °C for shaking culture at 150 r / min. After culturing for 48 h, an appropriate amount of culture fluid was sampled und...

Embodiment 3

[0029] Example 3 Temperature reduces Cr to J01 strain ( )Impact

[0030] The strain J01 stored on the slant of the test tube was activated by beef extract-peptone solid medium plate, cultured in a constant temperature incubator at 37 °C for 24 hours, and then 2 rings were picked and inoculated into a 250 mL Erlenmeyer flask containing 100 mL beef extract-peptone liquid medium. After 24 hours of shaking culture at 37 °C and 120 r / min, it was used as the seed solution.

[0031]The seed liquid was inoculated into the beef extract peptone liquid medium (100 ml) containing Cr(VI) concentration of 120 mg / L and pH 6.0 (adjusted with 2 M sodium hydroxide and hydrochloric acid solution) according to 10% inoculum amount. mL / 250 mL Erlenmeyer flask), sealed with eight layers of gauze, and placed on a constant temperature horizontal shaker at different temperatures for shaking culture at 150 r / min. After culturing for 48 h, an appropriate amount of culture fluid was sampled under steri...

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Abstract

The invention discloses aLeersia hexandra Swartz endophytic bacteriumcapable of reducing hexavalent chromium. The Leersia hexandra Swartzendophytic bacterium is Bacillus cereus J01 which is isolated by an inventor from stem tissue of chromium superaccumulative plant Leersia hexandra Swartz in a laboratory of the School of Environmental Science and Engineering of Guilin University of Technology, and is preserved in the General Microbial Center of the Chinese Commission for the Preservation and Management of Microbial Specieson June 22, 2017, and the preservation number is CGMCC NO.14267. The Leersia hexandra Swartz endophytic bacterium can reduce hexavalent chromium to trivalent chromium, and a new microbial resource is provided for microbial remediation of hexavalent chromium contamination.

Description

technical field [0001] The invention relates to the field of bacterial strains, in particular to an endophytic bacterium capable of reducing hexavalent chromium and its preparation method and application. Background technique [0002] With the development of modern technology, heavy metal chromium has been widely used in many fields such as pharmaceuticals and electroplating. But at the same time, the pollution problem of chromium and its compounds in the environment is also becoming more and more serious. Long-term exposure to chromium and chromium compounds can easily affect the gastrointestinal system, immune system, liver and kidneys and even induce respiratory system cancer. Among the various forms of chromium, Cr mainly exists in the form of chromate and dichromate ( ) are considered to be the most toxic. Internationally, the bioremediation method is advocated for chromium-contaminated water and soil, that is, the removal of heavy metal ions by means of flocculatio...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/085C02F101/22
CPCC12N1/20B09C1/10C02F3/34C02F2101/22C12R2001/085C12N1/205
Inventor 李海云张蕴勃李子院董新红李霞李培骏单杨
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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