A kind of wheat gluten active peptide and its preparation method and application
A technology of wheat gluten protein and wheat gluten, which is applied in the biological field to achieve the effect of mild conditions, high purity and strong activity
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Embodiment 1
[0042] A preparation method of wheat gluten peptide capable of improving the physiological activity and fermentation performance of Saccharomyces cerevisiae in ultra-high concentration ethanol fermentation, the specific steps are as follows:
[0043] (1) Preparation of wheat gluten hydrolyzate: mix wheat gluten and water with water at a ratio of 1:8 (m / m), add trypsin (based on the amount of wheat gluten protein, the amount of trypsin added is 1 wt%), stirred at 45°C for 20 h, then inactivated the enzyme in boiling water for 10 min, cooled to room temperature, centrifuged at 4000 r / min for 5 min, removed the precipitate, and obtained the enzymatic hydrolysis liquid of wheat gluten protein, and obtained the enzymatic hydrolysis of wheat gluten protein freeze-dried powder;
[0044] (2) Sephadex G-15 gel column separation of enzymatic hydrolyzate of wheat gluten: elute the enzymatic hydrolyzate of wheat gluten with deionized water at a flow rate of 0.5 mL / min, the detection wavel...
Embodiment 2
[0048] A preparation method of wheat gluten peptide capable of improving the physiological activity and fermentation performance of Saccharomyces cerevisiae in ultra-high concentration ethanol fermentation, the specific steps are as follows:
[0049] (1) Preparation of wheat gluten hydrolyzate: mix wheat gluten and water with water at a ratio of 1:9 (m / m), add trypsin (based on the amount of wheat gluten protein, the amount of trypsin added is 1.5 wt%), stirred at 50°C for 22 h, then inactivated the enzyme in boiling water for 20 min, cooled to room temperature, centrifuged at 8000 r / min for 10 min, and removed the precipitate to obtain the enzymatic hydrolysis liquid of wheat gluten protein, and obtained the enzymatic hydrolysis of wheat gluten protein freeze-dried powder;
[0050] (2) Sephadex G-15 gel column separation of enzymatic hydrolyzate of wheat gluten: elute the enzymatic hydrolyzate of wheat gluten with deionized water at a flow rate of 1 mL / min, the detection wave...
Embodiment 3
[0054] A preparation method of wheat gluten peptide capable of improving the physiological activity and fermentation performance of Saccharomyces cerevisiae in ultra-high concentration ethanol fermentation, the specific steps are as follows:
[0055] (1) Preparation of enzymatic hydrolyzate of wheat gluten protein: mix wheat gluten protein and water with water at a ratio of 1:10 (m / m), add trypsin (based on the amount of wheat gluten protein, the amount of trypsin added is 1 wt%), stirred at 55°C for 24 h, then inactivated the enzyme in boiling water for 30 min, cooled to room temperature, centrifuged at 12,000 r / min for 15 min, and removed the precipitate to obtain the enzymatic hydrolysis liquid of wheat gluten protein, and obtained the enzymatic hydrolysis of wheat gluten protein freeze-dried powder;
[0056] (2) Sephadex G-15 gel column separation of enzymatic hydrolyzate of wheat gluten: elute the enzymatic hydrolyzate of wheat gluten with deionized water at a flow rate o...
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