Method for extracting flavonoid compounds from wild rice by using deep eutectic solvent

A deep eutectic solvent and medium flavonoid technology, which can be used in the fields of antidote, organic chemistry, drug combination, etc., can solve the problems of toxic organic solvents, low vapor pressure, easy volatility, etc., to promote dissolution, strong polarity, and low detection limit. Effect

Active Publication Date: 2020-04-21
TOBACCO RES INST CHIN AGRI SCI ACAD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the extraction process, organic solvents are toxic, have low vapor pressu

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting flavonoid compounds from wild rice by using deep eutectic solvent
  • Method for extracting flavonoid compounds from wild rice by using deep eutectic solvent
  • Method for extracting flavonoid compounds from wild rice by using deep eutectic solvent

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0041] In the present invention, the preparation method of the deep eutectic solvent preferably includes the following steps: first mixing choline chloride and hydrogen bond donor, and then adding water for second mixing to obtain the deep eutectic solvent. In the present invention, the method of the first mixing and the second mixing is preferably stirring and mixing; the speed of the stirring and mixing is preferably 200 to 800 rpm, more preferably 350 to 550 rpm; the temperature of the first mixing is preferably 45 ~80℃, more preferably 60~80℃, most preferably 80℃; the time of the first mixing is preferably 30~360min, more preferably 60~240min; the temperature of the second mixing is preferably 25~35 ℃, the time is preferably 10-60 min. In the present invention, the deep eutectic solvent is a stable, uniform and transparent liquid. The deep eutectic solvent provided by the present invention is an extractant with strong polarity, which can efficiently break the cell wall of ...

Example Embodiment

[0055] Example 1

[0056] (1) Preparation of deep eutectic solvent

[0057] Add choline chloride and 1,4-butanediol into a round-bottom flask at a molar ratio of 1:6, add a magnet to the round-bottom flask, and use an IKA magnetic stirrer to magnetically stir at 80°C for 60 minutes. A mixed solution of choline chloride-1,4-butanediol is obtained, and 30 wt% of water is added to the obtained mixed solution to obtain a deep eutectic solvent (abbreviated as DES-1).

[0058] (2) Wild rice pretreatment

[0059] The wild rice is ground with a pulverizer, dried at 40°C until constant weight, and the obtained wild rice powder is stored at -20°C and protected from light for later use.

[0060] (3) Analysis of wild rice powder

[0061] Use BSA124S-CW electronic balance to weigh 500mg of wild rice powder, add 10mL deep eutectic solvent to mix thoroughly, and place it in KQ-500GVDV Shuangpin constant temperature numerical control ultrasonic generator, at 50℃, ultrasonic power is 200W, ultrasonic fr...

Example

[0079] Examples 2~3

[0080] The flavonoids were extracted and detected according to the method of Example 1. The difference from Example 1 is that the deep eutectic solvent is different. The composition of the deep eutectic solvent in Examples 1 to 3 is shown in Table 3. Catechin, proanthocyanidin and quercetin The extraction amounts of the three flavonoids of cortexin are shown in Table 4.

[0081] Table 3 The composition of deep eutectic solvents in Examples 1-7

[0082]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of natural substance extraction, in particular to a method for extracting flavonoid compounds in wild rice by using a deep eutectic solvent. The inventionprovides a method for extracting flavonoid compounds from wild rice by using a deep eutectic solvent. The method comprises the following steps: pretreating wild rice to obtain wild rice powder, extracting the wild rice powder by using a deep eutectic solvent, then carrying out solid-liquid separation to obtain a flavone extracting solution, and carrying out liquid chromatography separation on theflavone extracting solution to obtain a flavone compound, wherein the deep eutectic solvent comprises choline chloride, a hydrogen bond donor and water and the hydrogen bond donor comprises 1, 4-butanediol, glycerol or lactic acid. The deep eutectic solvent adopted by the method is non-toxic, biodegradable and environment-friendly, and can be recycled, so that the extraction cost is greatly reduced. The method provided by the invention is high in extraction efficiency of flavonoid compounds in wild rice and low in detection limit.

Description

technical field [0001] The invention relates to the technical field of natural substance extraction, in particular to a method for extracting flavonoids in wild rice by using a deep eutectic solvent. Background technique [0002] Wild rice is the caryopsis of Chinese wild rice in the Oryza subfamily of Gramineae. It is a nutritious whole grain and has been eaten in China for at least 3,500 years. It mainly grows in submerged swamps, ponds, swamps, paddy fields and shallow lakes on the edge of lakes; it is more distributed in northeast, north, central, south, southwest, and south-central China. Wild rice contains various phytochemical components such as proteins, minerals, vitamins, phenols, saponins, phytosterols and anthocyanins. Moreover, wild rice has rich biologically active functions, including anti-oxidation, anti-inflammation, anti-obesity and anti-allergic effects, which has attracted extensive attention of domestic and foreign researchers. [0003] At present, fla...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D311/62C07D311/30C07D311/40A61K36/899A61P3/10A61P35/00A61P39/06
CPCA61K36/899A61K2236/333A61P3/10A61P35/00A61P39/06C07D311/30C07D311/40C07D311/62
Inventor 谭家能窦玉青张忠锋闫宁刘新民
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap