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Application of ferulic acid in preparation of medicine for inhibiting chromium-induced lung inflammatory injury

A technology of inflammatory injury and ferulic acid, which can be used in drug combinations, antipyretics, anti-inflammatory agents, etc., to solve problems such as unclear protective effects

Pending Publication Date: 2020-04-17
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have pointed out that ferulic acid, one of the polyphenolic compounds, has beneficial effects on liver, nerve, and cardiovascular and cerebrovascular diseases, but the protective effect of ferulic acid on chromium-induced inflammatory injury of BEAS-2B cells has not yet been clarified

Method used

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  • Application of ferulic acid in preparation of medicine for inhibiting chromium-induced lung inflammatory injury
  • Application of ferulic acid in preparation of medicine for inhibiting chromium-induced lung inflammatory injury
  • Application of ferulic acid in preparation of medicine for inhibiting chromium-induced lung inflammatory injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1B

[0024] Example 1BEAS-2B cell survival quantity detection

[0025] BEAS-2B cells in the logarithmic growth phase were inoculated in 96-well plates, and the cells were divided into groups according to the experimental requirements: blank control group, K 2 Cr 2 o 7 (2.5μM) injury group, three concentrations of ferulic acid (25μM, 50μM and 100μM) protection group. Add 2.5 μM K alone to the injury group 2 Cr 2 o 7 After treatment for 24 hours, in the protection group, three concentrations of ferulic acid were added for pre-protection for 3 hours and then 2.5 μM K 2 Cr 2 o 7 Co-incubate for another 24 hours. Add 20 μL of MTT solution to each well after treatment in 5% CO 2 , Continue culturing for 4 hours in a 37°C cell culture incubator. After staining, the culture medium was aspirated and 150 μL of DMSO solution was added to each well, and placed on a shaker for 10 minutes. After the formazan crystals were completely dissolved, the absorbance at 490 nm was detected with...

Embodiment 2

[0027] Example 2 Detection of NLRP3 Inflammasome Assembly and Activation

[0028] Example 1 above shows that ferulic acid can protect BEAS-2B cells from chromium-induced decrease in cell viability. At the same time, research on its protective mechanism is necessary. Consisting of NOD-like receptors (NLRs), apoptosis-associated speck-like protein (ASC), and caspase-1, the NLRP3 inflammasome is the most important intracellular pattern One of the recognition receptors. The NLRP3 inflammasome can sense exogenous and endogenous "danger signals" and induce inflammation. After the NLRP3 inflammasome is activated, it can hydrolyze IL-1β precursor (pro-IL-1β) through caspase-1 to form active IL-1β, and then mediate the occurrence of inflammation.

[0029] BEAS-2B cells in the logarithmic growth phase were treated and grouped, and the grouping of cells was the blank control group, K 2 Cr 2 o 7 (2.5μM) injury group, three concentrations of ferulic acid (25μM, 50μM and 100μM) protec...

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Abstract

The invention discloses an application of ferulic acid in the preparation of a medicine for preventing and treating lung inflammation. A new possible medicine is provided for preventing and treating inflammatory injury suffered by lungs, and a certain experimental basis is provided for development and function research of natural polyphenol compound resources. The ferulic acid can be used for preventing and treating lung cell injury caused by chromium pollution, assembling NLRP3 inflammatory corpuscles and expressing cell inflammatory factors so as to play a protective role. Ferulic acid is aphenolic compound, has a wide plant source, exists in rice, apples, peanuts and coffee, has various biological activities and has potential of deep development.

Description

technical field [0001] The invention relates to the application of ferulic acid, in particular to the application of ferulic acid in the preparation of medicines for inhibiting chromium-induced pulmonary inflammatory injury. Background technique [0002] Chromium exists widely in nature, and its natural source is mainly rock weathering. The source of man-made pollution is the mining of chromium, the discharge of industrial chromium-containing waste gas and wastewater, and the consumption of chromium-containing products. Chromium is currently the major environmental pollutant in more than half of all hazardous waste sites, and its many chemical forms have serious impacts on the environment and human health. Hexavalent chromium (Cr(VI)) is considered the most toxic type of chromium and is usually combined with oxygen to form chromate (CrO 4 2- ) or dichromate (Cr 2 o 7 2- ) Oxygen anions. [0003] The lung is an important organ of the human body and a part of the respira...

Claims

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Application Information

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IPC IPC(8): A61K31/192A61P11/00A61P29/00
CPCA61K31/192A61P11/00A61P29/00
Inventor 刘剑利田思琪刘诗迪辛充毕若尘赵艺淑王高佳
Owner LIAONING UNIVERSITY
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