SNP locus, primer and method for quickly identifying individual gender of takifugu obscurus

Abstract

The invention discloses a SNP locus, a primer and a method for quickly identifying the individual gender of takifugu obscurus, wherein a base sequence of the SNP locus is shown as SEQ ID NO. 1. According to the invention, a male and female-specific SNP locus is obtained by carrying out genome analysis on male and female individuals of three independent genetic populations of the takifugu obscurus,and two pairs of male-specific PCR primers are designed for the locus for identifying the male / female gender of the takifugu obscurus individual; a fin-ray DNA extraction can be completed within 10 minutes by improving a DNA extraction method, with simple and quick operations, and through PCR amplification and agarose gel electrophoresis, the individual with DNA bands is identified as the male, otherwise, the individual is identified as the female, so that distinguishing the male from the female is easy. The method has the characteristics of high speed, high accuracy, simple and convenience in operation, low cost, etc., and the genetic gender identification of the takifugu obscurus can be finished within 2 hours by using the method.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a SNP site, a primer and a method for quickly identifying the individual sex of puffer puffer, which are used for the rapid identification of the genetic sex of puffer puffer. Background technique [0002] Pufferfish is one of the important farmed fishes in China. Pufferfish has white and tender meat, delicious taste and high economic value. It enjoys the reputation of "the king of fish". As the country gradually releases the breeding, processing and sales of redfin puffer and puffer puffer, the puffer fish farming industry has ushered in rapid growth and development. In 2017, the total breeding output has reached 30,686 tons (China Fishery Statistical Yearbook, 2018) . Fugu obscurus is one of the famous "three delicacies of the Yangtze River" in my country. The "white son" (testis) of the male individual is also called "Xi Shi milk". It has a unique flavor and is deeply ...

AI Technical Summary

Problems solved by technology

At present, several publicly available methods for sex identification of puffer fish are almost all developed based on this principle, but there are more or less some shortcomings, such as complicated operation, long time-consuming, high cost, etc.

In the identification of a small number of samples, better identification results can often be obtained, but when large-scale sample identification is carried out, the cumbersome experimental process will undoubtedly greatly increase the labor cost and material cost of the identification, and will increase operational errors (mainly between samples). Mutual contamination), leading to misjudgment of results and increased risk of identification failure

This risk is especially reflected in the DNA extraction stage. Conventional DNA extraction methods involve many steps such as lysis, extraction, precipitation, washing, and dissolution. risk (especially when the sample size is large), and the complicated operation also means that more reagents and consumables are consumed, and the cost is greatly increased

In addition, the previous identification methods often required multiple rounds of PCR reactions or multiple PCR reaction systems, or to distinguish DNA electrophoresis bands of different sizes, which also had problems such as time-consuming, low efficiency, and difficulty in learning.

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