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Method and kit for quantitatively measuring small low-density lipoprotein cholesterol

A low-density lipoprotein, quantitative determination technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., to achieve high reliability, high accuracy, and improve specificity and accuracy

Active Publication Date: 2020-02-14
PUREBIO LAB NINGBO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can measure sd LDL-C more conveniently than electrophoresis or ultracentrifugation, it inevitably requires pretreatment of samples and the separation of LDL into sd LDL and LDL other than sd LDL

Method used

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  • Method and kit for quantitatively measuring small low-density lipoprotein cholesterol
  • Method and kit for quantitatively measuring small low-density lipoprotein cholesterol
  • Method and kit for quantitatively measuring small low-density lipoprotein cholesterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The method for quantitative determination of small dense low-density lipoprotein cholesterol first eliminates other lipoprotein cholesterol under the condition of preferentially protecting LDL-C, and then adds surfactant to selectively quantitatively measure sd LDL-C. For specific testing procedures, see image 3 shown.

[0035] In this method, a kit is required, and the kit used includes two independent liquid reagents, reagent R1 and reagent R2, and the volume ratio of reagent R1 and reagent R2 is 3:1.

[0036] The reagent R1 contains buffer, surfactant, protective agent, cholesterol esterase, cholesterol oxidase, catalase, chromogenic substrate, preservative and anti-interference components.

[0037] The reagent R2 contains a buffer, a surfactant, a peroxidase, a chromogen, and a preservative.

[0038] in,

[0039] The buffer is GOOD'S buffer.

[0040] The surfactant in the reagent R1 is a polyethylene glycol nonionic surfactant polyethylene glycol alkylphenyl et...

Embodiment 2

[0067] The only difference with Example 1 is:

[0068] The preservative in the reagent R1 is a kind of Proclin series, in this embodiment 1, it is Proclin 950 from Sigma Company; the surfactant is nonylphenol polyoxyethylene ether NP-7;

[0069] The surfactant in reagent R2 is polyacrylamide HEAL-06.

[0070] In reagent R1:

[0071] The concentration of the buffer in the solution is 200mmol / L, and the pH of the buffer is 6.0;

[0072] The concentration of surfactant in the solution is 2%;

[0073] The concentration of the protective agent in the solution is 0.05%;

[0074] The concentration of cholesterol esterase in the reagent is 5kU / L;

[0075] The concentration of cholesterol oxidase in the reagent is 1KU / L;

[0076] The concentration of catalase in the reagent is 1500KU / L;

[0077] The concentration of the chromogenic substrate TOOS in the solution is 6mmol / L;

[0078] The concentration of preservative Proclin950 in the solution is 0.1%;

[0079] The concentration...

Embodiment 3

[0089] The only difference with Example 1 is:

[0090] The chromogenic substrate is DAOS.

[0091] The surfactant in reagent R2 is a mixture of alkylbenzene sulfonate HNL006 and polyacrylamide HEAL-06 at a mass ratio of 1:1.

[0092] more specific,

[0093] In reagent R1:

[0094] The concentration of the buffer in the solution is 10mmol / L, and the pH of the buffer is 8.0;

[0095] The concentration of surfactant in the solution is 0.3%;

[0096] The concentration of the protective agent in the solution is 0.1%;

[0097] The concentration of cholesterol esterase in the reagent is 4KU / L;

[0098] The concentration of cholesterol oxidase in the reagent is 0.6KU / L;

[0099] The concentration of catalase in the reagent is 1200KU / L;

[0100] The concentration of the chromogenic substrate in the solution is 2mmol / L;

[0101] The concentration of preservatives in the solution is 0.2%;

[0102] The concentration of the anti-interference component in the solution is 3kU / L.

...

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Abstract

The invention discloses a method for quantitatively measuring small dense low-density lipoprotein cholesterol. The method comprises the following steps of: firstly, under a situation that LDL-C (low-density lipoprotein cholesterol) is preferentially protected, eliminating other lipoprotein cholesterol, and then, adding a surfactant to selectively measuring sd LDL-C. In the method, adopted kits include two independent liquid reagents R1 and R2, wherein the reagent R1 contains a buffer solution, the surfactant, a protective agent, cholesterol esterase, cholesterol oxidase, catalase, a chromogenic substrate, a preservative and an anti-interference ingredient, and the reagent R2 contains a buffer solution, the surfactant, catalase, a color developing agent and a preservative. The kit has the advantages of better performance, high accuracy and specificity, wide linear range and higher cost performance, can effectively resist interference, and provides a good choice for clinic requirements.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for quantitatively measuring small and dense low-density lipoprotein cholesterol. The invention also relates to a kit for quantitatively measuring small and dense low-density lipoprotein cholesterol. Background technique [0002] According to specific gravity, lipoproteins can be roughly divided into chylomicrons (CM), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL) and high-density lipoprotein (HDL). At present, LDL has been widely used clinically to evaluate the risk of arteriosclerosis. However, new studies in recent years have found that LDL is heterogeneous and can be divided into: (1) LDL with low density and large particles (large and buoyant, lb LDL); (2) LDL with high density and small particles Low-density lipoprotein (small low-density lipoprotein cholesterol, sd LDL), referred to as small and dense low-density lipoprotein. It is known that L...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60
CPCC12Q1/60
Inventor 崔贤艳许国和范翠翠
Owner PUREBIO LAB NINGBO
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