Method for applying L-selenomethionine to animal cell oxidation resistance and product thereof

Abstract

The invention provides a method for applying L-selenomethionine to animal cell antioxidation and a product thereof, which relate to the technical field of animal cell culture. The method comprises thefollowing step of adding an L-selenomethionine working solution into a culture system of animal cells so that the final concentration of L-selenomethionine in the culture system of animal cells is 0.5-1.5 mu M; wherein the concentration of the L-selenomethionine working solution is 5-15 [mu] M. The application method of L-selenomethionine in animal cell antioxidation alleviates the lack of an animal cell antioxidation method which can effectively reduce cell oxidative damage and maintain cell activity, and is low in cytotoxicity, simple and convenient to operate and low in cost in the prior art. The invention also provides an animal cell antioxidant containing the L-selenomethionine, an animal cell culture medium containing the L-selenomethionine or the animal cell antioxidant containingthe L-selenomethionine, and a medicine or a feed additive containing the L-selenomethionine or the animal cell antioxidant containing the L-selenomethionine.

Description

technical field [0001] The invention relates to the technical field of animal cell culture, in particular to a method for the application of L-selenomethionine in the anti-oxidation of animal cells and a product thereof. Background technique [0002] When the animal body or animal cells are stimulated by harmful stimuli in vivo or in vitro, such as the stimulation of some oxidative substances, they can produce oxidative stress. This oxidative stress reaction will cause changes in a series of substances in cells, such as: intracellular valley The content of glutathione (GSH) decreases, the activity of glutathione peroxidase (GPx) decreases, and the level of intracellular ROS increases, which in turn induces the production of intracellular malondialdehyde (MDA), and the level of intracellular MDA increases , Inducing cells to produce oxidative stress, causing oxidative damage to cells, such as increased intercellular space, cell damage, thereby reducing cell activity and even ...

AI Technical Summary

Problems solved by technology

[0002] When the animal body or animal cells are stimulated by harmful stimuli in vivo or in vitro, such as some oxidative substances, they can produce oxidative stress. This oxidative stress will cause changes in a series of substances in cells, such as: intracellular valley The content of glutathione (GSH) decreases, the activity of glutathione peroxidase (GPx) decreases, and the level of intracellular ROS increases, which in turn induces the production of intracellular malondialdehyde (MDA), and the level of intracellular MDA increases , induce oxidative stress in cells, causing oxidative damage to cells, such as increased intercellular space, cell damage, thereby reducing cell activity and even causing cell death

[0003] At present, antioxidants are widely used to deal with the oxidative damage of animal cells, such as the application of elemental Se, Nac or Vc, etc., but although these antioxidants have strong antioxidative properties, the control of the addition amount must be very strict, and the excessive amount is extremely high. Easy to cause cytotoxicity, resulting in cell damage

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