New method for manufacturing paper-based micro-fluidic chip

A microfluidic chip and paper-based technology, applied to chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve the problems of increasing the production cost of paper chips, relying on professional operators, and limiting wide application, etc., to achieve Curing time is controllable, easy to expand, and the effect of reducing production costs

Inactive Publication Date: 2020-04-03
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The above-mentioned paper-based microfluidic chip manufacturing methods depend on the use of professional equipment to varying degrees, such as heating devices, printing devices, exposure devices, cleaning devices, cutting devices, etc. The reliance on professional operators limits the wide application of the above methods in areas where medical resources are scarce, such as field, grassroots, and families

Method used

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  • New method for manufacturing paper-based micro-fluidic chip
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  • New method for manufacturing paper-based micro-fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Evaluation of hydrophobicity of photocurable resin coated on filter paper.

[0033] Use the photocurable resin mixture produced by IKONNA Company to evenly coat on Whatman1# qualitative filter paper, and light it under the ultraviolet LED light source for 3 minutes to polymerize and crosslink the photocurable resin monomer. Then spread the filter paper on the desktop, use a pipette gun to drop distilled water on the surface of the filter paper coated with photocurable resin, take pictures to record the shape of the water drop, and use graphic analysis software to process and calculate the contact angle between the water drop and the surface of the photocurable resin ( figure 1 ), and compared with unmodified filter paper. It is calculated that the contact angle between the water droplet and the surface of the photocurable resin is 112 ± 5°, and the water droplet is completely wetted and absorbed immediately after falling onto the surface of the unmodified fil...

Embodiment 2

[0034] Example 2: Fabrication of a paper-based microfluidic chip using photocurable resin and stamp technology.

[0035] According to the purpose of the chip, design a three-channel microfluidic chip. Engraving and making red plastic seals, the seal pattern is as follows image 3 shown. The side length of the triangle is 3.8 centimeters (cm), the width of the edge protrusions of the three straight channels is 1.5 mm (mm), the width of the edge protrusions of the three detection areas is 1.5 mm, and the width of the edge protrusions of the central sample loading area is 2.25 mm. mm. Use the stamp to print the photocurable resin on the filter paper. The channel size of the formed microfluidic chip is: the width of the three straight channels is 1.5mm, the length is 8mm, the diameter of the central sample area is 3.5mm, and the diameter of the three detection areas 3.5mm. Under the ultraviolet LED light source for 3 minutes, the photocurable resin monomer is polymerized and c...

Embodiment 3

[0036] Example 3: A paper-based microfluidic chip fabricated using photocurable resin and stamp technology is used for the quantitative detection of proteins and heavy metal ions.

[0037] Use bovine serum albumin to prepare a series of protein solutions with concentrations of 6.25, 12.50, 18.75, 25.00, and 31.25 micromole per liter (μM), and use zinc chloride to prepare a series of protein solutions with concentrations of 1, 2, 4, 6, and 8 micrograms per Milliliters (μg / mL) of zinc ion solution. A paper-based microfluidic chip was fabricated using a mixture of qualitative filter paper and photocurable resin to detect the concentration of protein and zinc ions. Add bromophenol blue dropwise to one detection area of ​​the chip, and add zinc ion indicator dropwise to the other detection area of ​​the chip. Add 20 microliters of protein solution dropwise to the sample loading area, use the mobile phone software Color Assist to read the RGB value of the detection area after the r...

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Abstract

The invention relates to a new method for manufacturing a paper-based micro-fluidic chip. According to the method, a chip pattern is directly printed on filtering paper by utilizing a photocuring material and combining a stamp technology while the material is cured by utilizing ultraviolet lamp irradiation so as to generate a hydrophobic solid material on the filtering paper in situ, and the hydrophobic solid material as a hydrophobic dam blocks an aqueous solution flowing through the middle to finally form a patterned hydrophilic channel on the filtering paper so as to obtain the paper-basedmicro-fluidic chip. According to the invention, the manufacturing method has the advantages of low price and easily available raw materials and simple and rapid manufacturing process, can be used formanufacturing a paper-based micro-fluidic chip to achieve on-site analysis and detection, and can also be directly used for manufacturing science popularization teaching aids, implementing classroom experiments and the like.

Description

technical field [0001] The invention relates to the manufacturing technology of a paper-based microfluidic chip. Background technique [0002] Microfluidic chip technology has a wide range of applications in the fields of synthesis and analysis because of its high integration, ease of automation and unique fluid characteristics in micro-channels. Among them, paper-based microfluidic chip technology has the advantages of low cost, high portability, simple steps, and flexible design. It can integrate multiple processing devices such as separation, enrichment, and detection on a single chip to form a "paper laboratory." ", which has been widely used in food safety, drug supervision, environmental governance, health monitoring, medical diagnosis and other fields. [0003] The manufacturing technology of paper-based microfluidic chips has always been one of the research hotspots in this field. The development of simple and easy manufacturing methods is of great significance for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/502707
Inventor 张璐张思哲张书伟
Owner CAPITAL NORMAL UNIVERSITY
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