Biomass-based hydrogel adsorbent of immobilized yeast as well as preparation method and application of biomass-based hydrogel adsorbent

A hydrogel and biological matrix technology, applied in biochemical equipment and methods, microbial-based methods, immobilized enzymes, etc., to achieve environmental friendliness, excellent adsorption effect, and easy promotion and use

Pending Publication Date: 2020-02-21
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant report on the biomass-based high

Method used

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  • Biomass-based hydrogel adsorbent of immobilized yeast as well as preparation method and application of biomass-based hydrogel adsorbent
  • Biomass-based hydrogel adsorbent of immobilized yeast as well as preparation method and application of biomass-based hydrogel adsorbent
  • Biomass-based hydrogel adsorbent of immobilized yeast as well as preparation method and application of biomass-based hydrogel adsorbent

Examples

Experimental program
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Effect test

Example Embodiment

[0032] Example 1:

[0033] This embodiment provides a hydrogel including tea residue and Pichia kudder cells.

[0034] The preparation method includes the following steps:

[0035] (1) Preparation of Pichia kudder yeast mud preparation.

[0036] (2) Preparation of tea residue powder aqueous solution.

[0037] (3) Preparation and treatment of tea residue hydrogel.

[0038] Step (1) The specific steps for preparing yeast paste are:

[0039] Inoculate the strains on the slant medium, culture in a constant temperature incubator for 24 hours, take them out, inoculate them into YPD, and expand the culture on a shaker for 24 hours; then take 1 mL of the bacterial solution and add YPD and YPD under NaCl stress. After culturing on a shaker for 24 hours, place the bacterial liquid in a 5mL centrifuge tube and centrifuge at 3700r for 5min to obtain the bacterial sludge. The number of bacteria in the bacterial sludge is 4×10 6 .

[0040] Step (2) The specific steps for preparing the tea dregs powder ...

Example Embodiment

[0046] Example 2: Verification of the hydrogel of Example 1

[0047] 1 Scanning electron microscope results

[0048] Take a small amount of freeze-dried unground TR / PAA hydrogel samples, fix them on a copper plate with conductive glue, and spray gold on the surface of the samples. Use a VEGA3 scanning electron microscope to observe the surface of the sample under 20kV acceleration voltage. Cross-sectional topography to determine its microstructure. The hydrogel swells to make its internal structure contain a large amount of water, which is freeze-dried, and the microstructure and surface morphology of the swelled TR / PAA xerogel (such as figure 1 -d) It can be clearly seen that the TR / PAA xerogel has a three-dimensional porous network structure with inter-connected and irregular pores. According to the scale on the figure, the pore size range can be estimated to be between 100.0 ~ 200.0 μm, which is large 孔结构。 Hole structure. Obviously, the functional groups in this loose, porous s...

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Abstract

The invention discloses biomass-based hydrogel of immobilized yeast and a preparation method of the biomass-based hydrogel. The hydrogel has a three-dimensional macroporous network structure, adsorption sites are fully exposed, water-soluble small molecules can be freely diffused, and the hydrogel is a dual-network hydrogel adsorbent with very high adsorption efficiency on metal ions in an aqueoussolution. According to the invention, microorganisms with an adsorption effect are immobilized into the gel adsorbent, so that the gel adsorbent can fully diffuse water-soluble small molecular substances, has an excellent adsorption effect on heavy metal ions in water, and is environment-friendly, low in cost and convenient to popularize and use.

Description

technical field [0001] The invention relates to the preparation of a biomass-based gel of immobilized yeast and its application as a heavy metal adsorbent, and belongs to the technical fields of environmental functional materials and water pollution treatment. Background technique [0002] Due to the environmental persistence, non-biodegradability, easy bioaccumulation and lethal toxicity of heavy metal pollution in water bodies, it has serious toxic effects on humans and organisms even at relatively low concentrations. In recent years, a variety of treatment technologies have been used to remove toxic metal ion pollutants in wastewater, such as ion exchange, filtration, chemical precipitation, reverse osmosis, redox, electrocoagulation, adsorption, etc. Compared with other methods, the adsorption method has the advantages of high efficiency, low cost, easy operation and implementation, and no secondary pollution. Therefore, the preparation of a low-cost, high-efficiency, a...

Claims

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Application Information

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IPC IPC(8): B01J20/28B01J20/22C12N11/12C12N11/087C12N11/04C08F289/00C08F220/06C08F222/38C02F3/34C12R1/84C02F101/20
CPCB01J20/28047B01J20/22C12N11/12C12N11/08C12N11/04C08F289/00C02F3/34C02F3/347C02F2101/20
Inventor 汪东风张帅中徐莹陈鹏李钰金
Owner OCEAN UNIV OF CHINA
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