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Combined hydrolysis process of bacterial proteins and soybean proteins

A technology of bacterial protein and soybean protein, applied in the biological field, can solve the problems of low degree of hydrolysis, achieve the effects of increased degree of hydrolysis, mild hydrolysis conditions, and mild reaction conditions

Active Publication Date: 2020-02-28
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The degree of hydrolysis obtained in this study is low, and the hydrolysis method cannot be completely applied to hydrolyzed soybean protein

Method used

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  • Combined hydrolysis process of bacterial proteins and soybean proteins
  • Combined hydrolysis process of bacterial proteins and soybean proteins
  • Combined hydrolysis process of bacterial proteins and soybean proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A process for combining hydrolyzed bacterial protein and soybean protein, comprising the steps of:

[0027] The method of obtaining bacterial protein: use a high-speed disk separator to separate the bacterial protein in the threonine fermentation broth, recover the bacterial protein, and dry it.

[0028] Take bacterial protein and soybean protein according to the weight ratio of 2:1, then add citric acid aqueous solution with a concentration of 0.6M according to the ratio of 1g:10ml, stir evenly, and then place it in a high-speed shearing machine at a speed of 10000rpm for 90s, Stand still for 60min, then ultrasonic treatment for 60s, the ultrasonic frequency is 25kHz, and then stand still for 2h, then adjust the pH to 3.0, the temperature is 40°C, then add acid protease, the addition amount is 2000U / g dry matter, and the enzymatic hydrolysis time is 5h , and then adjust the pH to 7.0 and the temperature to 50°C, then add serrapeptase in an amount of 1000U / g dry matter,...

Embodiment 2

[0030] A process for combining hydrolyzed bacterial protein and soybean protein, comprising the steps of:

[0031] Take bacterial protein and soybean protein according to the weight ratio of 3:1, then add a citric acid aqueous solution with a concentration of 0.6M according to the ratio of 1g:8ml, stir evenly, and then place it in a high-speed shearing machine at a speed of 8000rpm for 120s, Stand still for 90 minutes, then ultrasonically treat for 45s, the ultrasonic frequency is 25kHz, and then stand still for 2h, then adjust the pH to 3.0, the temperature is 40°C, then add acid protease, the addition amount is 2000U / g dry matter, and the enzymatic hydrolysis time is 6h , and then adjust the pH to 7.0 and the temperature to 50°C, then add serrapeptase in an amount of 1000U / g dry matter, enzymatic hydrolysis time is 4h, and finally inactivate the enzyme at 100°C for 5min to obtain a hydrolyzate.

Embodiment 3

[0039] Index detection method: Kjeldahl method for determination of total protein; SDS-PAGE for differential determination of protein molecular weight; proteolysis degree determination method uses ninhydrin chromogenic method to determine the degree of hydrolysis.

[0040] 1. The influence of different concentrations of citric acid solution and hydrochloric acid solution on the degree of hydrolysis.

[0041] Set the acid concentration gradient to 0, 0.2, 0.4, 0.6, 0.8, 1.0, the unit is mol / L; for example figure 1 As shown, horizontal observation shows that with the increase of acid concentration, the degree of hydrolysis is also increasing. When the acid concentration reaches 0.6ml / L, the degree of hydrolysis is close to the peak value, and continuing to increase the concentration of acid has no significant impact on the degree of hydrolysis. Considering the There is a certain damage to amino acids, therefore, it is most appropriate to choose a lower concentration of acid. Lon...

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Abstract

The invention belongs to the technical field of organisms, and discloses a combined hydrolysis process of bacterial proteins and soybean proteins. The process includes the steps: taking the bacterialproteins and the soybean proteins according to the ratio of (2-4):1; adding water solution of acid according to the ratio of 1g:(5-10)ml, and uniformly stirring mixture; placing the mixture into a high-speed shearing machine to shear the mixture, standing for 30-90 minutes, ultrasonically treating the mixture, and standing for 2 hours; adjusting pH (potential of hydrogen) to reach 3.0, adjusting temperature to reach 40 DEG C, adding acid protease, and performing enzymatic hydrolysis for 4-6 hours; adjusting the pH to reach 7.0, adjusting the temperature to reach 50 DEG C, adding serrapeptass,and performing enzymatic hydrolysis for 4-6 hours; performing enzyme deactivation to obtain a hydrolysis fluid. The process is suitable for combined hydrolysis of the bacterial proteins and the soybean proteins, and hydrolysis degree and nutritive values are high.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a process for combining hydrolyzed bacterial protein and soybean protein. Background technique [0002] Bacterial protein is a by-product of the process of microbial fermentation to produce amino acids, which is rich in protein and other nutrients. Studies have shown that using cell protein enzymatic hydrolyzate instead of soybean meal hydrolyzate as a nitrogen source to cultivate microorganisms has little effect on microbial fermentation. The applicant accidentally discovered in actual industrial production that a certain proportion of The hydrolyzate of soybean meal can increase the yield of threonine produced by microbial fermentation, but considering that the hydrolyzate of soybean meal contains certain impurities such as pigments, which is not conducive to the subsequent purification of threonine, it can be replaced by soybean protein hydrolyzate. In view of the sim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 王小平程士清时夫龙尤学波赵杰
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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