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Antioxidant polypeptide and preparation method thereof

A technology for antioxidant polypeptides and seeds, applied in the field of bioengineering, can solve problems such as cost reduction, inability to achieve, and complex preparation methods

Pending Publication Date: 2020-04-07
南京平港生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides an anti-oxidative polypeptide and its preparation method, which solves the problem that the existing preparation method is relatively complicated and cannot realize the concentrated fermentation and enzymolysis treatment of various proteins, saves production costs, and cannot achieve the goal of passing Microbial fermentation and enzymatic hydrolysis will be carried out at the same time to reduce costs, and the terminal peptidase produced by microorganisms will not be able to modify the end of the peptide, so that the bitterness of the prepared peptide will disappear and the problem of natural aroma will be emitted.

Method used

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  • Antioxidant polypeptide and preparation method thereof

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Effect test

Embodiment 1

[0027] S1. Fermentation and enzymatic hydrolysis treatment: firstly, 7 parts of animal serum albumin, 7 parts of zein, 7 parts of ovalbumin, 7 parts of wheat prolamin, 15 parts of casein, 15 parts of oilseed protein and 15 parts of soybean were selected Pour the protein into the fermenter respectively, then add 35mL of warm water, then inoculate the fermentation strain, start the heating equipment, control the temperature of the fermenter at 30°C, and adjust the pH within the range of 6, then add 16mL of hydrolyzate to the fermenter The protease was stirred for 25 minutes by a stirring device at a speed of 250r / min. After the mixing was completed, the fermenter was sealed and fermented for 14 hours. The oilseed proteins were peanut seed protein, sesame seed protein, rapeseed The composition of flower seed protein and coconut seed protein, animal serum albumin is the composition of porcine serum albumin, sheep serum albumin and bovine serum albumin;

[0028] S2. Filtration trea...

Embodiment 2

[0032]S1. Fermentation and enzymatic hydrolysis treatment: firstly, the selected 5 parts of animal serum albumin, 5 parts of zein, 5 parts of ovalbumin, 5 parts of wheat prolamin, 20 parts of casein, 20 parts of oilseed protein and 20 parts of soybean Pour the protein into the fermenter respectively, then add 30mL of warm water, then inoculate the fermentation strain, start the heating equipment, control the temperature of the fermenter at 26°C, and adjust the pH within the range of 5, then add 12mL of hydrolyzate to the fermenter The protease was stirred by a stirring device at a speed of 200r / min for 20 minutes. After the mixing was completed, the fermenter was sealed and fermented for 12 hours. The oilseed protein was peanut seed protein, and the animal serum albumin was porcine serum albumin;

[0033] S2. Filtration treatment: transfer the material after the fermentation in step S1 to a filtration device, perform filtration treatment through an ultrafiltration membrane, fil...

Embodiment 3

[0037] S1. Fermentation and enzymatic hydrolysis treatment: first, 10 parts of animal serum albumin, 10 parts of zein, 10 parts of ovalbumin, 10 parts of wheat prolamin, 10 parts of casein, 10 parts of oilseed protein and 10 parts of soybean were selected Pour the protein into the fermenter respectively, then add 40mL of warm water, then inoculate the fermentation strain, start the heating equipment, control the temperature of the fermenter at 33°C, and adjust the pH within the range of 7, then add 20mL of hydrolyzate to the fermenter The protease was stirred for 30 minutes by a stirring device at a speed of 300 r / min. After the mixing was completed, the fermenter was sealed and fermented for 16 hours. The oil seed protein was coconut seed protein, and the animal serum albumin was bovine serum albumin;

[0038] S2. Filtration treatment: transfer the material after the fermentation in step S1 to a filtration device, perform filtration treatment through an ultrafiltration membran...

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Abstract

The invention discloses an antioxidant polypeptide and a preparation method thereof. The polypeptide is obtained by carrying out production and processing by using animal serum albumin, corn protein,ovalbumin, wheat gliadin, casein, oil seed protein and soybean protein. The oil seed protein is one or a combination of more of peanut seed protein, sesame seed protein, rapeseed seed protein, sunflower seed protein, cottonseed seed protein, safflower seed protein and coconut seed protein. The invention relates to the technical field of bioengineering. According to the antioxidant polypeptide andthe preparation method thereof, several kinds of proteins can be subjected to centralized fermentation and enzymolysis treatment; the production cost is saved and the preparation process is simple; the objective of reducing the cost by simultaneously carrying out microbial fermentation and enzyme hydrolysis processes is well achieved; and the peptide tail end is modified by terminal peptidase generated by microorganisms, so that the bitter taste of the prepared peptide disappears and the natural fragrance is emitted.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to an antioxidant polypeptide and a preparation method. Background technique [0002] Since the British scientist Harman proposed the theory of free radicals in 1956, with the deepening of research, people have a deeper understanding of the aging of the body caused by excessive free radicals and the induction of various diseases, and the intake of highly active antioxidants Intake is an effective way to remove excess free radicals in the body. At present, chemical antioxidants are the most produced, but their use is limited due to obvious toxic and side effects. With the improvement of people's food safety and antioxidant safety requirements, people Began to turn our attention to natural antioxidants. Compared with chemical antioxidants, natural antioxidants are much safer than the former, so more and more people pay attention to them. In the research on natural antioxidants,...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16
CPCC12P21/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16
Inventor 应巧红张昱澜
Owner 南京平港生物技术有限公司
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