A kind of thermophilic deep-sea microbacterium and its application

Abstract

The invention provides a thermophilic deep-sea microbacterium, whose preservation number is CGMCC No. 18990, and the deep-sea microbacterium P-47-3 strain provided by the invention is used for fermenting fish paste. The strain P-47-3 used in the present invention is isolated from the extreme environment of deep-sea hydrothermal fluids, produces spores, has a growth temperature range of 10°C-65°C, and can grow and reproduce at a salt concentration of 0-14%, while general high-temperature bacteria mainly It comes from terrestrial environments such as hot springs, has poor salt tolerance, and cannot grow under high-salt conditions. Fermentation of marine biological products has great limitations, and the halophilic strain P‑47‑3 is not only suitable for fermenting marine organisms, but also The metabolic process will consume salt, further reducing the salt concentration in the fermented product and improving the quality of the fermented product.

Description

technical field [0001] The invention belongs to the technical field of microbial screening application, and in particular relates to a thermophilic deep-sea microbacterium and its application. Background technique [0002] Fish meal is currently the main protein source in aquafeeds because of its high protein content, various essential amino acids, and easy absorption. China is a big aquaculture country in the world. In 2018, the total aquaculture output exceeded 50 million tons, accounting for more than 78% of my country's total aquatic product output. China is the country with the largest consumption of fishmeal. China's aquaculture industry consumes about 2.5 million tons of fishmeal (partially used for poultry farming) every year, of which 1.2 million tons are imported fishmeal and 1.3 million tons are domestic fishmeal. It can be seen that China's aquaculture industry relies on fishmeal resources and is also restricted by foreign fishmeal imports. [0003] In the pro...

AI Technical Summary

Problems solved by technology

(1) In the preparation process of enzymatic fish paste, only endogenous enzymes are used, and the enzymatic hydrolysis is not complete; the addition of commercial enzymes such as protease increases the cost; after the enzymatic hydrolysis reaction, a high temperature treatment of about 90 ° C is required to destroy the enzyme, which will destroy the anchovy Functional active substances; enzymatic hydrolysis products have poor fluidity and are easy to block pipelines

(2) In the preparation process of ordinary fermented fish paste, the fermented strains are generally from land sources and are used to ferment marine organisms, which are relatively poor in salt tolerance

At present, the thermoprotease-producing strains generally come from land hot springs and other environments. Due to the particularity of marine high-temperature environment sampling, the thermoprotease-producing strains isolated from the deep-sea high-temperature environment are used to ferment anchovy. There is no relevant report so far.

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