Method for applying L-selenomethionine to animal cell oxidation resistance and product thereof

A technology of selenomethionine and animal cells, which is applied in the field of L-selenomethionine in the anti-oxidation of animal cells, can solve the problem of reduced glutathione peroxidase activity, reduced glutathione content, and increased ROS levels. High-level problems, to achieve the effect of improving the ability to resist oxidative damage, requiring extensive additions, and mitigating parameter changes

Pending Publication Date: 2020-01-24
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

[0002] When the animal body or animal cells are stimulated by harmful stimuli in vivo or in vitro, such as some oxidative substances, they can produce oxidative stress. This oxidative stress will cause changes in a series of substances in cells, such as: intracellular valley The content of glutathione (GSH) decreases, the activity of glutathione peroxidase (GPx) decreases, and the level of intracellular ROS increases, which in turn induces the production of intracellular malondialdehyde (MDA), and the level of intracellular MDA increases , induc

Method used

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  • Method for applying L-selenomethionine to animal cell oxidation resistance and product thereof
  • Method for applying L-selenomethionine to animal cell oxidation resistance and product thereof
  • Method for applying L-selenomethionine to animal cell oxidation resistance and product thereof

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preparation example Construction

[0076] The primary broiler hepatocytes in the embodiment are prepared by the following method:

[0077]Purchase commercially available 35-day-old healthy white-feathered AA broiler chickens, inject 3 mL of 200 U heparin sodium into the subwing vein after anesthesia, wash the whole body with fresh cleansing solution, and keep the broilers on the operating table. Open the abdominal cavity to expose the liver; ligate the perihepatic blood vessels, cut off all the ligated blood vessels, separate the connective tissue, remove the whole liver, wash the blood clot on the liver surface with 37°C D-Hank's buffer (no glucose), and Place in 37°C penicillin-streptomycin normal saline, and then perform perfusion. Find the hepatic portal vein on the ventral side of the chicken liver, cut a small opening, insert the catheter that removes the scalp needle into the vein, and ligate it. The hot perfusion solution A is slowly poured into the liver. When the liver expands slowly, use a needle to...

Embodiment 1

[0079] After the broiler hepatocyte suspension was cultured for 24 hours, the cells adhered to the wall, and the obtained broiler primary hepatocyte culture system was used as the animal cell culture system. (Note: 6-well plates and 96-well plates were plated with broiler liver cell suspension; the 6-well plates were subsequently used for HE staining to observe cell morphology, determination of malondialdehyde content, determination of glutathione content, glutathione peroxidation Determination of enzyme content; 96-well plate was used for cell activity determination; both were cultured for 24 hours as the primary broiler hepatocyte culture system in subsequent experiments.)

[0080] The culture system of broiler primary hepatocytes was not treated with T-2 toxin (that is, treated with 0nM T-2 toxin oxidative stress), cultured for 12h, and then added to the culture system of broiler hepatocytes at a concentration of 12μM L-seleno For the methionine working solution, adjust the...

Embodiment 2

[0082] The difference between Example 2 and Example 1 lies in the oxidative stress treatment: the culture system of broiler primary hepatocytes was treated with 10 nM T-2 toxin.

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Abstract

The invention provides a method for applying L-selenomethionine to animal cell antioxidation and a product thereof, which relate to the technical field of animal cell culture. The method comprises thefollowing step of adding an L-selenomethionine working solution into a culture system of animal cells so that the final concentration of L-selenomethionine in the culture system of animal cells is 0.5-1.5 mu M; wherein the concentration of the L-selenomethionine working solution is 5-15 [mu] M. The application method of L-selenomethionine in animal cell antioxidation alleviates the lack of an animal cell antioxidation method which can effectively reduce cell oxidative damage and maintain cell activity, and is low in cytotoxicity, simple and convenient to operate and low in cost in the prior art. The invention also provides an animal cell antioxidant containing the L-selenomethionine, an animal cell culture medium containing the L-selenomethionine or the animal cell antioxidant containingthe L-selenomethionine, and a medicine or a feed additive containing the L-selenomethionine or the animal cell antioxidant containing the L-selenomethionine.

Description

technical field [0001] The invention relates to the technical field of animal cell culture, in particular to a method for the application of L-selenomethionine in the anti-oxidation of animal cells and a product thereof. Background technique [0002] When the animal body or animal cells are stimulated by harmful stimuli in vivo or in vitro, such as the stimulation of some oxidative substances, they can produce oxidative stress. This oxidative stress reaction will cause changes in a series of substances in cells, such as: intracellular valley The content of glutathione (GSH) decreases, the activity of glutathione peroxidase (GPx) decreases, and the level of intracellular ROS increases, which in turn induces the production of intracellular malondialdehyde (MDA), and the level of intracellular MDA increases , Inducing cells to produce oxidative stress, causing oxidative damage to cells, such as increased intercellular space, cell damage, thereby reducing cell activity and even ...

Claims

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Application Information

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IPC IPC(8): C12N5/071A61K31/198A61P39/06A23K20/142
CPCA61K31/198A23K20/142A61P39/06C12N5/067C12N5/0686C12N2500/32
Inventor 杨凌宸吴映欣熊款款王爱兵屠迪刘伟
Owner HUNAN AGRICULTURAL UNIV
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