Blood disease related drug concentration detection method and application

Abstract

The invention relates to a blood disease related drug concentration detection method and application, and the method comprises the following steps: mixing a blood sample to be detected with a cell lysis solution or a buffer salt solution, adding a methanol solution of an isotope internal standard of the drug to be detected, oscillating, carrying out centrifugal separation, and collecting the supernatant; injecting the supernatant into a rapid liquid chromatography system for separation and analysis, then entering a triple quadrupole mass spectrometry detector, collecting and processing data through chromatographic software, and calculating the drug concentration in a blood sample to be detected. According to the blood disease related drug concentration detection method, according to the characteristics and clinical needs of the blood diseases, a scheme for detecting the blood concentration of the medicines related to the blood diseases is established, and the scheme has the advantagesthat a chromatographic column and a mobile phase do not need to be replaced, so that the detection efficiency and the operation convenience are greatly improved, and the method is suitable for large-scale clinical popularization.

Description

Technical field [0001] The invention belongs to the technical field of drug analysis and detection, and specifically relates to a method and application for detecting the concentration of blood disease-related drugs. Background technique [0002] With the introduction of precision medicine and the implementation of the grand health plan, precision medicine is getting more and more attention from the industry and patients. As an important tool for precise medication, blood concentration determination can provide a basis for clinical medication. As a major disease affecting human health, hematological diseases have the characteristics of rapid onset, long cycle, and high mortality rate, which leads to extremely complicated treatment and medication of hematological diseases. In particular, hematological tumors and transplant patients have high toxicity and long medication cycles. Some drugs have large inter-individual and intra-individual differences, and have a small therapeutic w...

AI Technical Summary

Problems solved by technology

As a major disease that affects human health, blood diseases have the characteristics of acute onset, long cycle, and high fatality rate, which makes the treatment and medication of blood diseases extremely complicated.

Especially for patients with hematologic malignancies and transplantation, the drug toxicity is high and the drug cycle is long, and some drugs have large inter-individual and intra-individual differences, and at the same time have a small therapeutic window, which seriously restricts the therapeutic effect of patients.

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