Unlock instant, AI-driven research and patent intelligence for your innovation.

Amino acid nano hydrogel and its preparation method and application

A nano-hydrogel, amino acid technology, applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of uneven imprinted pores, many action sites, and collapse of recognition sites, and achieve a good biological phase. Capacitance, mild reaction conditions, improved recognition ability

Active Publication Date: 2021-05-11
SOUTHERN MEDICAL UNIVERSITY
View PDF15 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned polymers with imprinted holes prepared by molecular imprinting technology must use template proteins, cloned proteins or specific peptides as template molecules in the preparation process, and these expensive template protein molecules with unstable physical and chemical properties During the imprinting process, the structure will be denatured due to the elution of acidic and polar solvents, making it impossible to reuse; and the template elution often has incomplete elution, which will lead to uneven imprinted holes and affect the polymer. Absorptivity, even leakage occurs when the polymer adsorbs the target protein, resulting in inaccurate adsorption and other problems
In addition, the protein itself has a complex structure and many action sites, and the three-dimensional network structure of the prepared polymer is unstable, which may easily cause the collapse of the recognition site.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Amino acid nano hydrogel and its preparation method and application
  • Amino acid nano hydrogel and its preparation method and application
  • Amino acid nano hydrogel and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Preparation of polymer A, that is, hydrophobic amino acid nanohydrogels, including phenylalanine nanohydrogels and leucine nanohydrogels.

[0077] 1. Preparation of functional monomer N-acryloyl-L-phenylalanine

[0078] Dissolve 3.63g (0.020mol) of L-phenylalanine (L-Phe) and 1.6g (0.04mol) of sodium hydroxide in 20mL of water, and slowly add 1.75mL (0.022mol) of propylene dropwise while stirring at below 0°C Acyl chloride, drop it within 0.5h. After the dropwise addition was completed, stirring was continued at room temperature for 6 h. Adjust the pH to 2 with concentrated hydrochloric acid, filter with suction, wash with water until neutral, and dry under vacuum at 50°C for 24 hours to obtain a white powdery solid, N-acryloyl-L-phenylalanine (APA), with a yield of 45.7% (2.74 g).

[0079] 2. Preparation of functional monomer N-acryloyl-L-leucine

[0080] Dissolve 2.6238g (0.02mol) of L-leucine and 1.6g (0.04mol) of sodium hydroxide in 30ml of water, and slowly add...

Embodiment 1-2

[0088] Preparation of polymer A, that is, hydrophobic amino acid nanohydrogels, including phenylalanine nanohydrogels and leucine nanohydrogels. The steps of preparing phenylalanine nano-hydrogel in this embodiment are as follows:

[0089] N-acryloyl-L-phenylalanine (APA) (285mg, 40mol%), N-isopropylacrylamide (213.3mg, 58mol%), N,N'-methylenebisacrylamide (10mg, 2mol%), ammonium sulfate (30mg) was dissolved in 50ml water containing 10mg sodium lauryl sulfate.

[0090] Nitrogen was passed for 20 minutes, and the reaction was heated in an oil bath at 65°C for 3 hours. The obtained polymer solution was put into a dialysis bag (MW 12000-14000) for dialysis for 4 days, and the water was changed twice a day. The dialyzate inside the dialysis membrane was collected and freeze-dried to obtain phenylalanine nanohydrogels (APA@NPs).

[0091]In the same way, leucine nanohydrogels (A-Leu@NPs) were prepared, but the functional monomer N-acryloyl-L-leucine (A-Leu, 240.7mg, 40mol%) repla...

Embodiment 2

[0093] Preparation of polymer B, positively charged amino acid nanohydrogels, including arginine nanohydrogels and histidine nanohydrogels.

[0094] 1. Preparation of functional monomer N-acryloyl-arginine

[0095] Dissolve 1.7420g (0.01mol) of L-arginine and 2.12g (0.02mol) of sodium carbonate in 30ml of water, place it below 0°C, slowly add 0.95ml (0.012mol) of acryloyl chloride dropwise while stirring, and drop it within 0.5h Finish. After the dropwise addition was completed, stirring was continued at room temperature for 3.0 h. Adjust the pH to 2 with concentrated hydrochloric acid, wash with saturated sodium chloride solution, extract with ethyl acetate:isopropanol (1:1) 2×40ml, dry the organic layer over anhydrous magnesium sulfate, filter, and evaporate under reduced pressure solvent. The residue was separated by silica gel column chromatography [V(chloroform):V(methanol)=1:1] to obtain N-acryloyl-arginine (A-Arg) as a yellow liquid with a yield of 15.00%.

[0096] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
number average molecular weightaaaaaaaaaa
particle sizeaaaaaaaaaa
particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses an amino acid nano hydrogel and its preparation method and application. The amino acid nano hydrogel has amino acid side chains, and can interact with proteins similar to natural proteins-natural proteins, and is harmful to humans. Serum proteins and biomarkers such as β-amyloid, brain natriuretic peptide, and gastrin-releasing peptide precursor have specific recognition capabilities and can be used to adsorb target proteins. The invention improves the recognition ability of the polymer, has simple preparation method, mild reaction conditions, has temperature response characteristics, and has the potential of being developed into a stable performance artificial analog antibody or protein purification polymer material. The amino acid nano hydrogel is polymer A or polymer B; the polymer A is composed of fragments shown in formula (I), formula (II) and formula (III); the polymer B is composed of Fragments represented by formula (IV), formula (II), formula (V) and formula (III);

Description

technical field [0001] The present invention relates to the technical field of non-biological antibodies, more specifically, to an amino acid nano hydrogel and its preparation method and application. Background technique [0002] Antibodies currently play an important role in clinical testing, diagnosis and treatment of diseases, and purification of antigens and proteins. The artificial preparation of natural antibodies is mainly obtained through methods such as polyclonal antibodies, monoclonal antibodies, and genetically engineered antibodies. Among them, monoclonal antibodies are the most commonly used in clinical diagnostic testing, but the preparation cycle is long, the cost is high, and it is difficult to produce on a large scale, so the price is expensive , and cannot be recycled; on the other hand, antibodies, like proteins, are sensitive to the environment, have poor stability, and are easily inactivated under high temperature and harsh conditions, which brings grea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/54C08F220/58C08F222/38C08F220/60C08J3/075C07K14/765C07K1/14C08L33/24
CPCC07K14/765C08F220/54C08F220/60C08J3/075C08J2333/24C08F220/58C08F222/385
Inventor 朱全红朱泳妍吴登宇
Owner SOUTHERN MEDICAL UNIVERSITY