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Preparation method of high-purity stachyose

A stachyose and high-purity technology, applied in the field of high-purity stachyose preparation, can solve problems such as difficulty in finding and extracting high-purity stachyose, and achieve the effects of obvious competitive advantages, high purity and complete technology

Inactive Publication Date: 2020-03-24
安徽民祯生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although caddisworms widely exist in Jiangsu, Hebei, Inner Mongolia, Shaanxi and Ningxia, etc. in my country, there are few technical records of extracting stachyose through fresh caddisworms in the prior art, and it is even more difficult to find Technical scheme for extracting high-purity stachyose

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] S1) After washing the fresh caddis worm, bake it at 70°C for 2 hours, then lower the temperature and keep it at 50°C for 24 hours to obtain the dried caddis worm;

[0027] S2) Add water three times the weight of the dried cadaveric product to the dried cadaveric product obtained in S1, extract at 80°C for 20 min, crush the caddis worm, continue leaching for 2 hours, and then filter to obtain the first Filtrate and first filter residue;

[0028] S3) in the first filtrate that S2 makes, add the yeast extract of first filtrate weight 0.2%, the casein of 0.2%, the K of 0.04% 2 HPO 4 and 0.03% calcium carbonate, and cooled to room temperature after high-temperature sterilization to obtain a culture solution;

[0029] S4) Add 1% Aspergillus suspension and 0.1% lactic acid bacteria to the culture solution prepared by S3, wherein the concentration of spores in the Aspergillus suspension is about 160 million / mL. After culturing for 40 hours, heat up to 80°C to inactivate the ...

Embodiment 2

[0037] S1) After washing the fresh caddis worm, bake it at 73°C for 2.5 hours, then lower the temperature and keep it at 53°C for 26 hours to obtain the dried caddis worm;

[0038] S2) Add water 4 times the weight of the dried caddis to the dried caddis produced in S1, extract at 90°C for 25 minutes, crush the caddis, continue to extract for 2.5 hours, and then filter to obtain the first a filtrate and a first filter residue;

[0039] S3) in the first filtrate that S2 makes, add the yeast extract of first filtrate weight 0.4%, the casein peptone of 0.3%, the K of 0.07% 2 HPO 4 and 0.09% calcium carbonate, and cooled to room temperature after high-temperature sterilization to obtain a culture solution;

[0040] S4) Add 2% Aspergillus suspension and 0.3% lactic acid bacteria to the culture solution prepared by S3, wherein the concentration of spores in the Aspergillus suspension is about 170 million / mL. After culturing for 45 hours, heat up to 90°C to inactivate the enzyme fo...

Embodiment 3

[0048] S1) After washing the fresh caddis worm, bake it at 75°C for 3 hours, then lower the temperature and keep it at 55°C for another 30 hours to obtain the dried caddis worm;

[0049] S2) Add water 5 times the weight of the dried cadaveric product to the dried cadaveric product obtained in S1, and extract at 100°C for 30 minutes, crush the cadaveric worm, continue leaching for 3 hours, and then filter to obtain the first Filtrate and first filter residue;

[0050] S3) in the first filtrate that S2 makes, add the yeast extract of first filtrate weight 0.6%, the casein peptone of 0.4%, the K of 0.1% 2 HPO 4 and 0.15% calcium carbonate, and cooled to room temperature after high-temperature sterilization to obtain a culture solution;

[0051] S4) Add 3% Aspergillus suspension and 0.4% lactic acid bacteria by weight to the culture solution prepared in S3, wherein the concentration of spores in the Aspergillus suspension is 180 million / mL, and stand at a constant temperature at...

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Abstract

The invention discloses a preparation method of high-purity stachyose, and belongs to the field of a saccharide production correlation technique. Fresh Chinese artichoke is used as raw materials. Thepreparation method comprises the following steps of preparing dried Chinese artichoke products, performing leaching, performing filtering to prepare first filtrate, preparing a culture solution, performing fermentation to prepare a fermentation solution, performing alcohol leaching, performing filtering to prepare second filtrate, performing alkali precipitation to prepare third filtrate, performing decoloring to prepare fourth filtrate, enabling crystalline particles to be precipitated, and performing vacuum drying to prepare stachyose crystal. According to the scheme, stachyose is directly refined from the fresh Chinese artichoke, the materials are broad to take, the technique is complete, and the production is convenient. The prepared stachyose is high in purity. Compared with like products, the stachyose has more obvious competitive advantages.

Description

technical field [0001] The invention relates to the technical field related to sugar production, in particular to a method for preparing high-purity stachyose. Background technique [0002] Stachyose is a naturally occurring tetrasaccharide, which has a very obvious proliferation effect on beneficial bacteria such as bifidobacteria and lactobacilli in the human gastrointestinal tract, can rapidly improve the environment in the human digestive tract, and regulate the balance of micro-ecological flora. It can promote the formation of beneficial bacteria in the digestive tract as dominant bacteria, inhibit the production of spoilage bacteria such as Clostridium aerogenes, and produce a large number of physiologically active substances, adjust the pH value of the intestinal tract, kill pathogenic bacteria, and prevent spoilage Product generation, inhibit the production and absorption of endogenous carcinogens, and decompose and derive multiple immune function factors. [0003] ...

Claims

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Application Information

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IPC IPC(8): C07H3/06C07H1/08
CPCC07H1/08C07H3/06
Inventor 宋建民王德海宛荣生张琴王颂黄祥君
Owner 安徽民祯生物工程有限公司
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