Method for efficiently expressing hypersensitive protein by using T4 phage display technology

A phage display and hypersensitive protein technology, applied in the field of high-efficiency expression of hypersensitive proteins, can solve the problems of high cost, complicated operation, and many steps.
CN110878320APending Publication Date: 2020-03-13湖北微生元生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
湖北微生元生物科技有限公司
Publication Date
2020-03-13

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Abstract

The invention relates to a method for efficiently expressing hypersensitive protein by using a T4 phage display technology. The method comprises the following steps: (1) sequentially connecting an encoding gene of the hypersensitive protein with TEV protease and a T4 bacteriophage Hoc protein gene segment to prepare a fusion recombinant protein encoding gene, wherein the encoding region of the hypersensitive protein is located at the upstream of a TEV-Hoc fusion protein gene and having consistent expression cassettes; (2) transferring a coding gene of the fusion recombinant protein into a pUC18 plasmid; (3) transferring the plasmid into an escherichia coli host; (4) infecting the Escherichia coli host by using Hoc-T4 bacteriophage deleted by Hoc protein, and culturing the host to express and display the T4 bacteriophage of the fusion recombinant protein; (5) separating the T4 bacteriophage showing the fusion recombinant protein; and (6) separating the fusion recombinant protein from the T4 bacteriophage, and carrying out TEV protease digestion to obtain the hypersensitive protein.
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Description

Technical field

[0001] The present invention belongs to the field of biotechnology, and specifically relates to a method for efficiently expressing hypersensitive proteins using T4 phage display technology. Background technique

[0002] Fertilizers and pesticides are the main products for increasing crop yields and preventing pests and diseases, but at the same time they have caused environmental, energy, and cost problems. They have become major economic and social developments that are now plagued all countries in the world, and seriously restrict the sustainability of agriculture. development of. Biological products (including biological fertilizers and biological pesticides) prepared by using protein from microorganisms or fungi are the main measures for high-quality, high-efficiency and pollution-free production of contemporary crops. In recent years, a lot of scientific research has been carried out in this area at home and abroad. The early microbial proteins were mainly...

Claims

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