Preparation method of immobilized microorganism particles for removing ammonia nitrogen in black and odorous water

A technology for immobilizing microorganisms and black and odorous water bodies, applied in chemical instruments and methods, immobilized on or in inorganic carriers, immobilized on/in organic carriers, etc., can solve the problems of low ammonia nitrogen removal efficiency, etc. Enhanced removal performance, reduced production time, and reduced production costs

Pending Publication Date: 2020-04-07
WUHAN UNIV OF TECH
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Problems solved by technology

[0005] In view of this, the present invention aims to propose a method for preparing immobilized microbial particles for removing ammonia nitrogen from black and odorous water bodies, so as to solve the problem of low ammonia nitrogen removal efficiency of existing immobilized bacterial agents

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  • Preparation method of immobilized microorganism particles for removing ammonia nitrogen in black and odorous water
  • Preparation method of immobilized microorganism particles for removing ammonia nitrogen in black and odorous water

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Embodiment 1

[0032] A method for preparing immobilized microbial particles for removing ammonia nitrogen from black and odorous water bodies, specifically comprising the following steps:

[0033] 1) Prepare selection medium, its composition is as follows: 1g / L glucose, 0.1g / L CaCl 2 , 0.3g / L Na 2 HPO 4 , 0.5g / L KH 2 PO 4 , 0.05g / L MgSO 4 , 0.0025g / L MnSO 4 4H 2 O, 0.0025g / L FeSO 4 ·7H 2 O, and the corresponding nutrient solution;

[0034] 2) Prepare solid selection medium, add 20g / L agar powder and 0.02g / L NH 4 Cl, the remaining components are consistent with the selection medium;

[0035] 3) Bacteria enrichment medium was prepared, the components of which were as follows: 5g / L beef extract, 10g / L peptone, 5g / L NaCl;

[0036] 4) Preparation of microbial suspension: take a black and odorous water sample, filter it and add it to the selective medium, add 10 mL of nutrient solution every 2 days, and initially add 2 mL of NH with a concentration of 1000 mg / L 4 Cl solution, after th...

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Abstract

The invention provides a preparation method of immobilized microorganism particles for removing ammonia nitrogen in black and odorous water, which comprises the following steps: 1) grinding zeolite, screening, sterilizing, drying, adding into a microorganism bacterium suspension, oscillating for adsorption, and performing standing for a period of time to obtain a bacterium-liquid mixture; 2) adding the bacterium-liquid mixture into a sodium alginate solution to obtain a mixed solution A; and 3) dropwise adding the mixed solution A into a CaCl2 solution, carrying out a cross-linking reaction, and after the cross-linking reaction is finished, washing with sterile water to obtain the immobilized microorganism particles for removing ammonia nitrogen from the black and odorous water body. The immobilized microorganism particle thalli obtained by the invention are not easy to dissolve out, the particles are not easy to swell, and the immobilized microorganism particle thalli have better material stability and reusability, so that the immobilized microorganism particle thalli have higher ammonia nitrogen removal rate.

Description

technical field [0001] The invention relates to the technical field of preparation of immobilized microbial particles, in particular to a method for preparing immobilized microbial particles used for removing ammonia nitrogen from black and odorous water bodies. Background technique [0002] Nitrogen-containing substances can enter water bodies through natural channels such as precipitation and dustfall and biological nitrogen fixation, and can also enter water bodies through the discharge of sewage and wastewater generated by human activities. Leather industry, pesticides and nitrogen fertilizers, livestock and poultry breeding wastewater are all sources of ammonia nitrogen pollution in water bodies. Ammonia nitrogen is an oxygen-consuming pollutant. When its concentration is too high, the dissolved oxygen in the water body will be greatly reduced, which will affect the survival of aquatic animals such as fish and shrimp, and then make the water body black and smelly. In s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04C02F3/34C02F101/16
CPCC12N11/14C12N11/10C12N11/04C02F3/34C02F2101/16
Inventor 黄永炳吴伟珊谢依廷李瑶闫萌萌余少波
Owner WUHAN UNIV OF TECH
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