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Method of preparing fermented coffee by using intestinal bacteria of cats

A technology of intestinal bacteria and intestinal flora, which is applied in the treatment of roasted coffee, biochemical equipment and methods, microorganisms, etc., can solve the problems of high price, low caffeine concentration, and difficulty in the production of cat feces coffee. Low, pleasant mood, weak bitterness effect

Inactive Publication Date: 2020-04-28
肽晟堂生物科技(常州)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In order to overcome the disadvantages of difficult and expensive production of cat feces coffee, the present invention provides a method for producing fermented coffee by using cat feces as raw material to separate, purify and identify feline intestinal flora , the amplified DNA of the purified strains was obtained by PCR amplification technology and sequenced, compared with the database sequence, and the strains to be isolated were further determined, and the strains were identified as A, B, C and D respectively. Fermentation and vacuum treatment are carried out after inoculating coffee beans in proportion, so that the prepared coffee has a high content of tryptophan, a low concentration of caffeine, a normal color, and a unique aroma

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  • Method of preparing fermented coffee by using intestinal bacteria of cats

Examples

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specific Embodiment 1

[0044] Specific Example 1: (1) Isolation and purification of the intestinal flora of civet cats: 10 g of feces and 100 mL of sterile water were put into a homogenization bag, beat for 5 minutes with a homogenizer, centrifuged in a high-speed refrigerated centrifuge, and removed the supernatant , to make a homogenate; identification: first prepare the identification medium of enteropathogenic bacteria (peptone 17.2g, tryptophan 0.9g, Hepes buffer 0.4g, mixed color source 6.87g, agar 18g, purified water 1L, pH 7.3 ) to identify whether there are pathogenic bacteria in the intestinal flora, if there are no pathogenic bacteria, the following experiments are carried out; preparation medium: prepare beef extract peptone medium, Sabouraud dextrose peptone agar, yeast enrichment medium , Eosin methylene blue lactose medium, and Hektoen medium, about 500mL each, aliquoted, sterilized at 121°C for 15 minutes; pour plate: beef extract peptone medium, Sabouraud dextrose peptone agar, yeast...

specific Embodiment 2

[0045] Specific Example 2: (1) Isolation and purification of the intestinal flora of civet cats: 10 g of feces and 100 mL of sterile water were put into a homogenate bag, beat for 5 minutes with a homogenizer, centrifuged in a high-speed refrigerated centrifuge, and removed the supernatant , to make a homogenate; identification: first prepare the identification medium of enteropathogenic bacteria (peptone 17.2g, tryptophan 0.9g, Hepes buffer 0.4g, mixed color source 6.87g, agar 18g, purified water 1L, pH 7.3 ) to identify whether there are pathogenic bacteria in the intestinal flora, if there are no pathogenic bacteria, the following experiments are carried out; preparation medium: prepare beef extract peptone medium, Sabouraud dextrose peptone agar, yeast enrichment medium , Eosin methylene blue lactose medium, and Hektoen medium, about 500mL each, aliquoted, sterilized at 121°C for 15 minutes; pour plate: beef extract peptone medium, Sabouraud dextrose peptone agar, yeast enr...

specific Embodiment 3

[0046]Specific Example 3: (1) Isolation and purification of the intestinal flora of civet cats: 10 g of feces and 100 mL of sterile water were put into a homogenization bag, beat for 5 minutes with a homogenizer, centrifuged in a high-speed refrigerated centrifuge, and removed the supernatant , to make a homogenate; identification: first prepare the identification medium of enteropathogenic bacteria (peptone 17.2g, tryptophan 0.9g, Hepes buffer 0.4g, mixed color source 6.87g, agar 18g, purified water 1L, pH 7.3 ) to identify whether there are pathogenic bacteria in the intestinal flora, if there are no pathogenic bacteria, the following experiments are carried out; preparation medium: prepare beef extract peptone medium, Sabouraud dextrose peptone agar, yeast enrichment medium , Eosin methylene blue lactose medium, and Hektoen medium, about 500mL each, aliquoted, sterilized at 121°C for 15 minutes; pour plate: beef extract peptone medium, Sabouraud dextrose peptone agar, yeast ...

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Abstract

The invention relates to the technical field of nutritious food production, and in particular to a method for preparing fermented coffee by using intestinal bacteria of cats. The method comprises steps of separating and purifying and identifying intestinal flora of cats by taking cat feces as a raw material, obtaining amplified DNA of purified strains by PCR amplification technology and sequencing, comparing database sequences, further determining the strains to be separated, and identifying the strains as A, B, C and D respectively, inoculating the separated strains on coffee beans in proportion, and performing fermentation and vacuum treatment, so that the prepared coffee has a high content of tryptophan, a low concentration of caffeine, a normal color, and a unique flavor. The fermentedcoffee prepared by the method contains high concentration of tryptophan, the tryptophan is a precursor substance of serotonin, and the serotonin is a nerve conduction substance, can make people relaxand feel happy, slow down nerve activity to cause drowsiness.

Description

technical field [0001] The invention relates to the technical field of nutritious food production, in particular to a method for producing fermented coffee using intestinal bacteria of cats. Background technique [0002] Coffee is consumed by one-third of the world's population, more than any other beverage. There is 1.3% caffeine in roasted coffee and 0.8% caffeine in instant coffee. Caffeine stimulates the central nervous system, has a diuretic effect, and also has the effect of relaxing smooth muscle and dilating peripheral blood vessels. In addition, it also has an analgesic effect because of its prostaglandin synthesis inhibitory effect. Therefore, it is effective for bronchial asthma, migraine, neuralgia, etc. [0003] Coffee has many benefits for the human body: coffee contains certain nutrients, the niacin of coffee contains vitamin B, and the content of roasted coffee beans is higher. And there are free fatty acids, caffeine, tannic acid, etc.; coffee is good fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F5/10C12N1/00C12N1/02
CPCA23F5/10C12N1/00C12N1/02
Inventor 何方舟王亮
Owner 肽晟堂生物科技(常州)有限公司
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