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Bionic Janus magnetic-mesoporous silica nanoparticle for specific capture of CTCs, and preparation method and application

A mesoporous silica and nanoparticle technology, applied in the field of nanomaterials, can solve the problem of high technical requirements for CTCs detection, and achieve the effect of reducing non-specific adsorption and large specific surface area.

Active Publication Date: 2020-02-21
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the clinical requirements for the detection of CTCs are higher

Method used

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  • Bionic Janus magnetic-mesoporous silica nanoparticle for specific capture of CTCs, and preparation method and application
  • Bionic Janus magnetic-mesoporous silica nanoparticle for specific capture of CTCs, and preparation method and application
  • Bionic Janus magnetic-mesoporous silica nanoparticle for specific capture of CTCs, and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Provided is a biomimetic Janus magnetic-mesoporous silica nanoparticle for specific capture of CTCs, which includes the Janus magnetic-mesoporous silica nanoparticle and fusion cell membrane coated on its surface.

Embodiment 2

[0029] The preparation method of the biomimetic Janus magnetic-mesoporous silica nanoparticles described in embodiment 1 is provided, comprising the following steps:

[0030] 1) Preparation of carboxylated Janus magnetic-mesoporous silica nanoparticles (Janus Fe 3 o 4 -mSiO 2 -COOH):

[0031] First, the magnetic precursor and polyacrylic acid (PAA) were prepared by high-temperature hydrolysis to obtain polyacrylic acid-modified magnetic nanospheres, and purified;

[0032] Then, using magnetic nanoparticles as the substrate, tetraethylorthosilicate (TEOS) as the silicon source, and cetyltrimethylammonium bromide (CTAB) as the surfactant and template, it was prepared by a sol-gel method. Janus-type magnetic-mesoporous silica nanoparticles;

[0033] Subsequently, the obtained Janus-type magnetic-mesoporous silica nanoparticles were reacted with a silane coupling agent (APS) to prepare aminated nanoparticles, which were modified by carboxylation to prepare negatively charged n...

Embodiment 3

[0044] Performance evaluation of the biomimetic Janus magnetic-mesoporous silica nanoparticles obtained in Example 2

[0045] 1. Reference figure 1 , is a transmission electron microscope picture of bionic Janus magnetic-mesoporous silica nanoparticles, and it can be seen that it has a rod-shaped morphology.

[0046] 2. Carboxylated Janus magnetic-mesoporous silica nanoparticles (Janus Fe 3 o 4 -mSiO 2 -COOH), fused cell membrane (TWM), biomimetic Janus magnetic-mesoporous silica nanoparticles (TWM-Janus Fe 3 o 4 -mSiO 2 ) zeta potential detection analysis: refer to figure 2 As a result of the test, through the characterization of the zeta potential of the material, it was proved that the fused cell membrane was successfully coated on the surface of the nanoparticle.

[0047] 3. Biological safety evaluation:

[0048] Biomimetic Janus magnetic-mesoporous silica nanoparticles (TWM-Janus Fe 3 o 4 -mSiO 2 ) were co-incubated with 4T1 cells for 24h. And set the compari...

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Abstract

The invention discloses a bionic Janus magnetic-mesoporous silica nanoparticle for the specific capture of CTCs, and a preparation method and application thereof. The preparation method comprises thefollowing steps: 1) preparing a carboxylated Janus magnetic-mesoporous silica nanoparticle; 2) preparing a fusion cell membrane; and 3) coating the surface of the Janus magnetic-mesoporous silica nanoparticle with the prepared fusion cell membrane to obtain the bionic Janus magnetic-mesoporous silica nanoparticle. In the bionic Janus magnetic-mesoporous silica nanoparticle, the core, namely the Janus magnetic-mesoporous silica nanoparticle, has an asymmetric structure and a rod-like morphology, and has a large specific surface area; and the surface of the Janus magnetic-mesoporous silica nanoparticle is coated with the fusion cell membrane, the non-specific adsorption of blood cells can be reduced with the help of the characteristic that leukocytes cannot be aggregated in the circulatory system, and thereby the bionic Janus magnetic-mesoporous silica nanoparticle can be well applied to the detection of CTCs.

Description

technical field [0001] The invention relates to the field of nanomaterials, in particular to a biomimetic Janus magnetic-mesoporous silica nanoparticle for specific capture of CTCs, a preparation method and application thereof. Background technique [0002] Cancer has become an important disease that seriously threatens human life and health, and more than 90% of cancer patients die from tumor metastasis. During tumor metastasis, cancer cells detach from the primary tumor focus and enter the blood or lymphatic circulatory system to become circulating tumor cells (CTCs). These CTCs survive in the circulation and further develop into metastatic tumors in distant organs. Therefore, CTCs, as an important mediator between the primary tumor and metastases, are the golden entry point for studying the development process of tumor metastasis, monitoring the curative effect and evaluating the prognosis of patients. However, the abundance of CTCs in peripheral blood is extremely low,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
CPCA61K49/0093A61K49/005
Inventor 王政董文飞常智敏邵丹梅茜李力葛明锋邵昊华尤倩楠
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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