A Strain Hippocampus Pathogenic Strain and Epidermal Ulcer Syndrome Inactivated Vaccine
A technology for inactivating vaccines and pathogenic bacteria, applied in vaccines, veterinary vaccines, bacteria, etc., can solve problems such as drug residues and drug resistance, and achieve the effects of low cost, stable output, and simple preparation process
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0015] Example 1 Information of Vibrio harveyi CGMCC No.15442 screened by the present invention
[0016] 1) Screening of Vibrio harveyi
[0017] The diseased striated hippocampus with typical symptoms was obtained from Qingdao Qingyuan Marine Biotechnology Company. The diseased hippocampus was washed three times with 0.01mol / L sterile phosphate-buffered saline (PBS), and then the diseased hippocampus was treated. Dissect, use sterile scissors to collect the festered muscle and intestinal tissue in a centrifuge tube filled with sterilized PBS buffer, cut the tissue into pieces, suspend in PBS buffer, take the supernatant to dilute and spread on The 2216E culture plate and TCBS culture plate were cultured in a 28°C incubator for 24 hours, and the dominant colonies with the same morphological characteristics were picked for isolation and purification. The strains purified four times were subjected to routine physiological and biochemical and 16S rDNA gene sequence determinations,...
Embodiment 2
[0027] The preparation of the inactivated vaccine of embodiment 2 Vibrio harveyi CGMCC No.15442
[0028] 1) First, the Vibrio harveii screened by the present invention is inoculated in 1000ml high-salt common LB liquid medium (tryptone 10g / L, yeast extract juice 5g / L, sodium chloride 30g / L), at 28 ℃ Shake culture for 12 hours, and the concentration of the bacterial liquid detected by the hemocytometer reaches 10 9 When the CFU / ml is reached, the culture is terminated to obtain the expanded culture solution of Vibrio harveyi;
[0029] 2) add 50ml formaldehyde solution by volume ratio 0.5% afterward, inactivate the expanded culture solution of bacterial strain at room temperature for 12 hours, shake 5 times during this period; Obtain the inactivated bacterial solution of bacterial strain Vibrio harveii;
[0030] 3) Finally, centrifuge the inactivated bacterial solution of the bacterial strain at 8000g and 4°C for 15 minutes, discard the supernatant, wash the precipitate twice w...
Embodiment 3
[0032] The immune protection test 1 of embodiment 3 vaccine
[0033] The immune effect experiment was carried out on Japanese hippocampus with the prepared inactivated vaccine. Randomly extract 100 Japanese hippocampi with a body length of 2-3 cm, randomly divide them into two treatment groups, use the inactivated vaccine prepared in Example 2 to carry out soaking immunization test, the first group is the control group, and the second group is the immunization test. group, the second group of Japanese hippocampus was placed in 8% NaCl solution and soaked for 2 minutes, and then immediately transferred to the 10-fold diluted inactivated bacteria solution (2×10 8 Soak in CFU / m1) for 3 minutes, and after 28 days, the Japanese hippocampus of the two groups was challenged. The hippocampus of the control group died at 48 hours. The hippocampus of the second group of vaccine treatment group began to die at 33 hours after soaking and challenged, and a total of 29 animals died within 4...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More