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Method for extracting flavonoid compounds from wild rice by using deep eutectic solvent

A deep eutectic solvent and medium flavonoid technology, which can be used in the fields of antidote, organic chemistry, drug combination, etc., can solve the problems of toxic organic solvents, low vapor pressure, easy volatility, etc., to promote dissolution, strong polarity, and low detection limit. Effect

Active Publication Date: 2020-04-21
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the extraction process, organic solvents are toxic, have low vapor pressure, are volatile, and are flammable, and are likely to cause pollution to the environment.

Method used

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  • Method for extracting flavonoid compounds from wild rice by using deep eutectic solvent
  • Method for extracting flavonoid compounds from wild rice by using deep eutectic solvent
  • Method for extracting flavonoid compounds from wild rice by using deep eutectic solvent

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preparation example Construction

[0041] In the present invention, the preparation method of the deep eutectic solvent preferably includes the following steps: first mixing the choline chloride and the hydrogen bond donor, and then adding water for the second mixing to obtain the deep eutectic solvent. In the present invention, the method of the first mixing and the second mixing is preferably stirring and mixing; the rotating speed of the stirring and mixing is preferably 200-800 rpm, more preferably 350-550 rpm; the temperature of the first mixing is preferably 45 ~80°C, more preferably 60~80°C, most preferably 80°C; the first mixing time is preferably 30~360min, more preferably 60~240min; the second mixing temperature is preferably 25~35 °C, the time is preferably 10 to 60 minutes. In the present invention, the deep eutectic solvent is a stable, uniform and transparent liquid. The deep eutectic solvent provided by the invention is an extractant with strong polarity, which can effectively break the wild ric...

Embodiment 1

[0056] (1) Preparation of deep eutectic solvent

[0057] Add choline chloride and 1,4-butanediol into a round-bottomed flask at a molar ratio of 1:6, add magnets into the round-bottomed flask, and use an IKA magnetic stirrer to magnetically stir at 80°C for 60 minutes. The choline chloride-1,4-butanediol mixed solution was obtained, and 30 wt% of water was added to the obtained mixed solution to obtain a deep eutectic solvent (abbreviated as DES-1).

[0058] (2) Wild rice pretreatment

[0059] Grind wild rice with a pulverizer, dry at 40°C until constant weight, and store wild rice powder at -20°C in the dark for future use.

[0060] (3) Analysis of wild rice powder

[0061] Use BSA124S-CW electronic balance to weigh 500 mg of wild rice powder, add 10 mL of deep eutectic solvent and mix well, place it in a KQ-500GVDV double-combined constant-temperature numerical control ultrasonic generator, at 50 ° C, ultrasonic power 200 W, ultrasonic frequency Under the condition of 25k...

Embodiment 2～3

[0080] The flavonoids are extracted and detected according to the method of Example 1. The difference from Example 1 is that the deep eutectic solvent is different. The composition of the deep eutectic solvent in Examples 1 to 3 is as shown in Table 3. Catechin, proanthocyanidin and quercetin The extraction amounts of the three flavonoids of corticosteroids are shown in Table 4.

[0081] Deep eutectic solvent composition in table 3 embodiment 1~7

[0082]

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Abstract

The invention relates to the technical field of natural substance extraction, in particular to a method for extracting flavonoid compounds in wild rice by using a deep eutectic solvent. The inventionprovides a method for extracting flavonoid compounds from wild rice by using a deep eutectic solvent. The method comprises the following steps: pretreating wild rice to obtain wild rice powder, extracting the wild rice powder by using a deep eutectic solvent, then carrying out solid-liquid separation to obtain a flavone extracting solution, and carrying out liquid chromatography separation on theflavone extracting solution to obtain a flavone compound, wherein the deep eutectic solvent comprises choline chloride, a hydrogen bond donor and water and the hydrogen bond donor comprises 1, 4-butanediol, glycerol or lactic acid. The deep eutectic solvent adopted by the method is non-toxic, biodegradable and environment-friendly, and can be recycled, so that the extraction cost is greatly reduced. The method provided by the invention is high in extraction efficiency of flavonoid compounds in wild rice and low in detection limit.

Description

technical field [0001] The invention relates to the technical field of natural substance extraction, in particular to a method for extracting flavonoids in wild rice by using a deep eutectic solvent. Background technique [0002] Wild rice is the caryopsis of Chinese wild rice in the Oryza subfamily of Gramineae. It is a nutritious whole grain and has been eaten in China for at least 3,500 years. It mainly grows in submerged swamps, ponds, swamps, paddy fields and shallow lakes on the edge of lakes; it is more distributed in northeast, north, central, south, southwest, and south-central China. Wild rice contains various phytochemical components such as proteins, minerals, vitamins, phenols, saponins, phytosterols and anthocyanins. Moreover, wild rice has rich biologically active functions, including anti-oxidation, anti-inflammation, anti-obesity and anti-allergic effects, which has attracted extensive attention of domestic and foreign researchers. [0003] At present, fla...

Claims

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Application Information

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IPC IPC(8): C07D311/62C07D311/30C07D311/40A61K36/899A61P3/10A61P35/00A61P39/06
CPCA61K36/899A61K2236/333A61P3/10A61P35/00A61P39/06C07D311/30C07D311/40C07D311/62
Inventor 谭家能窦玉青张忠锋闫宁刘新民
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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