Method for extracting flavonoid compounds from wild rice by using deep eutectic solvent
A deep eutectic solvent and medium flavonoid technology, which can be used in the fields of antidote, organic chemistry, drug combination, etc., can solve the problems of toxic organic solvents, low vapor pressure, easy volatility, etc., to promote dissolution, strong polarity, and low detection limit. Effect
Active Publication Date: 2020-04-21
TOBACCO RES INST CHIN AGRI SCI ACAD
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The invention relates to the technical field of natural substance extraction, in particular to a method for extracting flavonoid compounds in wild rice by using a deep eutectic solvent. The inventionprovides a method for extracting flavonoid compounds from wild rice by using a deep eutectic solvent. The method comprises the following steps: pretreating wild rice to obtain wild rice powder, extracting the wild rice powder by using a deep eutectic solvent, then carrying out solid-liquid separation to obtain a flavone extracting solution, and carrying out liquid chromatography separation on theflavone extracting solution to obtain a flavone compound, wherein the deep eutectic solvent comprises choline chloride, a hydrogen bond donor and water and the hydrogen bond donor comprises 1, 4-butanediol, glycerol or lactic acid. The deep eutectic solvent adopted by the method is non-toxic, biodegradable and environment-friendly, and can be recycled, so that the extraction cost is greatly reduced. The method provided by the invention is high in extraction efficiency of flavonoid compounds in wild rice and low in detection limit.
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