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Method for preparing cannabidiol

A cannabidiol and enzymatic hydrolysis technology, applied in the field of cannabidiol preparation, can solve the problems of low extraction purity, complicated operation process, poor product quality and the like, and achieve the effects of stable product quality, good separation effect, and increased content

Active Publication Date: 2020-01-14
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in existing processes, such as macroporous resin extraction method, silica gel column chromatography adsorption method, etc., or there is a problem of low extraction purity; or the operation process is cumbersome and the process conditions are not easy to control; or the recovery of cannabidiol The rate is low and the product quality is not good
In addition, in the existing process, a large amount of organic solvents are used in general solvent treatment, which is not conducive to environmental protection and large-scale industrial production.

Method used

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  • Method for preparing cannabidiol

Examples

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preparation example Construction

[0027] figure 1 A process flow chart for preparing cannabidiol according to one embodiment of the present invention is shown. Such as figure 1 As shown, the method for preparing cannabidiol in the present invention mainly includes enzymatic hydrolysis reaction, flash extraction, alumina column chromatography, organic nanofiltration membrane treatment and crystallization treatment. specifically:

[0028] (1) Enzymolysis reaction

[0029] First, a plant source containing cannabidiol is provided as a raw material and pulverized. Wherein the plant source may be a plant of the genus Cannabis (Cannabis) of the family Cannabinaceae containing cannabidiol.

[0030] The pulverized raw materials, water and biological enzymes are mixed evenly and then the enzymolysis reaction is carried out. In some embodiments, the mesh of the pulverized raw material is 40-100 mesh, so that the raw material can be better mixed with water and biological enzymes. In some embodiments, the mass ratio ...

Embodiment 1

[0055] (1) Enzymolysis reaction

[0056] Take 1000g of cannabis plant containing cannabidiol and grind it into 40 meshes with a pulverizer, then add 4 times the amount of water with a high-pressure homogenizer and smash it 3 times, each time for 10 minutes. Pour the mixed solution homogenized under high pressure into the reaction kettle, and add biological enzymes. The amount of biological enzyme added is 1g, and the biological enzyme comprises papain, amylase and cellulase, and the mass ratio is 1:1:1. After carrying out the enzymolysis reaction at 45° C. for 1.5 hours, the solution was passed through a 100-mesh filter cloth, and the enzymolysis solution was discarded to obtain an enzymolysis residue.

[0057] (2) Flash extraction

[0058] Add the enzymolysis residue obtained in step (1) into a flash extractor, and use 75% ethanol as the extraction solvent, wherein the mass ratio of the enzymolysis residue to the extraction solvent is 1:18. The extraction speed of the flas...

Embodiment 2

[0066] Adopt the same method as Example 1 to prepare cannabidiol, the difference is that the temperature of the enzymolysis reaction in step (1) is 50°C, the reaction time is 1.9h, and the CBD content in the obtained cannabidiol extract is 99.3% .

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Abstract

The invention provides a method for preparing cannabidiol. The method comprises the following steps: providing a plant source with cannabidiol as a raw material, crushing the raw material, mixing thecrushed raw material, water and a biological enzyme, and performing an enzymolysis reaction, performing filtering so as to obtain an enzymolysis liquid and enzymolysis residues; mixing the enzymolysisresidues with an extraction solvent, and performing flash extraction at room temperature, wherein the mass ratio of the enzymolysis residues to the extraction solvent is (1:18)-(1:20); performing solid-liquid separation on the extraction material liquid obtained after flash extraction, and collecting a liquid phase so as to obtain a cannabidiol extract; performing alumina column chromatography separation on the cannabidiol extract so as to obtain an eluant; treating the eluant by using an organic nanofiltration membrane so as to obtain a membrane through liquid; and performing crystallizationtreatment on the membrane through liquid, so as to obtain the cannabidiol. By adopting the method, a conventional preparation method is replaced by a method with the combination of a biological enzymolysis method and flash extraction, the extraction rate of the cannabidiol can be effectively increased, meanwhile, by adopting a separation mode with the combination of alumina column chromatographyand the organic nanofiltration membrane, a high-purity CBD (cannabidiol) product is further prepared, and the purity of the CBD can be all up to 99% or greater.

Description

technical field [0001] The invention relates to the technical field of chemical industry, in particular to a preparation method of cannabidiol. Background technique [0002] Industrial hemp refers to the original plant of the genus Cannabis and its extracted products with a tetrahydrocannabinol content of less than 0.3%. It is widely used in many fields such as biopharmaceuticals, food additives, daily necessities, textile fibers, and composite materials. The main cannabinoids in cannabis plants are tetrahydrocannabinol (THC), cannabinol (CBN), cannabidiol (CBD), cannabigerol (CBG), and cannabichromene (CBC), among which the first three Those account for 90% of cannabinoids. [0003] Tetrahydrocannabinol (THC) in marijuana is the main psychoactive chemical component in marijuana, which can cause euphoria and addiction, and is the main reason why marijuana is recognized as a drug. Cannabidiol (CBD) is a non-addictive component in marijuana and has high medicinal value, so i...

Claims

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Application Information

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IPC IPC(8): C07C37/68C07C37/70C07C37/82C07C37/84C07C37/86C07C39/23
CPCC07C37/004C07C2601/16C07C39/23
Inventor 赵军秦本军宋云飞
Owner GUILIN NATURAL INGREDIENTS CORP