A kind of preparation method of active oligopeptide

An oligopeptide and ultrafiltration equipment technology, which can be used in the preparation methods of peptides, chemical instruments and methods, preparations for skin care, etc., and can solve the problems of poor control of enzymatic hydrolysis and low yield of oligopeptides.

Active Publication Date: 2021-09-10
BLOOMAGE BIOTECHNOLOGY CORP LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of poor enzymatic hydrolysis control and low yield of oligopeptides in the current oligopeptide preparation process, the present invention provides a method for preparing antioxidant oligopeptides, which can control the enzymatic hydrolysis process, and the obtained product oligopeptides have high content and high yield , the steps are simple, and the obtained oligopeptide has good antioxidant activity

Method used

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  • A kind of preparation method of active oligopeptide
  • A kind of preparation method of active oligopeptide
  • A kind of preparation method of active oligopeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of embodiment 1 rice protein

[0031] (1) Protein extraction: Take 10kg of broken rice, crush it to 60 mesh, add 100L water and stir evenly, adjust the pH to 13, put it in a water bath at 50°C, stir in the water bath for 3 h, centrifuge at 5000 r / min for 20 min, and keep The supernatant was adjusted to pH 5.5 with 0.1 M HCl, and stood at 4 °C for 30 min to collect protein precipitates, which were freeze-dried and pulverized to obtain coarse rice protein.

[0032] (2) Protein removal: Add water and stir evenly according to the ratio of material to liquid 1:10, adjust the pH to 5.5, place in a water bath at 55 ℃, add 35U / g amylase and 10U / g cellulase according to the amount of crude protein, Stir in a water bath for 3 h, centrifuge at 5000 r / min for 20 min, collect the protein precipitate, and freeze-dry and pulverize to obtain 512.4 g of rice protein, with a protein yield of 5.12%.

Embodiment 2

[0033] Example 2 Preparation of antioxidant oligopeptides

[0034] (1) Take the rice protein prepared in Example 1, add pH 7.5 PBS buffer solution to prepare 10 L of protein solution with a concentration of 10 mg / mL, stir well, put it in an enzymatic hydrolysis reactor at 55°C, and prepare according to the amount of rice protein Add compound protease I comprising 1600 U / g alkaline protease, 2000 U / g trypsin and 2000 U / g neutral protease, and stir for 30 minutes for pre-enzymolysis to obtain a pre-enzymolysis solution;

[0035] (2) Pump the pre-enzymolysis liquid into ultrafiltration equipment 1, enzymolyze at 55°C for 20 minutes, turn on ultrafiltration equipment 1 (Millipore tangential flow plate regenerated cellulose membrane, membrane molecular weight cut-off 5000Da), and obtain permeate 1;

[0036] According to the amount of rice protein, compound protease II including 1500 U / g papain, 1500 U / g neutral protease and 1000 U / g telopeptidase was added to the permeate 1, and th...

Embodiment 3

[0041] Example 3 Preparation of antioxidant oligopeptides

[0042] (1) Take the rice protein prepared in Example 1, add pH 8.0 PB buffer solution to prepare 10 L of protein solution with a concentration of 8 mg / mL, stir well, put it in an enzymatic hydrolysis reactor at 50°C, and prepare according to the amount of rice protein Add compound protease I comprising 1600 U / g alkaline protease, 2000 U / g trypsin and 2000 U / g neutral protease, and stir for 20 minutes for pre-enzymolysis to obtain a pre-enzymolysis solution;

[0043] (2) Pump the pre-enzymolysis liquid into ultrafiltration equipment 1, enzymatically hydrolyze at 50°C for 30 minutes, turn on ultrafiltration equipment 1 (Millipore tangential flow plate regenerated cellulose membrane, membrane molecular weight cut-off 5000Da), and obtain permeate 1;

[0044] According to the amount of rice protein, compound protease II including 1200 U / g papain, 1000 U / g neutral protease and 800 U / g telopeptidase was added to the permeate...

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Abstract

The invention provides a preparation method of oligopeptides: pre-enzymolyze the protein solution; then perform the first filtration: pump the pre-enzymolyzed solution into the ultrafiltration device 1, and obtain the permeate 1 after enzymolysis; the permeate 1 enters Ultrafiltration equipment 2, add compound protease II to obtain permeate 2 after enzymolysis; permeate 2 is subjected to nanofiltration to obtain permeate 3 and effluent, collect effluent (solution containing oligopeptides), and permeate 3 Flow back to the ultrafiltration device 1; then repeat the cycle: continuously replenish the solvent of the protein solution to the ultrafiltration device 1, and continuously obtain the permeate 1, permeate 2, permeate 3 and effluent; the concentration of oligopeptides in the effluent If it is less than the set threshold, pump the pre-enzymatic hydrolysis solution again. The preparation method of the present invention controls the degree of hydrolysis of proteins and polypeptides, extracts oligopeptides to the greatest extent, and improves the utilization rate of raw materials through two kinds of compound proteases and tandem ultrafiltration coupling enzymolysis. The preparation method has simple steps, and the obtained product oligopeptide has high content and high yield, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of oligopeptide preparation, and relates to a method for preparing an antioxidant oligopeptide, in particular to a method for preparing an antioxidant oligopeptide using rice protein as a raw material. Background technique [0002] my country is rich in rice resources, and its output ranks first in the world. The amino acid composition in rice protein is balanced and the ratio is reasonable, which is close to the ideal mode recommended by FAO / WHO. After the rice protein is hydrolyzed into short peptides or amino acids by protease, it can be made into peptide products with higher nutritional value, which can be further used in health care products, beverages, condiments, cosmetics and other industries. About 15% of broken rice is produced during the processing and production of rice. The protein content of broken rice is close to 8%. Its nutritional value is similar to that of rice, but its price is only 1 / 3-1 / 2 of ri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34A23L33/18A61K8/64A61Q19/08
CPCA23V2002/00A61K8/64A61Q19/08A23L33/18C07K1/34C12P21/06A23V2200/302A23V2200/318
Inventor 王静杨桂兰耿凤魏健郭学平刘爱华
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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