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Preparation method of protein peptide copper chelate suitable for ruminants

A copper chelate and ruminant technology, applied in the field of preparation of protein peptide copper chelate, can solve problems such as low absorption and utilization rate, and achieve the effects of high production efficiency, simple process flow and low cost

Inactive Publication Date: 2020-05-01
陈石良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the common copper supplements on the market are mainly inorganic copper. In the process of digestion and absorption, inorganic copper ions are easily affected by phytic acid, amino acids, cellulose, sugar complexes, vitamins, and unsaturated fatty acids in the feed, resulting in absorption and utilization. low rate

Method used

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  • Preparation method of protein peptide copper chelate suitable for ruminants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Preparation of protein solution: Weigh 100 kg of soybean protein isolate powder (crude protein content is 92%), add 800 kg of water, stir evenly, and obtain protein solution;

[0019] (2) Enzymolysis: adjust the pH value of the protein solution to 8.0, add 1.5 kg of protease, and enzymolyze at 55°C for 8 hours, then raise the temperature of the enzymolysis solution to 85°C to inactivate the enzyme for 10 minutes to obtain the enzymolysis solution;

[0020] (3) Ultrafiltration: The enzymatic hydrolysis solution is subjected to ultrafiltration through an ultrafiltration membrane device with a molecular weight cut-off of 3000Da, and the permeate is collected to obtain a protein peptide solution;

[0021] (4) Chelation reaction: Add 5 kg of copper sulfate to the protein peptide solution, adjust the pH value of the solution to 5.5 under constant stirring, raise the temperature to 80°C, and keep it warm for 2 hours to obtain the protein peptide copper chelation reaction so...

Embodiment 2

[0024] (1) Preparation of protein solution: Weigh 100 kg of soybean protein isolate powder (crude protein content is 90%), add 550 kg of water, stir evenly, and obtain protein solution;

[0025] (2) Enzymolysis: adjust the pH value of the protein solution to 8.5, add 1.8 kg of protease, and enzymolyze at 52°C for 5 hours, then raise the temperature of the enzymolysis solution to 85°C to inactivate the enzyme for 10 minutes to obtain the enzymolysis solution;

[0026] (3) Ultrafiltration: The enzymatic hydrolysis solution is subjected to ultrafiltration through an ultrafiltration membrane device with a molecular weight cut-off of 3000Da, and the permeate is collected to obtain a protein peptide solution;

[0027] (4) Chelation reaction: Add 8 kg of copper sulfate to the protein peptide solution, adjust the pH value of the solution to 5.8 under constant stirring, raise the temperature to 90°C, and keep it warm for 3 hours to obtain the protein peptide copper chelation reaction so...

Embodiment 3

[0030] (1) Preparation of protein solution: Weigh 100 kg of soybean protein isolate powder (crude protein content is 91%), add 600 kg of water, stir evenly, and obtain protein solution;

[0031] (2) Enzymolysis: adjust the pH value of the protein solution to 8.2, add 1.62 kg of protease, and enzymolyze at 50°C for 6 hours, then raise the temperature of the enzymolysis solution to 85°C to inactivate the enzyme for 10 minutes to obtain the enzymolysis solution;

[0032] (3) Ultrafiltration: The enzymatic hydrolysis solution is subjected to ultrafiltration through an ultrafiltration membrane device with a molecular weight cut-off of 3000Da, and the permeate is collected to obtain the ultrafiltration permeate;

[0033] (4) Chelation reaction: add 10 kg of copper sulfate to the protein peptide solution, adjust the pH value of the solution to 6.0 under constant stirring, raise the temperature to 85°C, and keep it warm for 2.5 hours to obtain the protein peptide copper chelation react...

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Abstract

The invention relates to a preparation method of a protein peptide copper chelate suitable for ruminants. The method comprises main steps of protein liquid preparation, enzymolysis, ultrafiltration, chelation and spray drying so as to obtain the protein peptide copper chelate. The method takes soybean protein wide in source as a protein peptide source and inorganic copper salt as an iron source, chelates copper element and protein peptide, and is simple in technological process, low in cost and suitable for large-scale industrial production. The product prepared by the method has high contentof micromolecular peptide and high content of chelated copper ion, and is a novel efficient copper supplement agent for feeds.

Description

technical field [0001] The invention relates to a preparation method of organic trace elements, in particular to a preparation method of protein peptide copper chelate suitable for ruminants. Background technique [0002] Copper is an essential trace element for animals. Its main function is to participate in the process of hematopoiesis, enhance disease resistance, and participate in the formation of pigments. When the copper content decreases in the body, it will affect the absorption of iron, resulting in iron utilization disorders, and eventually iron deficiency anemia. Copper deficiency can also cause mild loosening of bones and other defects. Copper-deficient females have reproductive dysfunction, premature embryo death, low ovarian function in copper-deficient dairy cows, delayed or blocked estrus, low conception rate, difficult childbirth, retained placenta, and congenital rickets in calves. The conception rate of cows with low blood copper levels increased after c...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14C07K1/34
CPCC07K1/14C07K1/34C12P21/06
Inventor 陈石良
Owner 陈石良
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